Early and progressive microstructural brain changes in mice
overexpressing human alpha-Synuclein detected by diffusion
kurtosis imaging

J 2017

Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging

KHAIRNAR, Amit Suresh; Jana RUDÁ; Nikoletta SZABÓ; Eva DRAŽANOVÁ; Anas ARAB et al.

Základní údaje

Originální název

Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging

Autoři

KHAIRNAR, Amit Suresh; Jana RUDÁ; Nikoletta SZABÓ; Eva DRAŽANOVÁ; Anas ARAB; Birgit HUTTER-PAIER; Joerg NEDDENS; Peter LATTA; Zenon STARČUK a Irena REKTOROVÁ

Vydání

Brain, Behavior, and Immunity, San Diego, Academic Press, 2017, 0889-1591

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.306

Kód RIV

RIV/00216224:14740/17:00096844

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1016/j.bbi.2016.11.027

UT WoS

000395365900022

EID Scopus

2-s2.0-85010773884

Klíčová slova anglicky

MRI; Diffusion kurtosis imaging; Substantia nigra; Striatum; Thalamus; TNWT-61; Parkinson's disease; Transgenic mice; Animal model

Štítky

CF MAFIL, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 2. 2018 11:11, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Diffusion kurtosis imaging (DKI) is sensitive in detecting alpha-Synuclein (alpha-Syn) accumulation-associated microstructural changes at late stages of the pathology in alpha-Syn overexpressing TNWT-61 mice. The aim of this study was to perform DKI in young TNWT-61 mice when alpha-Syn starts to accumulate and to compare the imaging results with an analysis of motor and memory impairment and alpha-Syn levels. Three-month-old (3mo) and six-month-old (6mo) mice underwent DIU scanning using the Bruker Avance 9.4 T magnetic resonance imaging system. Region of interest (ROI) analyses were performed in the gray matter; tract-based spatial statistics (TBSS) analyses were performed in the white matter. In the same mice, alpha-Syn expression was evaluated using quantitative immunofluorescence. Mean kurtosis (MK) was the best differentiator between TNWT-61 mice and wildtype (WT) mice. We found increases in MK in 3mo TNWT-61 mice in the striatum and thalamus but not in the substantia nigra (SN), hippocampus, or sensorimotor cortex, even though the immunoreactivity of human alpha-Syn was similar or even higher in the latter regions. Increases in MK in the SN were detected in 6mo mice. These findings indicate that alpha-Syn accumulation-associated changes may start in areas with a high density of dopaminergic nerve terminals. We also found TBSS changes in white matter only at 6mo, suggesting alpha-Syn accumulation-associated changes start in the gray matter and later progress to the white matter. MK is able to detect microstructural changes induced by alpha-Syn overexpression in TNWT-61 mice and could be a useful clinical tool for detecting early-stage Parkinson's disease in human patients. (C) 2016 Elsevier Inc. All rights reserved.

Návaznosti

LM2015062, projekt VaV

Název: Národní infrastruktura pro biologické a medicínské zobrazování

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, National research infrastructure for biological and medical imaging

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/A/1063/2016, interní kód MU

Název: Experimentální a translační farmakologický výzkum a vývoj

Investor: Masarykova univerzita, Experimentální a translační farmakologický výzkum a vývoj, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

KHAIRNAR, Amit Suresh; Jana RUDÁ; Nikoletta SZABÓ; Eva DRAŽANOVÁ; Anas ARAB; Birgit HUTTER-PAIER; Joerg NEDDENS; Peter LATTA; Zenon STARČUK a Irena REKTOROVÁ. Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging. Brain, Behavior, and Immunity. San Diego: Academic Press, 2017, roč. 61, March, s. 197-208. ISSN 0889-1591. Dostupné z: https://doi.org/10.1016/j.bbi.2016.11.027.

