Myeloperoxidase (MPO) gene variability in patients with
recurrent aphthous stomatitis

a 2017

Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis

MASOPUSTOVÁ, Lucie; Petra BOŘILOVÁ LINHARTOVÁ; Simona SLEZÁKOVÁ; Jiřina BÁRTOVÁ; Jitka PETANOVÁ et al.

Základní údaje

Originální název

Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis

Autoři

MASOPUSTOVÁ, Lucie; Petra BOŘILOVÁ LINHARTOVÁ; Simona SLEZÁKOVÁ; Jiřina BÁRTOVÁ; Jitka PETANOVÁ; Pavel KUKLÍNEK; Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ

Vydání

XVIII. Setkání biochemiků a molekulárních biologů, Brno, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30208 Dentistry, oral surgery and medicine

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/17:00100749

Organizační jednotka

Lékařská fakulta

ISBN

978-80-210-8765-1

Klíčová slova anglicky

myeloperoxidase; association studies; candidate gene; genetic; immunity; oral ulcer; polymorphisms

Změněno: 18. 1. 2019 14:06, Mgr. et Mgr. Simona Slezáková, Ph.D.

Anotace

V originále

Introduction: Recurrent aphthous stomatitis (RAS) is a multifactorial disease, the exact etiological factor(s) are not understood. One of the contributing factors is oxidative stress caused by an imbalance between reactive oxygen species (ROS) and an antioxidant system. Myeloperoxidase (MPO) is an enzyme utilizing the oxidation potential of superoxides and hydrogen peroxide to convert chloride ions to chlorine and another ROS. The aim of this study was to determine polymorphisms in MPO (rs2243828, rs2759, rs7208693, rs2333227) in RAS patients and control subjects in Czech population. Materials and methods: We performed a case–control study in 275 subjects (70 patients with recurrent aphthous stomatitis and 205 healthy individuals). Polymorphisms were detected by using real-time PCR with the use of TaqMan probe and RFLP-PCR with restriction analysis. Results: No statistically significant differences were found in allelic and genotypic frequencies of MPO polymorphisms (rs2243828, rs2759, rs7208693, rs2333227) among patients with RAS and controls. However statistical significance was found in SNP MPO (rs2333227, rs2243828) when comparing the duration of lesions, difference is between individuals with the GG genotype compared to carriers of AA + AG genotypes (P = 0.02). No significant association was detected between MPO haplotypes and RAS. Conclusion: When comparing our results with studies in the Turkish population, we concluded that variability in the myeloperoxidase gene may not play a role in the development of the disease. Acknowledgements: This study was supported by grant AZV 15-29336A, GAČR GB14-37368G and project MUNI / A / 0948/2016.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

MUNI/A/0948/2016, interní kód MU

Název: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba

Investor: Masarykova univerzita, Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

MASOPUSTOVÁ, Lucie; Petra BOŘILOVÁ LINHARTOVÁ; Simona SLEZÁKOVÁ; Jiřina BÁRTOVÁ; Jitka PETANOVÁ; Pavel KUKLÍNEK; Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis. In XVIII. Setkání biochemiků a molekulárních biologů, Brno. 2017. ISBN 978-80-210-8765-1.

@proceedings{1395667,
   author = {Masopustová, Lucie and Bořilová Linhartová, Petra and Slezáková, Simona and Bártová, Jiřina and Petanová, Jitka and Kuklínek, Pavel and Fassmann, Antonín and Izakovičová Hollá, Lydie},
   booktitle = {XVIII. Setkání biochemiků a molekulárních biologů, Brno},
   keywords = {myeloperoxidase; association studies; candidate gene; genetic; immunity; oral ulcer; polymorphisms},
   language = {eng},
   isbn = {978-80-210-8765-1},
   title = {Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis},
   year = {2017}
}

TY  - CONF
ID  - 1395667
AU  - Masopustová, Lucie - Bořilová Linhartová, Petra - Slezáková, Simona - Bártová, Jiřina - Petanová, Jitka - Kuklínek, Pavel - Fassmann, Antonín - Izakovičová Hollá, Lydie
PY  - 2017
TI  - Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis
SN  - 9788021087651
KW  - myeloperoxidase
KW  - association studies
KW  - candidate gene
KW  - genetic
KW  - immunity
KW  - oral ulcer
KW  - polymorphisms
N2  - Introduction: Recurrent aphthous stomatitis (RAS) is a multifactorial disease, the exact etiological factor(s) are not understood. One of the contributing factors is oxidative stress caused by an imbalance between reactive oxygen species (ROS) and an antioxidant system. Myeloperoxidase (MPO) is an enzyme utilizing the oxidation potential of superoxides and hydrogen peroxide to convert chloride ions to chlorine and another ROS. The aim of this study was to determine polymorphisms in MPO (rs2243828, rs2759, rs7208693, rs2333227) in RAS patients and control subjects in Czech population. Materials and methods: We performed a case–control study in 275 subjects (70 patients with recurrent aphthous stomatitis and 205 healthy individuals). Polymorphisms were detected by using real-time PCR with the use of TaqMan probe and RFLP-PCR with restriction analysis. Results: No statistically significant differences were found in allelic and genotypic frequencies of MPO polymorphisms (rs2243828, rs2759, rs7208693, rs2333227) among patients with RAS and controls. However statistical significance was found in SNP MPO (rs2333227, rs2243828) when comparing the duration of lesions, difference is between individuals with the GG genotype compared to carriers of AA + AG genotypes (P = 0.02). No significant association was detected between MPO haplotypes and RAS. Conclusion: When comparing our results with studies in the Turkish population, we concluded that variability in the myeloperoxidase gene may not play a role in the development of the disease. Acknowledgements: This study was supported by grant AZV 15-29336A, GAČR GB14-37368G and project MUNI / A / 0948/2016.
ER  -

MASOPUSTOVÁ, Lucie; Petra BOŘILOVÁ LINHARTOVÁ; Simona SLEZÁKOVÁ; Jiřina BÁRTOVÁ; Jitka PETANOVÁ; Pavel KUKLÍNEK; Antonín FASSMANN a Lydie IZAKOVIČOVÁ HOLLÁ. Myeloperoxidase (MPO) gene variability in patients with recurrent aphthous stomatitis. In \textit{XVIII. Setkání biochemiků a molekulárních biologů, Brno}. 2017. ISBN~978-80-210-8765-1.

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