MALDI TOF MS imaging of 3D neuroblastoma cell cultures

k 2016

MALDI TOF MS imaging of 3D neuroblastoma cell cultures

MACHÁLKOVÁ, Markéta; Adam PRUŠKA; Jarmila NAVRÁTILOVÁ; Jan ŠMARDA; Jan PREISLER et al.

Základní údaje

Originální název

MALDI TOF MS imaging of 3D neuroblastoma cell cultures

Název česky

MALDI TOF MS zobrazování 3D neuroblastomových buněčných kultur

Autoři

MACHÁLKOVÁ, Markéta; Adam PRUŠKA; Jarmila NAVRÁTILOVÁ; Jan ŠMARDA a Jan PREISLER

Vydání

5. Konference České společnosti pro hmotnostní spektrometrii, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00087892

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-905045-6-1

Klíčová slova anglicky

MS; imaging; spheroid; 3D neuroblastoma cell culture; perifosine; metaiodobenzylguanidine; matrix deposition

Změněno: 19. 12. 2016 17:17, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206. Initial images showing distribution of the drugs and selected lipids with CHCA and DHB matrices were acquired.

Návaznosti

GA15-05387S, projekt VaV

Název: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

Investor: Grantová agentura ČR, Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MACHÁLKOVÁ, Markéta; Adam PRUŠKA; Jarmila NAVRÁTILOVÁ; Jan ŠMARDA a Jan PREISLER. MALDI TOF MS imaging of 3D neuroblastoma cell cultures. In 5. Konference České společnosti pro hmotnostní spektrometrii. 2016. ISBN 978-80-905045-6-1.

@proceedings{1343761,
   author = {Machálková, Markéta and Pruška, Adam and Navrátilová, Jarmila and Šmarda, Jan and Preisler, Jan},
   booktitle = {5. Konference České společnosti pro hmotnostní spektrometrii},
   keywords = {MS; imaging; spheroid; 3D neuroblastoma cell culture; perifosine; metaiodobenzylguanidine; matrix deposition},
   language = {eng},
   isbn = {978-80-905045-6-1},
   title = {MALDI TOF MS imaging of 3D neuroblastoma cell cultures},
   year = {2016}
}

TY  - CONF
ID  - 1343761
AU  - Machálková, Markéta - Pruška, Adam - Navrátilová, Jarmila - Šmarda, Jan - Preisler, Jan
PY  - 2016
TI  - MALDI TOF MS imaging of 3D neuroblastoma cell cultures
SN  - 9788090504561
KW  - MS
KW  - imaging
KW  - spheroid
KW  - 3D neuroblastoma cell culture
KW  - perifosine
KW  - metaiodobenzylguanidine
KW  - matrix deposition
N2  - Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) has become a routine technique for analyte visualization across biological samples. In a single experiment, it enables imaging both exogenous and endogenous compounds, such as drugs, proteins and lipids. Additional great benefit of this approach is no need for molecule labelling, in comparison with the methods such as fluorescent microscopy, immunohistochemistry etc. In our study, 3D cell cultures SK-N-Be(2) and SH SY5Y were analyzed. The cell formations (spheroids) were embedded in gelatine, frozen, cryo-sectioned into thin slices and thawed to conductive glass slides. For uniform matrix coating, commercial iMatrixSpray sprayer was employed, its parameters were optimized and the results were compared with sublimation method. Developed protocols were applied to the analysis of spheroids treated by potential cancerostatics metaiodobenzylguanidine, perifosine and MK 2206. Initial images showing distribution of the drugs and selected lipids with CHCA and DHB matrices were acquired.
ER  -

MACHÁLKOVÁ, Markéta; Adam PRUŠKA; Jarmila NAVRÁTILOVÁ; Jan ŠMARDA a Jan PREISLER. MALDI TOF MS imaging of 3D neuroblastoma cell cultures. In \textit{5. Konference České společnosti pro hmotnostní spektrometrii}. 2016. ISBN~978-80-905045-6-1.

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