Microscopic anatomy of monogenean parasite Paradiplozoon
homoion

a 2017

Microscopic anatomy of monogenean parasite Paradiplozoon homoion

HODOVÁ, Iveta; Radim SONNEK; Milan GELNAR a Andrea BARDŮNEK VALIGUROVÁ

Základní údaje

Originální název

Microscopic anatomy of monogenean parasite Paradiplozoon homoion

Autoři

HODOVÁ, Iveta; Radim SONNEK; Milan GELNAR a Andrea BARDŮNEK VALIGUROVÁ

Vydání

8th International Symposium on Monogenea, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00094884

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8665-4

Klíčová slova anglicky

Diplozoidae; confocal microscopy; electron microscopy; anatomy

Příznaky

Mezinárodní význam

Změněno: 14. 8. 2017 10:09, Mgr. Iveta Hodová, Ph.D.

Anotace

V originále

The species Paradiplozoon homoion (Bychowsky et Nagibina, 1959) belongs to the family Diplozoidae (Monogenea), representing blood-feeding gill ectoparasites of freshwater fish. Diplozoids exhibit extraordinary morphology and life cycle involving permanent fusion of two larval worms and their subsequent transformation into one individual. Samples of P. homoion were collected from the gills of roach (Rutilus rutilus L.), bleak (Alburnus alburnus L.) and gudgeon (Gobio gobio L.) caught by electrofishing or seine netting in the Mušov reservoir (southern Moravia, Czech Republic). Using a combined approach of confocal and electron microscopy, adults of monogenean parasite P. homoion were investigated for the body organisation and adaptation to the ectoparasitic lifestyle, with emphasis on worm’s forebody and hindbody. Special attention was given to the parasite’s tegument, digestive, reproductive, and excretory systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

HODOVÁ, Iveta; Radim SONNEK; Milan GELNAR a Andrea BARDŮNEK VALIGUROVÁ. Microscopic anatomy of monogenean parasite Paradiplozoon homoion. In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8665-4.

@proceedings{1387266,
   author = {Hodová, Iveta and Sonnek, Radim and Gelnar, Milan and Bardůnek Valigurová, Andrea},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {Diplozoidae; confocal microscopy; electron microscopy; anatomy},
   language = {eng},
   isbn = {978-80-210-8665-4},
   title = {Microscopic anatomy of monogenean parasite Paradiplozoon homoion},
   year = {2017}
}

TY  - CONF
ID  - 1387266
AU  - Hodová, Iveta - Sonnek, Radim - Gelnar, Milan - Bardůnek Valigurová, Andrea
PY  - 2017
TI  - Microscopic anatomy of monogenean parasite Paradiplozoon homoion
SN  - 9788021086654
KW  - Diplozoidae
KW  - confocal microscopy
KW  - electron microscopy
KW  - anatomy
N2  - The species Paradiplozoon homoion (Bychowsky et Nagibina, 1959) belongs to the family Diplozoidae (Monogenea), representing blood-feeding gill ectoparasites of freshwater fish. Diplozoids exhibit extraordinary morphology and life cycle involving permanent fusion of two larval worms and their subsequent transformation into one individual. Samples of P. homoion were collected from the gills of roach (Rutilus rutilus L.), bleak (Alburnus alburnus L.) and gudgeon (Gobio gobio L.) caught by electrofishing or seine netting in the Mušov reservoir (southern Moravia, Czech Republic). Using a combined approach of confocal and electron microscopy, adults of monogenean parasite P. homoion were investigated for the body organisation and adaptation to the ectoparasitic lifestyle, with emphasis on worm’s forebody and hindbody. Special attention was given to the parasite’s tegument, digestive, reproductive, and excretory systems. Labelling of filamentous actin with fluorescent phalloidin revealed the musculature of the body wall, buccal suckers, the pharynx as well as some parts of reproductive tract. Staining with chloride carmine and counterstaining with DAPI proved to be powerful tools for confocal microscopic detection of various cell types including putative gland cells. Immunofluorescent detection of alfa-tubulin showed to be suitable for visualisation of excretory system and some parts of nervous system.
ER  -

HODOVÁ, Iveta; Radim SONNEK; Milan GELNAR a Andrea BARDŮNEK VALIGUROVÁ. Microscopic anatomy of monogenean parasite Paradiplozoon homoion. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8665-4.

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