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@proceedings{1356001,
author = {Ilgová, Jana and Jedličková, Lucie and Dvořáková, Hana and Mikeš, Libor and Janda, Lubomír and Gelnar, Milan and Kašný, Martin},
booktitle = {Europian Multicolloquium On Parasitology},
keywords = {Helminths; Cystatin; immunomodulation; Bloodfeeding},
language = {eng},
title = {Cystatins of helminths: characterization of molecules with immunomodulatory potential},
year = {2016}
}
TY - CONF
ID - 1356001
AU - Ilgová, Jana - Jedličková, Lucie - Dvořáková, Hana - Mikeš, Libor - Janda, Lubomír - Gelnar, Milan - Kašný, Martin
PY - 2016
TI - Cystatins of helminths: characterization of molecules with immunomodulatory potential
KW - Helminths
KW - Cystatin
KW - immunomodulation
KW - Bloodfeeding
N2 - Cystatins cysteine peptidase inhibitors are produced by a wide range of organisms, including parasites, where they act as modulators of the host immune system. In the frame of our research we focused on characterization of cystatins produced by four helminth species representing most important groups of helminth parasites, namely Eudiplozoon nipponicum (Monogenea), Fascioloides magna (Trematoda), Hymenolepis diminuta (Cestoda) and Trichinella spiralis (Nematoda). We performed a bacterial expression of optimized cystatin genes in recombinant forms (E. coli BL21 cells, pET32a and/or pET19b plasmid vector). The recombinant cystatins were purified via Histag using metal affinity chromatography (NiNTA) and successful production of cystatin was confirmed by mass spectrometric methods (MALDI TOF/TOF). Selected candidates were used for immunization of mice and cystatins were immunolocalized on cryosections of parasite tissues using positive mice sera, FITClabeled antimouse antibodies and DAPI counterstaining. The inhibitory effect of particular cystatins was fluorometrically measured by adoption of recombinant cysteine peptidases (mouse cathepsin L, cathepsin L3 from E. nipponicum) and its fluorogenic peptide substrate (FRAMC). The immunomodulatory potential of presented cystatins (modulation of antigen presentation, influence on cytokine production and proliferation of lymphocytes, macrophages, etc.) will be studied in detail.
ER -
ILGOVÁ, Jana, Lucie JEDLIČKOVÁ, Hana DVOŘÁKOVÁ, Libor MIKEŠ, Lubomír JANDA, Milan GELNAR a Martin KAŠNÝ. Cystatins of helminths: characterization of molecules with immunomodulatory potential. Online. In \textit{Europian Multicolloquium On Parasitology}. 2016, [citováno 2024-04-23]
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