Effect of glucose variability on pathways associated with
glucotoxicity in diabetes: Evaluation of a novel in vitro
experimental approach

J 2016

Effect of glucose variability on pathways associated with glucotoxicity in diabetes: Evaluation of a novel in vitro experimental approach

KURICOVÁ, Katarína; Lukáš PÁCAL; Jan ŠOUPAL; Martin PRÁZNÝ; Kateřina KAŇKOVÁ et al.

Základní údaje

Originální název

Effect of glucose variability on pathways associated with glucotoxicity in diabetes: Evaluation of a novel in vitro experimental approach

Autoři

KURICOVÁ, Katarína; Lukáš PÁCAL; Jan ŠOUPAL; Martin PRÁZNÝ a Kateřina KAŇKOVÁ

Vydání

Diabetes Research and Clinical Practice, Clare, Elsevier Ireland Ltd. 2016, 0168-8227

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.639

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00088852

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Glycaemic variability; Continuous glucose monitoring; Diabetes complications; Hyperglycaemia; Hypoglycaemia; Pentose phosphate pathway

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 4. 2017 10:33, Soňa Böhmová

Anotace

V originále

Aims: Glycaemic variability (GV) has been hypothesized to increase the risk of diabetes complications; however, results of clinical studies are contradictory. The effect of GV on cell phenotypes has been investigated in vitro showing that GV may have more deleterious effect on cells that high glucose itself. However, methodology used to study GV in vitro differs significantly between studies and does not reflect in vivo situation. Therefore we aimed to establish clinically relevant an in vitro experimental approach for the study of GV that reflects intra-day glucose fluctuations of subjects with type 1 diabetes mellitus (T1DM) and of healthy subjects and to test how low and high GV affect expression of genes that protects cells from hyperglycaemia-induced damage. Methods: Human umbilical vein endothelial cells (HUVEC) were cultured 24 h in medium with different glucose profiles: high GV, low GV and GV of healthy subjects—profiles created according to CGM of T1DM patients and healthy subjects. These profiles were compared to commonly used 5.5 and 25 mmol/l glucose concentrations. Gene expression was determined using quantitative PCR. Results: Our results showed general down-regulation of enzymes that are involved in the protection against hyperglycaemia-induced intracellular changes in both low and high GV compared to normal glycaemia similarly to the decrease induced by continuous hyperglycaemia. Gene expressions did not differ between high and low GV. Conclusion: Our data indicate that GV may have similar or even greater effect than continuous hyperglycaemia on the expression of several genes relevant to pathogenesis of diabetes microvascular complications.

Návaznosti

NT13198, projekt VaV

Název: Pentózový cyklus jako potenciální nový terapeutický cíl v prevenci diabetických komplikací

Investor: Ministerstvo zdravotnictví ČR, Pentózový cyklus jako potenciální nový terapeutický cíl v prevenci diabetických komplikací

Citovat

KURICOVÁ, Katarína; Lukáš PÁCAL; Jan ŠOUPAL; Martin PRÁZNÝ a Kateřina KAŇKOVÁ. Effect of glucose variability on pathways associated with glucotoxicity in diabetes: Evaluation of a novel in vitro experimental approach. Diabetes Research and Clinical Practice. Clare: Elsevier Ireland Ltd., 2016, roč. 114, APR 01, s. 1-8. ISSN 0168-8227. Dostupné z: https://doi.org/10.1016/j.diabres.2016.02.006.

@article{1343740,
   author = {Kuricová, Katarína and Pácal, Lukáš and Šoupal, Jan and Prázný, Martin and Kaňková, Kateřina},
   article_location = {Clare},
   article_number = {APR 01},
   doi = {https://doi.org/10.1016/j.diabres.2016.02.006},
   keywords = {Glycaemic variability; Continuous glucose monitoring; Diabetes complications; Hyperglycaemia; Hypoglycaemia; Pentose phosphate pathway},
   language = {eng},
   issn = {0168-8227},
   journal = {Diabetes Research and Clinical Practice},
   title = {Effect of glucose variability on pathways associated with glucotoxicity in diabetes: Evaluation of a novel in vitro experimental approach},
   volume = {114},
   year = {2016}
}

TY  - JOUR
ID  - 1343740
AU  - Kuricová, Katarína - Pácal, Lukáš - Šoupal, Jan - Prázný, Martin - Kaňková, Kateřina
PY  - 2016
TI  - Effect of glucose variability on pathways associated with glucotoxicity in diabetes: Evaluation of a novel in vitro experimental approach
JF  - Diabetes Research and Clinical Practice
VL  - 114
IS  - APR 01
SP  - 1-8
EP  - 1-8
PB  - Elsevier Ireland Ltd.
SN  - 01688227
KW  - Glycaemic variability
KW  - Continuous glucose monitoring
KW  - Diabetes complications
KW  - Hyperglycaemia
KW  - Hypoglycaemia
KW  - Pentose phosphate pathway
N2  - Aims: Glycaemic variability (GV) has been hypothesized to increase the risk of diabetes complications; however, results of clinical studies are contradictory. The effect of GV on cell phenotypes has been investigated in vitro showing that GV may have more deleterious effect on cells that high glucose itself. However, methodology used to study GV in vitro differs significantly between studies and does not reflect in vivo situation. Therefore we aimed to establish clinically relevant an in vitro experimental approach for the study of GV that reflects intra-day glucose fluctuations of subjects with type 1 diabetes mellitus (T1DM) and of healthy subjects and to test how low and high GV affect expression of genes that protects cells from hyperglycaemia-induced damage. Methods: Human umbilical vein endothelial cells (HUVEC) were cultured 24 h in medium with different glucose profiles: high GV, low GV and GV of healthy subjects—profiles created according to CGM of T1DM patients and healthy subjects. These profiles were compared to commonly used 5.5 and 25 mmol/l glucose concentrations. Gene expression was determined using quantitative PCR. Results: Our results showed general down-regulation of enzymes that are involved in the protection against hyperglycaemia-induced intracellular changes in both low and high GV compared to normal glycaemia similarly to the decrease induced by continuous hyperglycaemia. Gene expressions did not differ between high and low GV. Conclusion: Our data indicate that GV may have similar or even greater effect than continuous hyperglycaemia on the expression of several genes relevant to pathogenesis of diabetes microvascular complications.
ER  -

KURICOVÁ, Katarína; Lukáš PÁCAL; Jan ŠOUPAL; Martin PRÁZNÝ a Kateřina KAŇKOVÁ. Effect of glucose variability on pathways associated with glucotoxicity in diabetes: Evaluation of a novel in vitro experimental approach. \textit{Diabetes Research and Clinical Practice}. Clare: Elsevier Ireland Ltd., 2016, roč.~114, APR 01, s.~1-8. ISSN~0168-8227. Dostupné z: https://doi.org/10.1016/j.diabres.2016.02.006.

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