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@misc{1350531,
author = {Škorpíková, Lucie and Ilgová, Jana and Potěšil, David and Zdráhal, Zbyněk and Demko, Martin and Oppelt, Jan and Gelnar, Milan and Kašný, Martin},
booktitle = {EMOP XII – the 12th European Multicolloquium of Parasitology},
keywords = {Eudiplozoon; serpin},
language = {eng},
title = {Deep insight into secretome and transcriptome of Trichinella spiralis and Trichinela pseudospiralis},
url = {http://congress.utu.fi/emop2016/programme.php},
year = {2016}
}
TY - SLIDE
ID - 1350531
AU - Škorpíková, Lucie - Ilgová, Jana - Potěšil, David - Zdráhal, Zbyněk - Demko, Martin - Oppelt, Jan - Gelnar, Milan - Kašný, Martin
PY - 2016
TI - Deep insight into secretome and transcriptome of Trichinella spiralis and Trichinela pseudospiralis
KW - Eudiplozoon
KW - serpin
UR - http://congress.utu.fi/emop2016/programme.php
N2 - By adoption of homology searches of RNA 158 753 271 bases/223 887 transcripts the 9757 contigs (>1 kb) were assembled and particular protein molecules in E. nipponicum transcriptome data were identified, e.g. peptidases/inhibitors; 29 contigs of cysteine peptidases (e.g. cathepsin L) and 7 contigs of their inhibitors (e.g. cystatins); 12 contigs of serine peptidases (e.g. cathepsin A) and 7 contigs of their inhibitors (e.g. serpin). Employing biochemical, proteomic and molecular tools, we found that cysteine peptidase activities prevailed in soluble protein extracts and excretory/secretory (E/S) products of E. nipponicum and the major part of activity was related to cathepsin L-like. Mass spectrometry revealed several tryptic peptides in E/S products matching to two translated sequences of cathepsin L genes. The dominance of cysteine peptidases of cathepsin L type in E. nipponicum resembles the situation in, e.g., fasciolid trematodes. The cathepsin L3 was cloned and expressed in both bacterial and yeasts expression systems. The recombinant enzyme was purified on Ni-NTA agarose column and the experiments focused on its molecular/biochemical properties were started.
ER -
ŠKORPÍKOVÁ, Lucie, Jana ILGOVÁ, David POTĚŠIL, Zbyněk ZDRÁHAL, Martin DEMKO, Jan OPPELT, Milan GELNAR a Martin KAŠNÝ. Deep insight into secretome and transcriptome of Trichinella spiralis and Trichinela pseudospiralis. In \textit{EMOP XII – the 12th European Multicolloquium of Parasitology}. 2016.
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