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@article{1357366,
author = {Grabiec, Marta and Hříbková, Hana and Vařecha, Miroslav and Střítecká, Dana and Hampl, Aleš and Dvořák, Petr and Sun, YuhandMan},
article_location = {Amsterdam},
article_number = {2},
doi = {http://dx.doi.org/10.1016/j.scr.2016.08.012},
keywords = {Neural developmental modeling; Embryonic stem cells; Neural fate; Neurogenesis; FGF2 signaling; Neural stem cell niche},
language = {eng},
issn = {1873-5061},
journal = {Stem Cell Research},
title = {Stage-specific roles of FGF2 signaling in human neural development},
volume = {17},
year = {2016}
}
TY - JOUR
ID - 1357366
AU - Grabiec, Marta - Hříbková, Hana - Vařecha, Miroslav - Střítecká, Dana - Hampl, Aleš - Dvořák, Petr - Sun, Yuh-Man
PY - 2016
TI - Stage-specific roles of FGF2 signaling in human neural development
JF - Stem Cell Research
VL - 17
IS - 2
SP - 330-341
EP - 330-341
PB - Elsevier Science BV
SN - 18735061
KW - Neural developmental modeling
KW - Embryonic stem cells
KW - Neural fate
KW - Neurogenesis
KW - FGF2 signaling
KW - Neural stem cell niche
N2 - This study elucidated the stage-specific roles of FGF2 signaling during neural development using in-vitro human embryonic stem cell-based developmental modeling. We found that the dysregulation of FGF2 signaling prior to the onset of neural induction resulted in the malformation of neural rosettes (a neural tube-like structure), despite cells having undergone neural induction. The aberrant neural rosette formation may be attributed to the misplacement of ZO-1, which is a polarized tight junction protein and shown co-localized with FGF2/FGFR1 in the apical region of neural rosettes, subsequently led to abnormal neurogenesis. Moreover, the FGF2 signaling inhibition at the stage of neural rosettes caused a reduction in cell proliferation, an increase in numbers of cells with cell-cycle exit, and premature neurogenesis. These effects may be mediated by NUMB, to which expression was observed enriched in the apical region of neural rosettes after FGF2 signaling inhibition coinciding with the disappearance of PAX6+/Ki67+ neural stem cells and the emergence of MAP2+ neurons. Moreover, our results suggested that the hESC-based developmental system reserved a similar neural stem cell niche in vivo.
ER -
GRABIEC, Marta, Hana HŘÍBKOVÁ, Miroslav VAŘECHA, Dana STŘÍTECKÁ, Aleš HAMPL, Petr DVOŘÁK a Yuh-Man SUN. Stage-specific roles of FGF2 signaling in human neural development. \textit{Stem Cell Research}. Amsterdam: Elsevier Science BV, 2016, roč.~17, č.~2, s.~330-341. ISSN~1873-5061. Dostupné z: https://dx.doi.org/10.1016/j.scr.2016.08.012.
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