Identification of Borrelia burgdorferi genospecies isolated
from Ixodes ricinus ticks in the South Moravian region of the
Czech Republic

J 2015

Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic

BONCZEK, Ondřej; Alena ŽÁKOVSKÁ; Lýdia HLINECKÁ a Omar ŠERÝ

Základní údaje

Originální název

Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic

Autoři

BONCZEK, Ondřej; Alena ŽÁKOVSKÁ; Lýdia HLINECKÁ a Omar ŠERÝ

Vydání

Annals of Agricultural and Environmental Medicine, Institute of Agricultural Medicine, 2015, 1232-1966

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 0.895

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00085836

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Borrelia burgdorferi s. l.; Borrelia spielmanii; Ixodes ricinus; genotyping; RealTime PCR; DNA sequencing

Štítky

AKR, rivok

Změněno: 2. 3. 2016 19:29, prof. RNDr. Omar Šerý, Ph.D.

Anotace

V originále

During 2008-2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ). The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database. A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also observed: B. afzelii (70.0%), B. garinii (10.0%), B. valaisiana (8.6%), and B. burgdorferi s. s. (4.3%). A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of B. spielmanii for the first time in this region.

Citovat

BONCZEK, Ondřej; Alena ŽÁKOVSKÁ; Lýdia HLINECKÁ a Omar ŠERÝ. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. Annals of Agricultural and Environmental Medicine. Institute of Agricultural Medicine, 2015, roč. 22, č. 4, s. 637-641. ISSN 1232-1966. Dostupné z: https://doi.org/10.5604/12321966.1185766.

@article{1323845,
   author = {Bonczek, Ondřej and Žákovská, Alena and Hlinecká, Lýdia and Šerý, Omar},
   article_number = {4},
   doi = {https://doi.org/10.5604/12321966.1185766},
   keywords = {Borrelia burgdorferi s. l.; Borrelia spielmanii; Ixodes ricinus; genotyping; RealTime PCR; DNA sequencing},
   language = {eng},
   issn = {1232-1966},
   journal = {Annals of Agricultural and Environmental Medicine},
   title = {Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic},
   url = {http://aaem.pl/fulltxt.php?ICID=1185766},
   volume = {22},
   year = {2015}
}

TY  - JOUR
ID  - 1323845
AU  - Bonczek, Ondřej - Žákovská, Alena - Hlinecká, Lýdia - Šerý, Omar
PY  - 2015
TI  - Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic
JF  - Annals of Agricultural and Environmental Medicine
VL  - 22
IS  - 4
SP  - 637-641
EP  - 637-641
PB  - Institute of Agricultural Medicine
SN  - 12321966
KW  - Borrelia burgdorferi s. l.
KW  - Borrelia spielmanii
KW  - Ixodes ricinus
KW  - genotyping
KW  - RealTime PCR
KW  - DNA sequencing
UR  - http://aaem.pl/fulltxt.php?ICID=1185766
N2  - During 2008-2012, a total of 466 ticks Ixodes ricinus removed from humans were collected and tested for the presence of Borrelia burgdorferi sensu lato (Bbsl). Ticks were collected in all districts of the South Moravian region of the Czech Republic (CZ). The aim of this study was to determine the infestation of Bbsl in ticks Ixodes ricinus and the identification of genospecies of Bbsl group by DNA sequencing. DNA isolation from homogenates was performed by UltraClean BloodSpin DNA kit (MoBio) and by automated instrument Prepito (Perkin-Elmer). Detection of spirochetes was carried out by RealTime PCR kit EliGene Borrelia LC (Elisabeth Pharmacon). Finally, all the positive samples were sequenced on an ABI 3130 Genetic Analyzer (Life Technologies) and identified in the BLAST (NCBI) database. A total positivity of the samples was 26%. For the first time in the Czech Republic, 5 of the isolated strains were genotyped as Borrelia spielmanii (7.1%). Other representatives of Bbsl were also observed: B. afzelii (70.0%), B. garinii (10.0%), B. valaisiana (8.6%), and B. burgdorferi s. s. (4.3%). A general view of the spreading of Bbsl in the South Moravian region was demonstrated. The most interesting result of the study is the finding of B. spielmanii for the first time in this region.
ER  -

BONCZEK, Ondřej; Alena ŽÁKOVSKÁ; Lýdia HLINECKÁ a Omar ŠERÝ. Identification of Borrelia burgdorferi genospecies isolated from Ixodes ricinus ticks in the South Moravian region of the Czech Republic. \textit{Annals of Agricultural and Environmental Medicine}. Institute of Agricultural Medicine, 2015, roč.~22, č.~4, s.~637-641. ISSN~1232-1966. Dostupné z: https://doi.org/10.5604/12321966.1185766.

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