The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate
Cancer Therapy

J 2016

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

MILOSAVLJEVIC, Vedran; Yazan HADDAD; Miguel Angel Merlos RODRIGO; Amitava MOULICK; Hana POLANSKÁ et. al.

Základní údaje

Originální název

The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy

Autoři

MILOSAVLJEVIC, Vedran (203 Česká republika); Yazan HADDAD (203 Česká republika); Miguel Angel Merlos RODRIGO (203 Česká republika); Amitava MOULICK (203 Česká republika); Hana POLANSKÁ (203 Česká republika, garant, domácí); David HYNEK (203 Česká republika); Zbynek HEGER (203 Česká republika); Pavel KOPEL (203 Česká republika) a Vojtech ADAM (203 Česká republika)

Vydání

Plos one, San Francisco, Public Library of Science, 2016, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.806

Kód RIV

RIV/00216224:14110/16:00092236

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1371/journal.pone.0163983

UT WoS

000385504400015

EID Scopus

2-s2.0-84991498330

Klíčová slova anglicky

TRANSCRIPTION FACTOR SP1; STEADY-STATE; CELL-LINES; EXPRESSION; APOPTOSIS; ACTIVATION; MICROARRAY; PROTEIN; MODEL; P53

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 10. 1. 2017 10:42, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MILOSAVLJEVIC, Vedran; Yazan HADDAD; Miguel Angel Merlos RODRIGO; Amitava MOULICK; Hana POLANSKÁ; David HYNEK; Zbynek HEGER; Pavel KOPEL a Vojtech ADAM. The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 10, s. 1-19. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0163983.

@article{1364186,
   author = {Milosavljevic, Vedran and Haddad, Yazan and Rodrigo, Miguel Angel Merlos and Moulick, Amitava and Polanská, Hana and Hynek, David and Heger, Zbynek and Kopel, Pavel and Adam, Vojtech},
   article_location = {San Francisco},
   article_number = {10},
   doi = {http://dx.doi.org/10.1371/journal.pone.0163983},
   keywords = {TRANSCRIPTION FACTOR SP1; STEADY-STATE; CELL-LINES; EXPRESSION; APOPTOSIS; ACTIVATION; MICROARRAY; PROTEIN; MODEL; P53},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy},
   volume = {11},
   year = {2016}
}

TY  - JOUR
ID  - 1364186
AU  - Milosavljevic, Vedran - Haddad, Yazan - Rodrigo, Miguel Angel Merlos - Moulick, Amitava - Polanská, Hana - Hynek, David - Heger, Zbynek - Kopel, Pavel - Adam, Vojtech
PY  - 2016
TI  - The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy
JF  - Plos one
VL  - 11
IS  - 10
SP  - 1-19
EP  - 1-19
PB  - Public Library of Science
SN  - 19326203
KW  - TRANSCRIPTION FACTOR SP1
KW  - STEADY-STATE
KW  - CELL-LINES
KW  - EXPRESSION
KW  - APOPTOSIS
KW  - ACTIVATION
KW  - MICROARRAY
KW  - PROTEIN
KW  - MODEL
KW  - P53
N2  - Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.
ER  -

MILOSAVLJEVIC, Vedran; Yazan HADDAD; Miguel Angel Merlos RODRIGO; Amitava MOULICK; Hana POLANSKÁ; David HYNEK; Zbynek HEGER; Pavel KOPEL a Vojtech ADAM. The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~10, s.~1-19. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0163983.

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