Monogenea: Parasites of fish and their unknown molecules

k 2017

Monogenea: Parasites of fish and their unknown molecules

VOREL, Jiří; Pavel ROUDNICKÝ; Milan GELNAR a Martin KAŠNÝ

Základní údaje

Originální název

Monogenea: Parasites of fish and their unknown molecules

Autoři

VOREL, Jiří; Pavel ROUDNICKÝ; Milan GELNAR a Martin KAŠNÝ

Vydání

Veterinary Vaccinology Network Conference 2017, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00094614

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Monogenea; Eudiplozoon; nipponicum; serpin; cystatin; excretory; secretory; prediction; vaccine

Změněno: 24. 3. 2017 15:24, RNDr. Martin Kašný, Ph.D.

Anotace

V originále

The majority of known monogenean species are ectoparasites of fish with monoxenous life cycle in freshwater or marine ecosystems. Some species are of high economic importance, they can be highly pathogenic to fish, which can result in losses in aquaculture. In contrast to these facts there is almost nothing known about the molecular nature of the interactions with fish hosts and functions of particular monogeneans bioactive molecules. In a first part of our pilot study of monogeneans molecules we adopted the NGS techniques in order to get the high quality “monogenean-polyopisthocotylean genome/transcriptome matrix”. In a second part of our study this platform was used for the identification of the monogeneans´ dominant protein molecules followed by their further molecular/biochemical characterization. We adopted experimental organism Eudiplozoon nipponicum (Platyhelminthes, Monogenea) in order to obtain high quality genome, transcriptome and proteome for further annotation, development of robust gene/protein muster platform for this group of organisms. Subsequently, we used this dataset in combination with bioinformatics approach enable in silico determination of antigenic proteins - vaccine candidates. In addition to the bioinformatics analyses we performed mass spectrometric identification of particular protein compounds in excretory-secretory products from E. nipponicum in order to confirm the presence of previously predicted antigenic molecules. Finally, some of the predicted molecules have been prepared in recombinant form and characterized (serpins, cystatins).

Návaznosti

GAP506/12/1258, projekt VaV

Název: Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

Investor: Grantová agentura ČR, Interakce hostitel-parazit u krevsajících diplozoidních monogeneí: Výzkum vysoce specializovaných adaptací k parazitismu

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

VOREL, Jiří; Pavel ROUDNICKÝ; Milan GELNAR a Martin KAŠNÝ. Monogenea: Parasites of fish and their unknown molecules. In Veterinary Vaccinology Network Conference 2017. 2017.

@proceedings{1368621,
   author = {Vorel, Jiří and Roudnický, Pavel and Gelnar, Milan and Kašný, Martin},
   booktitle = {Veterinary Vaccinology Network Conference 2017},
   keywords = {Monogenea; Eudiplozoon; nipponicum; serpin; cystatin; excretory; secretory; prediction; vaccine},
   language = {eng},
   title = {Monogenea: Parasites of fish and their unknown molecules},
   year = {2017}
}

TY  - CONF
ID  - 1368621
AU  - Vorel, Jiří - Roudnický, Pavel - Gelnar, Milan - Kašný, Martin
PY  - 2017
TI  - Monogenea: Parasites of fish and their unknown molecules
KW  - Monogenea
KW  - Eudiplozoon
KW  - nipponicum
KW  - serpin
KW  - cystatin
KW  - excretory
KW  - secretory
KW  - prediction
KW  - vaccine
N2  - The majority of known monogenean species are ectoparasites of fish with monoxenous life cycle in freshwater or marine ecosystems. Some species are of high economic importance, they can be highly pathogenic to fish, which can result in losses in aquaculture. In contrast to these facts there is almost nothing known about the molecular nature of the interactions with fish hosts and functions of particular monogeneans bioactive molecules. In a first part of our pilot study of monogeneans molecules we adopted the NGS techniques in order to get the high quality “monogenean-polyopisthocotylean genome/transcriptome matrix”. In a second part of our study this platform was used for the identification of the monogeneans´ dominant protein molecules followed by their further molecular/biochemical characterization. We adopted experimental organism Eudiplozoon nipponicum (Platyhelminthes, Monogenea) in order to obtain high quality genome, transcriptome and proteome for further annotation, development of robust gene/protein muster platform for this group of organisms. Subsequently, we used this dataset in combination with bioinformatics approach enable in silico determination of antigenic proteins - vaccine candidates. In addition to the bioinformatics analyses we performed mass spectrometric identification of particular protein compounds in excretory-secretory products from E. nipponicum in order to confirm the presence of previously predicted antigenic molecules. Finally, some of the predicted molecules have been prepared in recombinant form and characterized (serpins, cystatins).
ER  -

VOREL, Jiří; Pavel ROUDNICKÝ; Milan GELNAR a Martin KAŠNÝ. Monogenea: Parasites of fish and their unknown molecules. In \textit{Veterinary Vaccinology Network Conference 2017}. 2017.

Přehled o publikaci