Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.

ŠKORPÍKOVÁ, Lucie, Martin KAŠNÝ, Michal SLANÝ, Petr KRÁLÍK, Milan GELNAR a Jana ILGOVÁ. Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics. In 12. České a slovenské parazitologické dny. 2016.

Základní údaje

• Originální název: Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.
• Název česky: Cystatiny parazitických hlístic rodu Trichinella spiralis a T. pseudospiralis: xMAP technologie v serodiagnostice.
• Název anglicky: Cystatins of the parasitic nematodes Trichinella spiralis and T. pseudospiralis: xMAP technology in serodiagnostics.
• Autoři: ŠKORPÍKOVÁ, Lucie (203 Česká republika, domácí), Martin KAŠNÝ (203 Česká republika), Michal SLANÝ (203 Česká republika), Petr KRÁLÍK (203 Česká republika), Milan GELNAR (203 Česká republika) a Jana ILGOVÁ (703 Slovensko).
• Vydání: 12. České a slovenské parazitologické dny, 2016.

Další údaje

• Originální jazyk: čeština
• Typ výsledku: Prezentace na konferencích
• Obor: 10600 1.6 Biological sciences
• Stát vydavatele: Česká republika
• Utajení: není předmětem státního či obchodního tajemství
• Kód RIV: RIV/00216224:14310/16:00091092
• Organizační jednotka: Přírodovědecká fakulta
• Klíčová slova česky: cystatiny; Trichinella, serodiagnostika; potravinoví parazité
• Klíčová slova anglicky: cystatins; Trichinella; serodiagnstics; food-borne parasites

Anotace

Nematodes of the genus Trichinella are clinically important parasites invading cells of the small intestine and subsequently skeletal muscles. Their global distribution is corresponding to the wide range of their hosts involving birds, reptiles and mammals. Seven (T1, T2, T3, T4, T5, T6 a T10) of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious and sometimes fatal human disease. During the life cycle, the individuals of this nematode parasite release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. These molecules are essential for many biological processes like development, digestion, migration, and also in manipulation of the host immunne system. The most dominant components of the E-S products are cystatins – inhibitors of cysteine peptidases of C1 and rarely of C13 family. Our work is focused on the characterization of „multi cystatin-like domain“ (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared. The recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnosis of parasitic nematodes from the genus Trichinella. We employed the xMAP technology - novel diagnostic platform combining immunoassay approach and magnetic microspheres.

Anotace anglicky

Nematodes of the genus Trichinella are clinically important parasites invading cells of the small intestine and subsequently skeletal muscles. Their global distribution is corresponding to the wide range of their hosts involving birds, reptiles and mammals. Seven (T1, T2, T3, T4, T5, T6 a T10) of twelve genotypes of Trichinella spp. are zoonoses causing the trichinellosis - a serious and sometimes fatal human disease. During the life cycle, the individuals of this nematode parasite release variety of functional molecules, which are mainly present in excretory-secretory (E-S) products. These molecules are essential for many biological processes like development, digestion, migration, and also in manipulation of the host immunne system. The most dominant components of the E-S products are cystatins – inhibitors of cysteine peptidases of C1 and rarely of C13 family. Our work is focused on the characterization of „multi cystatin-like domain“ (mcd-1) genes and proteins of Trichinella spiralis and T. pseudospiralis. The complete sequences of genes were obtained and recombinant analogs of MCD-1 proteins were prepared. The recombinant MCD-1 molecules are used also in regard to develop the reliable molecular method useful for serological quantitative and qualitative diagnosis of parasitic nematodes from the genus Trichinella. We employed the xMAP technology - novel diagnostic platform combining immunoassay approach and magnetic microspheres.

Návaznosti

• MUNI/A/1325/2015, interní kód MU: Název: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Akronym: BIDA5)

Investor: Masarykova univerzita, Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty