Serpin from Eudiplozoon nipponicum

a 2017

Serpin from Eudiplozoon nipponicum

ROUDNICKÝ, Pavel; Jiří VOREL; Jana ILGOVÁ; Libor MIKEŠ; Lucie JEDLIČKOVÁ et al.

Základní údaje

Originální název

Serpin from Eudiplozoon nipponicum

Autoři

ROUDNICKÝ, Pavel; Jiří VOREL; Jana ILGOVÁ; Libor MIKEŠ; Lucie JEDLIČKOVÁ; John DALTON; Jan DVOŘÁK; Lubomír JANDA; Adam NOREK; Milan GELNAR a Martin KAŠNÝ

Vydání

8th International Symposium on Monogenea, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

ECIP - European Centre of IchthyoParasitology

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00094922

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8666-1

Klíčová slova anglicky

serpin; parasite; eudiplozoon; interaction host-parasite; inhibition

Změněno: 11. 9. 2017 15:48, Mgr. Pavel Roudnický, Ph.D.

Anotace

V originále

The properties of proteins (e.g. functions) of the members from the family Monogenea are among the less investigated in whole phylum Platyhelminthes. We chose Eudiplozoon nipponicum as our experimental organism to address this issue and complete the mosaic of its functional proteins equipment. E. nipponicum (family Diplozoidae, Polyopisthocotylea) is hematophagous ectoparasite which lives on the gills of common carp (Cyprinus carpio). The main aim of our current work is to understand the regulation of peptidase activity related to many physiological processes. Among the key regulatory factors could be included the peptidase inhibitors, such as serpins - serine peptidase inhibitors. These functional proteins are generally known as important regulators of the coagulation cascade, complement, fibrinolysis, angiogenesis, inflammation etc. The inhibition, mediated by serpins, is typically irreversible, comprising conformational changes in serpin molecule leading to distortion of peptidase tertiary structure. Except the inhibition, some of them may have other functions like e.g. protein transporters or chaperones and they are relatively abundant also in secretions, body lysates and genomes/transcriptomes of helminths. We identified serpin gene/protein in transcriptome of E. nipponicum and investigated properties of its recombinant form – molecular and biochemical characterization was performed. The tertiary structure was predicted, antigenic properties evaluated and inhibitory effect measured.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

ROUDNICKÝ, Pavel; Jiří VOREL; Jana ILGOVÁ; Libor MIKEŠ; Lucie JEDLIČKOVÁ; John DALTON; Jan DVOŘÁK; Lubomír JANDA; Adam NOREK; Milan GELNAR a Martin KAŠNÝ. Serpin from Eudiplozoon nipponicum. In 8th International Symposium on Monogenea. 2017. ISBN 978-80-210-8666-1.

@proceedings{1389088,
   author = {Roudnický, Pavel and Vorel, Jiří and Ilgová, Jana and Mikeš, Libor and Jedličková, Lucie and Dalton, John and Dvořák, Jan and Janda, Lubomír and Norek, Adam and Gelnar, Milan and Kašný, Martin},
   booktitle = {8th International Symposium on Monogenea},
   keywords = {serpin; parasite; eudiplozoon; interaction host-parasite; inhibition},
   language = {eng},
   isbn = {978-80-210-8666-1},
   title = {Serpin from Eudiplozoon nipponicum},
   url = {http://ecip.cz/news/4},
   year = {2017}
}

TY  - CONF
ID  - 1389088
AU  - Roudnický, Pavel - Vorel, Jiří - Ilgová, Jana - Mikeš, Libor - Jedličková, Lucie - Dalton, John - Dvořák, Jan - Janda, Lubomír - Norek, Adam - Gelnar, Milan - Kašný, Martin
PY  - 2017
TI  - Serpin from Eudiplozoon nipponicum
SN  - 9788021086661
KW  - serpin
KW  - parasite
KW  - eudiplozoon
KW  - interaction host-parasite
KW  - inhibition
UR  - http://ecip.cz/news/4
L2  - http://ecip.cz/news/4
N2  - The properties of proteins (e.g. functions) of the members from the family Monogenea are among the less investigated in whole phylum Platyhelminthes. We chose Eudiplozoon nipponicum as our experimental organism to address this issue and complete the mosaic of its functional proteins equipment. E. nipponicum (family Diplozoidae, Polyopisthocotylea) is hematophagous ectoparasite which lives on the gills of common carp (Cyprinus carpio). The main aim of our current work is to understand the regulation of peptidase activity related to many physiological processes. Among the key regulatory factors could be included the peptidase inhibitors, such as serpins - serine peptidase inhibitors. These functional proteins are generally known as important regulators of the coagulation cascade, complement, fibrinolysis, angiogenesis, inflammation etc. The inhibition, mediated by serpins, is typically irreversible, comprising conformational changes in serpin molecule leading to distortion of peptidase tertiary structure. Except the inhibition, some of them may have other functions like e.g. protein transporters or chaperones and they are relatively abundant also in secretions, body lysates and genomes/transcriptomes of helminths. We identified serpin gene/protein in transcriptome of E. nipponicum and investigated properties of its recombinant form – molecular and biochemical characterization was performed. The tertiary structure was predicted, antigenic properties evaluated and inhibitory effect measured.
ER  -

ROUDNICKÝ, Pavel; Jiří VOREL; Jana ILGOVÁ; Libor MIKEŠ; Lucie JEDLIČKOVÁ; John DALTON; Jan DVOŘÁK; Lubomír JANDA; Adam NOREK; Milan GELNAR a Martin KAŠNÝ. Serpin from Eudiplozoon nipponicum. In \textit{8th International Symposium on Monogenea}. 2017. ISBN~978-80-210-8666-1.

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