Exploring the transcriptome of Eudiplozoon nipponicum
(Monogenea, Diplozoidae) adult worm

k 2016

Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm

VOREL, Jiří; Pavel ROUDNICKÝ; Jana ILGOVÁ; Hana DVOŘÁKOVÁ; Lucie JEDLIČKOVÁ et al.

Základní údaje

Originální název

Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm

Autoři

Vydání

XII. české a slovenské parazitologické dny, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00087937

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova česky

NGS, Monogenea, Eudiplozoon nipponicum; transkriptom, bioinformatika, anotace

Klíčová slova anglicky

NGS; Monogenea; Eudiplozoon nipponicum; transcriptome; bioinformatics; annotation

Změněno: 25. 3. 2017 07:33, RNDr. Martin Kašný, Ph.D.

Anotace

V originále

Parasitic monogeneans are blood sucking fish ectoparasites. Their presence can lead to significant losses in fish stocks. From a large group of Monogenea (ca 25 000 species) only two species have been deeply sequenced – genomes of Gyrodactylus salaris and Protopolystoma xenopodis and several monogenean protein molecules have been characterized (e.g. cathepsin L – E. nipponicum and Neobenedenia melleni and three unknown serine peptidases from Neobenedenia girellae). In our study of E. nipponicum transcriptome we adopted two different sequencing approaches. 454 pyrosequencing method (GS FLX System, Roche) and sequencing by synthesis method (GS MiSeq, Illumina) to obtain high quality transcriptomic data. We generated 324 941 reads which were assembled into 6910 contigs (454 GS FLX System) and 149 697 864 reads assembled into almost 10 000 unique sequences (Illumina MiSeq) after processing by specific software tools. In this sequences we identified numerous important protein coding transcripts which can be involved in host-parasite interaction and can be essential for life of monogeneans parasites. We determined e. g. cysteine (cathepsins L1, L3, B, C, S and calpain), serine (prolyl endopeptidase, Kunitz-type peptidases and serine protease 33), metallo (nardilysin, paraplegin, XAA-Pro aminopeptidase) and aspartic (cathepsins D and E-A) proteases, their inhibitors (cystatins, salarin, serpins, stefins, smap1 and Kazal-type inhibitors), tegumental antigens and proteins (STARP antigen), potential anticoagulants agents and immunomodulators. The identified E. nipponicum transcripts are similar with those recorded on other worms from phylum Platyhelminthes.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

VOREL, Jiří; Pavel ROUDNICKÝ; Jana ILGOVÁ; Hana DVOŘÁKOVÁ; Lucie JEDLIČKOVÁ; Libor MIKEŠ; Petr BROŽ; Dagmar JIRSOVÁ; Hynek STRNAD; Roman LEONTOVYČ; Ewa DZIKA; Božena KOUBKOVÁ; Milan GELNAR a Martin KAŠNÝ. Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm. In XII. české a slovenské parazitologické dny. 2016.

@proceedings{1345456,
   author = {Vorel, Jiří and Roudnický, Pavel and Ilgová, Jana and Dvořáková, Hana and Jedličková, Lucie and Mikeš, Libor and Brož, Petr and Jirsová, Dagmar and Strnad, Hynek and Leontovyč, Roman and Dzika, Ewa and Koubková, Božena and Gelnar, Milan and Kašný, Martin},
   booktitle = {XII. české a slovenské parazitologické dny},
   keywords = {NGS; Monogenea; Eudiplozoon nipponicum; transcriptome; bioinformatics; annotation},
   language = {eng},
   title = {Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm},
   year = {2016}
}

TY  - CONF
ID  - 1345456
AU  - Vorel, Jiří - Roudnický, Pavel - Ilgová, Jana - Dvořáková, Hana - Jedličková, Lucie - Mikeš, Libor - Brož, Petr - Jirsová, Dagmar - Strnad, Hynek - Leontovyč, Roman - Dzika, Ewa - Koubková, Božena - Gelnar, Milan - Kašný, Martin
PY  - 2016
TI  - Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm
KW  - NGS
KW  - Monogenea
KW  - Eudiplozoon nipponicum
KW  - transcriptome
KW  - bioinformatics
KW  - annotation
N2  - Parasitic monogeneans are blood sucking fish ectoparasites. Their presence can lead to significant losses in fish stocks. From a large group of Monogenea (ca 25 000 species) only two species have been deeply sequenced – genomes of Gyrodactylus salaris and Protopolystoma xenopodis and several monogenean protein molecules have been characterized (e.g. cathepsin L – E. nipponicum and Neobenedenia melleni and three unknown serine peptidases from Neobenedenia girellae). In our study of E. nipponicum transcriptome we adopted two different sequencing approaches. 454 pyrosequencing method (GS FLX System, Roche) and sequencing by synthesis method (GS MiSeq, Illumina) to obtain high quality transcriptomic data. We generated 324 941 reads which were assembled into 6910 contigs (454 GS FLX System) and 149 697 864 reads assembled into almost 10 000 unique sequences (Illumina MiSeq) after processing by specific software tools. In this sequences we identified numerous important protein coding transcripts which can be involved in host-parasite interaction and can be essential for life of monogeneans parasites. We determined e. g. cysteine (cathepsins L1, L3, B, C, S and calpain), serine (prolyl endopeptidase, Kunitz-type peptidases and serine protease 33), metallo (nardilysin, paraplegin, XAA-Pro aminopeptidase) and aspartic (cathepsins D and E-A) proteases, their inhibitors (cystatins, salarin, serpins, stefins, smap1 and Kazal-type inhibitors), tegumental antigens and proteins (STARP antigen), potential anticoagulants agents and immunomodulators. The identified E. nipponicum transcripts are similar with those recorded on other worms from phylum Platyhelminthes.
ER  -

VOREL, Jiří; Pavel ROUDNICKÝ; Jana ILGOVÁ; Hana DVOŘÁKOVÁ; Lucie JEDLIČKOVÁ; Libor MIKEŠ; Petr BROŽ; Dagmar JIRSOVÁ; Hynek STRNAD; Roman LEONTOVYČ; Ewa DZIKA; Božena KOUBKOVÁ; Milan GELNAR a Martin KAŠNÝ. Exploring the transcriptome of Eudiplozoon nipponicum (Monogenea, Diplozoidae) adult worm. In \textit{XII. české a slovenské parazitologické dny}. 2016.

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