Immunomodulatory Potency of Microcystin, an Important
Water-Polluting Cyanobacterial Toxin

J 2015

Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin

ADAMOVSKÝ, Ondřej; Zdena MOOSOVÁ; Michaela PEKAROVA; Amrita BASU; Pavel BABICA et al.

Základní údaje

Originální název

Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin

Autoři

ADAMOVSKÝ, Ondřej; Zdena MOOSOVÁ; Michaela PEKAROVA; Amrita BASU; Pavel BABICA; Lenka ŠINDLEROVÁ; Lukáš KUBALA a Luděk BLÁHA

Vydání

Environmental Science and Technology, WASHINGTON, DC (USA), American Chemical Society, 2015, 0013-936X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.393

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/15:00081602

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

NF-KAPPA-B; NECROSIS-FACTOR-ALPHA; TOLL-LIKE RECEPTORS; ANION TRANSPORTING POLYPEPTIDES; ACTIVATED PROTEIN-KINASE; MELANOMA-CELL INVASION; LIVER-TUMOR PROMOTION; NITRIC-OXIDE; SIGNALING PATHWAYS; IN-VITRO

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 6. 2025 08:52, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin-6) as a consequence of fast nuclear factor kappa B and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses. Taken together, we showed for the first time that MC-LR could interfere with macrophage receptors that are responsible for triggering the above-mentioned signaling pathways. These findings provide an interesting mechanistic explanation of some adverse health outcomes associated with toxic cyanobacteria and MCs.

Návaznosti

GP13-27695P, projekt VaV

Název: Metabolity sinic jako možné modulátory imunitního systému (Akronym: immunotox)

Investor: Grantová agentura ČR, Metabolity sinic jako možné modulátory immunitního systému

Citovat

ADAMOVSKÝ, Ondřej; Zdena MOOSOVÁ; Michaela PEKAROVA; Amrita BASU; Pavel BABICA; Lenka ŠINDLEROVÁ; Lukáš KUBALA a Luděk BLÁHA. Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin. Environmental Science and Technology. WASHINGTON, DC (USA): American Chemical Society, 2015, roč. 49, č. 20, s. 12457-12464. ISSN 0013-936X. Dostupné z: https://doi.org/10.1021/acs.est.5b02049.

@article{1336287,
   author = {Adamovský, Ondřej and Moosová, Zdena and Pekarova, Michaela and Basu, Amrita and Babica, Pavel and Šindlerová, Lenka and Kubala, Lukáš and Bláha, Luděk},
   article_location = {WASHINGTON, DC (USA)},
   article_number = {20},
   doi = {https://doi.org/10.1021/acs.est.5b02049},
   keywords = {NF-KAPPA-B; NECROSIS-FACTOR-ALPHA; TOLL-LIKE RECEPTORS; ANION TRANSPORTING POLYPEPTIDES; ACTIVATED PROTEIN-KINASE; MELANOMA-CELL INVASION; LIVER-TUMOR PROMOTION; NITRIC-OXIDE; SIGNALING PATHWAYS; IN-VITRO},
   language = {eng},
   issn = {0013-936X},
   journal = {Environmental Science and Technology},
   title = {Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin},
   url = {http://pubs.acs.org/doi/10.1021/acs.est.5b02049},
   volume = {49},
   year = {2015}
}

TY  - JOUR
ID  - 1336287
AU  - Adamovský, Ondřej - Moosová, Zdena - Pekarova, Michaela - Basu, Amrita - Babica, Pavel - Šindlerová, Lenka - Kubala, Lukáš - Bláha, Luděk
PY  - 2015
TI  - Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin
JF  - Environmental Science and Technology
VL  - 49
IS  - 20
SP  - 12457-12464
EP  - 12457-12464
PB  - American Chemical Society
SN  - 0013936X
KW  - NF-KAPPA-B
KW  - NECROSIS-FACTOR-ALPHA
KW  - TOLL-LIKE RECEPTORS
KW  - ANION TRANSPORTING POLYPEPTIDES
KW  - ACTIVATED PROTEIN-KINASE
KW  - MELANOMA-CELL INVASION
KW  - LIVER-TUMOR PROMOTION
KW  - NITRIC-OXIDE
KW  - SIGNALING PATHWAYS
KW  - IN-VITRO
UR  - http://pubs.acs.org/doi/10.1021/acs.est.5b02049
L2  - http://pubs.acs.org/doi/10.1021/acs.est.5b02049
N2  - Microcystins (MCs) are primarily hepatotoxins produced by cyanobacteria and are responsible for intoxication in humans and animals. There are many incidents of chronic exposure to MCs, which have been attributed to the inappropriate treatment of water supplies or contaminated food. Using RAW 264.7 macrophages, we showed the potency of microcystin-LR (MC-LR) to stimulate production of pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin-6) as a consequence of fast nuclear factor kappa B and nitrogen-activated protein kinase activation. In contrast to other studies, the observed effects were not attributed to the intracellular inhibition of protein phosphatases 1/2A due to lack of specific transmembrane transporters for MCs. However, the MC-LR-induced activation of macrophages was effectively inhibited by a specific peptide that blocks signaling of receptors, which play a pivotal role in the innate immune responses. Taken together, we showed for the first time that MC-LR could interfere with macrophage receptors that are responsible for triggering the above-mentioned signaling pathways. These findings provide an interesting mechanistic explanation of some adverse health outcomes associated with toxic cyanobacteria and MCs.
ER  -

ADAMOVSKÝ, Ondřej; Zdena MOOSOVÁ; Michaela PEKAROVA; Amrita BASU; Pavel BABICA; Lenka ŠINDLEROVÁ; Lukáš KUBALA a Luděk BLÁHA. Immunomodulatory Potency of Microcystin, an Important Water-Polluting Cyanobacterial Toxin. \textit{Environmental Science and Technology}. WASHINGTON, DC (USA): American Chemical Society, 2015, roč.~49, č.~20, s.~12457-12464. ISSN~0013-936X. Dostupné z: https://doi.org/10.1021/acs.est.5b02049.

Přehled o publikaci