Determinants encoding fimbriae type 1 in fecal Escherichia coli
are associated with increased frequency of bacteriocinogeny

J 2015

Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny

ŠTAUDOVÁ, Barbora; Lenka MICENKOVÁ; Juraj BOSÁK; Kristýna HRAZDILOVÁ; Eva SLANINKOVÁ et al.

Základní údaje

Originální název

Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny

Autoři

ŠTAUDOVÁ, Barbora; Lenka MICENKOVÁ; Juraj BOSÁK; Kristýna HRAZDILOVÁ; Eva SLANINKOVÁ; Martin VRBA; Alena ŠEVČÍKOVÁ; Darina KOHOUTOVÁ; Vladana WOZNICOVÁ; Jan BUREŠ a David ŠMAJS

Vydání

BMC Microbiology, London, BioMed Central, 2015, 1471-2180

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.581

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/15:00087463

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Escherichia coli; Colicin; Microcin; Bacteriocin; Type 1 fimbriae; Phylogenetic group

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 24. 11. 2015 15:52, Soňa Böhmová

Anotace

V originále

Background: To screen whether E. coli strains encoding type 1 fimbriae, isolated from fecal microflora, produce bacteriocins more often relative to fimA-negative E. coli strains of similar origin. Methods: PCR assays were used to detect presence of genes encoding 30 bacteriocin determinants (23 colicin-and 7 microcin-encoding genes) and 18 virulence determinants in 579 E. coli strains of human and animal origin isolated from hospitals and animal facilities in the Czech and Slovak Republic. E. coli strains were also classified into phylogroups (A, B1, B2 and D). Results: fimA-negative E. coli strains (defined as those possessing none of the 18 tested virulence determinants) were compared to fimA-positive E. coli strains (possessing fimA as the only detected virulence determinant). Strains with identified bacteriocin genes were more commonly found among fimA-positive E. coli strains (35.6 %) compared to fimA-negative E. coli strains (21.9 %, p < 0.01) and this was true for both colicin and microcin determinants (p = 0.02 and p < 0.01, respectively). In addition, an increased number of strains encoding colicin E1 were found among fimA-positive E. coli strains (p < 0.01). Conclusions: fimA-positive E. coli strains produced bacteriocins (colicins and microcins) more often compared to fimA-negative strains of similar origin. Since type 1 fimbriae of E. coli have been shown to mediate adhesion to epithelial host cells and help colonize the intestines, bacteriocin synthesis appears to be an additional feature of colonizing E. coli strains.

Návaznosti

NT13413, projekt VaV

Název: Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Investor: Ministerstvo zdravotnictví ČR, Stanovení apoptózy v biopticky odebraných vzorcích z tlustého střeva

Citovat

ŠTAUDOVÁ, Barbora; Lenka MICENKOVÁ; Juraj BOSÁK; Kristýna HRAZDILOVÁ; Eva SLANINKOVÁ; Martin VRBA; Alena ŠEVČÍKOVÁ; Darina KOHOUTOVÁ; Vladana WOZNICOVÁ; Jan BUREŠ a David ŠMAJS. Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny. BMC Microbiology. London: BioMed Central, 2015, roč. 15, č. 201, s. 1-9. ISSN 1471-2180. Dostupné z: https://doi.org/10.1186/s12866-015-0530-5.

@article{1317917,
   author = {Štaudová, Barbora and Micenková, Lenka and Bosák, Juraj and Hrazdilová, Kristýna and Slaninková, Eva and Vrba, Martin and Ševčíková, Alena and Kohoutová, Darina and Woznicová, Vladana and Bureš, Jan and Šmajs, David},
   article_location = {London},
   article_number = {201},
   doi = {https://doi.org/10.1186/s12866-015-0530-5},
   keywords = {Escherichia coli; Colicin; Microcin; Bacteriocin; Type 1 fimbriae; Phylogenetic group},
   language = {eng},
   issn = {1471-2180},
   journal = {BMC Microbiology},
   title = {Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny},
   volume = {15},
   year = {2015}
}

TY  - JOUR
ID  - 1317917
AU  - Štaudová, Barbora - Micenková, Lenka - Bosák, Juraj - Hrazdilová, Kristýna - Slaninková, Eva - Vrba, Martin - Ševčíková, Alena - Kohoutová, Darina - Woznicová, Vladana - Bureš, Jan - Šmajs, David
PY  - 2015
TI  - Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny
JF  - BMC Microbiology
VL  - 15
IS  - 201
SP  - 1-9
EP  - 1-9
PB  - BioMed Central
SN  - 14712180
KW  - Escherichia coli
KW  - Colicin
KW  - Microcin
KW  - Bacteriocin
KW  - Type 1 fimbriae
KW  - Phylogenetic group
N2  - Background: To screen whether E. coli strains encoding type 1 fimbriae, isolated from fecal microflora, produce bacteriocins more often relative to fimA-negative E. coli strains of similar origin. Methods: PCR assays were used to detect presence of genes encoding 30 bacteriocin determinants (23 colicin-and 7 microcin-encoding genes) and 18 virulence determinants in 579 E. coli strains of human and animal origin isolated from hospitals and animal facilities in the Czech and Slovak Republic. E. coli strains were also classified into phylogroups (A, B1, B2 and D). Results: fimA-negative E. coli strains (defined as those possessing none of the 18 tested virulence determinants) were compared to fimA-positive E. coli strains (possessing fimA as the only detected virulence determinant). Strains with identified bacteriocin genes were more commonly found among fimA-positive E. coli strains (35.6 %) compared to fimA-negative E. coli strains (21.9 %, p < 0.01) and this was true for both colicin and microcin determinants (p = 0.02 and p < 0.01, respectively). In addition, an increased number of strains encoding colicin E1 were found among fimA-positive E. coli strains (p < 0.01). Conclusions: fimA-positive E. coli strains produced bacteriocins (colicins and microcins) more often compared to fimA-negative strains of similar origin. Since type 1 fimbriae of E. coli have been shown to mediate adhesion to epithelial host cells and help colonize the intestines, bacteriocin synthesis appears to be an additional feature of colonizing E. coli strains.
ER  -

ŠTAUDOVÁ, Barbora; Lenka MICENKOVÁ; Juraj BOSÁK; Kristýna HRAZDILOVÁ; Eva SLANINKOVÁ; Martin VRBA; Alena ŠEVČÍKOVÁ; Darina KOHOUTOVÁ; Vladana WOZNICOVÁ; Jan BUREŠ a David ŠMAJS. Determinants encoding fimbriae type 1 in fecal Escherichia coli are associated with increased frequency of bacteriocinogeny. \textit{BMC Microbiology}. London: BioMed Central, 2015, roč.~15, č.~201, s.~1-9. ISSN~1471-2180. Dostupné z: https://doi.org/10.1186/s12866-015-0530-5.

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