@article{1381942,
   author = {Khairnar, Amit Suresh and Rudá, Jana and Szabó, Nikoletta and Dražanová, Eva and Arab, Anas and HutterandPaier, Birgit and Neddens, Joerg and Latta, Peter and Starčuk, Zenon and Rektorová, Irena},
   article_location = {San Diego},
   article_number = {March},
   doi = {https://doi.org/10.1016/j.bbi.2016.11.027},
   keywords = {MRI; Diffusion kurtosis imaging; Substantia nigra; Striatum; Thalamus; TNWT-61; Parkinson's disease; Transgenic mice; Animal model},
   language = {eng},
   issn = {0889-1591},
   journal = {Brain, Behavior, and Immunity},
   title = {Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging},
   url = {http://www.sciencedirect.com/science/article/pii/S0889159116305293},
   volume = {61},
   year = {2017}
}

TY  - JOUR
ID  - 1381942
AU  - Khairnar, Amit Suresh - Rudá, Jana - Szabó, Nikoletta - Dražanová, Eva - Arab, Anas - Hutter-Paier, Birgit - Neddens, Joerg - Latta, Peter - Starčuk, Zenon - Rektorová, Irena
PY  - 2017
TI  - Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging
JF  - Brain, Behavior, and Immunity
VL  - 61
IS  - March
SP  - 197-208
EP  - 197-208
PB  - Academic Press
SN  - 08891591
KW  - MRI
KW  - Diffusion kurtosis imaging
KW  - Substantia nigra
KW  - Striatum
KW  - Thalamus
KW  - TNWT-61
KW  - Parkinson's disease
KW  - Transgenic mice
KW  - Animal model
UR  - http://www.sciencedirect.com/science/article/pii/S0889159116305293
L2  - http://www.sciencedirect.com/science/article/pii/S0889159116305293
N2  - Diffusion kurtosis imaging (DKI) is sensitive in detecting alpha-Synuclein (alpha-Syn) accumulation-associated microstructural changes at late stages of the pathology in alpha-Syn overexpressing TNWT-61 mice. The aim of this study was to perform DKI in young TNWT-61 mice when alpha-Syn starts to accumulate and to compare the imaging results with an analysis of motor and memory impairment and alpha-Syn levels. Three-month-old (3mo) and six-month-old (6mo) mice underwent DIU scanning using the Bruker Avance 9.4 T magnetic resonance imaging system. Region of interest (ROI) analyses were performed in the gray matter; tract-based spatial statistics (TBSS) analyses were performed in the white matter. In the same mice, alpha-Syn expression was evaluated using quantitative immunofluorescence. Mean kurtosis (MK) was the best differentiator between TNWT-61 mice and wildtype (WT) mice. We found increases in MK in 3mo TNWT-61 mice in the striatum and thalamus but not in the substantia nigra (SN), hippocampus, or sensorimotor cortex, even though the immunoreactivity of human alpha-Syn was similar or even higher in the latter regions. Increases in MK in the SN were detected in 6mo mice. These findings indicate that alpha-Syn accumulation-associated changes may start in areas with a high density of dopaminergic nerve terminals. We also found TBSS changes in white matter only at 6mo, suggesting alpha-Syn accumulation-associated changes start in the gray matter and later progress to the white matter. MK is able to detect microstructural changes induced by alpha-Syn overexpression in TNWT-61 mice and could be a useful clinical tool for detecting early-stage Parkinson's disease in human patients. (C) 2016 Elsevier Inc. All rights reserved.
ER  -

KHAIRNAR, Amit Suresh; Jana RUDÁ; Nikoletta SZABÓ; Eva DRAŽANOVÁ; Anas ARAB; Birgit HUTTER-PAIER; Joerg NEDDENS; Peter LATTA; Zenon STARČUK a Irena REKTOROVÁ. Early and progressive microstructural brain changes in mice overexpressing human alpha-Synuclein detected by diffusion kurtosis imaging. \textit{Brain, Behavior, and Immunity}. San Diego: Academic Press, 2017, roč.~61, March, s.~197-208. ISSN~0889-1591. Dostupné z: https://doi.org/10.1016/j.bbi.2016.11.027.

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