Dietary fatty acids specifically modulate phospholipid
patternin colon cells with distinct differentiation capacities

J 2017

Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities

HOFMANOVÁ, Jiřina; Josef SLAVÍK; Petra OVESNÁ; Zuzana TYLICHOVÁ; Jan VONDRÁČEK et al.

Základní údaje

Originální název

Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities

Autoři

Vydání

European Journal of Nutrition, Springer, 2017, 1436-6207

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.423

Kód RIV

RIV/00216224:14310/17:00095836

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1007/s00394-016-1196-y

UT WoS

000403469100011

EID Scopus

2-s2.0-84961203424

Klíčová slova česky

Apoptóza; butyrát; kardiolipiny; rakovina střeva; dokosahexanová kyselina; fosfolipidy

Klíčová slova anglicky

Apoptosis; Butyrate; Cardiolipins; Colon cancer; Docosahexaenoic acid; Phospholipids

Štítky

AKR, NZ, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 21. 2. 2018 10:08, Ing. Nicole Zrilić

Anotace

V originále

Although beneficial effects of the dietary n-3 docosahexaenoic acid (DHA) or butyrate in colon carcinogenesis have been implicated, the mechanisms of their action are not fully clear. Here, we investigated modulations of composition of individual phospholipid (PL) classes, with a particular emphasis on cardiolipins (CLs), in colon cells treated with DHA, sodium butyrate (NaBt), or their combination (DHA/NaBt), and we evaluated possible associations between lipid changes and cell fate after fatty acid treatment. In two distinct human colon cell models, foetal colon (FHC) and adenocarcinoma (HCT-116) cells, we compared patterns and composition of individual PL classes following the fatty acid treatment by HPLC-MS/MS. In parallel, we measured the parameters reflecting cell proliferation, differentiation and death. In FHC cells, NaBt induced primarily differentiation, while co-treatment with DHA shifted their response towards cell death. In contrast, NaBt induced apoptosis in HCT-116 cells, which was not further affected by DHA. DHA was incorporated in all main PL types, increasing their unsaturation, while NaBt did not additionally modulate these effects in either cell model. Nevertheless, we identified an unusually wide range of CL species to be highly increased by NaBt and particularly by DHA/NaBt, and these effects were more pronounced in HCT-116 cells. DHA and DHA/NaBt enhanced levels of high molecular weight and more unsaturated CL species, containing DHA, which was specific for either differentiation or apoptotic responses. We identified a wide range of CL species in the colon cells which composition was significantly modified after DHA and NaBt treatment. These specific CL modulations might contribute to distinct cellular differentiation or apoptotic responses.

Citovat

HOFMANOVÁ, Jiřina; Josef SLAVÍK; Petra OVESNÁ; Zuzana TYLICHOVÁ; Jan VONDRÁČEK; Nicol STRAKOVÁ; Alena HYRŠLOVÁ VACULOVÁ; Miroslav CIGANEK; Alois KOZUBÍK; Lucia KNOPFOVÁ; Jan ŠMARDA a Miroslav MACHALA. Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities. European Journal of Nutrition. Springer, 2017, roč. 56, č. 4, s. 1493-1508. ISSN 1436-6207. Dostupné z: https://doi.org/10.1007/s00394-016-1196-y.

@article{1340797,
   author = {Hofmanová, Jiřina and Slavík, Josef and Ovesná, Petra and Tylichová, Zuzana and Vondráček, Jan and Straková, Nicol and Hyršlová Vaculová, Alena and Ciganek, Miroslav and Kozubík, Alois and Knopfová, Lucia and Šmarda, Jan and Machala, Miroslav},
   article_number = {4},
   doi = {https://doi.org/10.1007/s00394-016-1196-y},
   keywords = {Apoptosis; Butyrate; Cardiolipins; Colon cancer; Docosahexaenoic acid; Phospholipids},
   language = {eng},
   issn = {1436-6207},
   journal = {European Journal of Nutrition},
   title = {Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities},
   volume = {56},
   year = {2017}
}

TY  - JOUR
ID  - 1340797
AU  - Hofmanová, Jiřina - Slavík, Josef - Ovesná, Petra - Tylichová, Zuzana - Vondráček, Jan - Straková, Nicol - Hyršlová Vaculová, Alena - Ciganek, Miroslav - Kozubík, Alois - Knopfová, Lucia - Šmarda, Jan - Machala, Miroslav
PY  - 2017
TI  - Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities
JF  - European Journal of Nutrition
VL  - 56
IS  - 4
SP  - 1493-1508
EP  - 1493-1508
PB  - Springer
SN  - 14366207
KW  - Apoptosis
KW  - Butyrate
KW  - Cardiolipins
KW  - Colon cancer
KW  - Docosahexaenoic acid
KW  - Phospholipids
N2  - Although beneficial effects of the dietary n-3 docosahexaenoic acid (DHA) or butyrate in colon carcinogenesis have been implicated, the mechanisms of their action are not fully clear. Here, we investigated modulations of composition of individual phospholipid (PL) classes, with a particular emphasis on cardiolipins (CLs), in colon cells treated with DHA, sodium butyrate (NaBt), or their combination (DHA/NaBt), and we evaluated possible associations between lipid changes and cell fate after fatty acid treatment. In two distinct human colon cell models, foetal colon (FHC) and adenocarcinoma (HCT-116) cells, we compared patterns and composition of individual PL classes following the fatty acid treatment by HPLC-MS/MS. In parallel, we measured the parameters reflecting cell proliferation, differentiation and death. In FHC cells, NaBt induced primarily differentiation, while co-treatment with DHA shifted their response towards cell death. In contrast, NaBt induced apoptosis in HCT-116 cells, which was not further affected by DHA. DHA was incorporated in all main PL types, increasing their unsaturation, while NaBt did not additionally modulate these effects in either cell model. Nevertheless, we identified an unusually wide range of CL species to be highly increased by NaBt and particularly by DHA/NaBt, and these effects were more pronounced in HCT-116 cells. DHA and DHA/NaBt enhanced levels of high molecular weight and more unsaturated CL species, containing DHA, which was specific for either differentiation or apoptotic responses. We identified a wide range of CL species in the colon cells which composition was significantly modified after DHA and NaBt treatment. These specific CL modulations might contribute to distinct cellular differentiation or apoptotic responses.
ER  -

HOFMANOVÁ, Jiřina; Josef SLAVÍK; Petra OVESNÁ; Zuzana TYLICHOVÁ; Jan VONDRÁČEK; Nicol STRAKOVÁ; Alena HYRŠLOVÁ VACULOVÁ; Miroslav CIGANEK; Alois KOZUBÍK; Lucia KNOPFOVÁ; Jan ŠMARDA a Miroslav MACHALA. Dietary fatty acids specifically modulate phospholipid patternin colon cells with distinct differentiation capacities. \textit{European Journal of Nutrition}. Springer, 2017, roč.~56, č.~4, s.~1493-1508. ISSN~1436-6207. Dostupné z: https://doi.org/10.1007/s00394-016-1196-y.

Přehled o publikaci