Purinergic receptor (P2RX7) gene variability in Czech patients
with diabetes mellitus type 2 and/or chronic periodontitis

a 2017

Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis

JÁNOŠ, Július, Petra BOŘILOVÁ LINHARTOVÁ, Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ

Základní údaje

Originální název

Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis

Autoři

JÁNOŠ, Július, Petra BOŘILOVÁ LINHARTOVÁ, Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ

Vydání

XVIII. Setkání biochemiků a molekulárních biologů, Brno, 2017

Další údaje

Typ výsledku

Konferenční abstrakt

Utajení

není předmětem státního či obchodního tajemství

ISBN

978-80-210-8765-1

Klíčová slova česky

purinergní receptor; diabetes mellitus; parodontitida; chronický zápal; genová variabilita; polymorfismus

Klíčová slova anglicky

purinergic receptor; diabetes mellitus; periodontitis; chronic inflammation; gene variability; polymorphism

Změněno: 19. 11. 2017 14:29, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Anotace

V originále

Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P<0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation. Acknowledgements: The study was supported by funds provided by the Faculty of Medicine MU to junior researcher Petra Borilova Linhartova, grant GACR GB14-37368G and project MUNI/A/0948/2016.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

MUNI/A/0948/2016, interní kód MU

Název: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba

Investor: Masarykova univerzita, Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/25/LF/2017, interní kód MU

Název: LF - Příspěvek na IP 2017

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2017, Interní rozvojové projekty

Citovat

JÁNOŠ, Július, Petra BOŘILOVÁ LINHARTOVÁ, Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ. Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis. In XVIII. Setkání biochemiků a molekulárních biologů, Brno. 2017. ISBN 978-80-210-8765-1.

@proceedings{1395730,
   author = {Jánoš, Július and Bořilová Linhartová, Petra and Poskerová, Hana and Izakovičová Hollá, Lydie},
   booktitle = {XVIII. Setkání biochemiků a molekulárních biologů, Brno},
   keywords = {purinergic receptor; diabetes mellitus; periodontitis; chronic inflammation; gene variability; polymorphism},
   isbn = {978-80-210-8765-1},
   title = {Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis},
   year = {2017}
}

TY  - CONF
ID  - 1395730
AU  - Jánoš, Július - Bořilová Linhartová, Petra - Poskerová, Hana - Izakovičová Hollá, Lydie
PY  - 2017
TI  - Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis
SN  - 9788021087651
KW  - purinergic receptor
KW  - diabetes mellitus
KW  - periodontitis
KW  - chronic inflammation
KW  - gene variability
KW  - polymorphism
N2  - Background: A bidirectional relationship between chronic periodontitis (CP) and diabetes mellitus type 2 (T2DM), characterized by low-grade inflammation, was previously described. Purinergic signaling plays a role in the activation of multiprotein intracellular complexes called inflammasomes, which control release of potent proinflammatory cytokines. The aim of the present study was to analyze two purinergic receptor (P2RX7) gene variants with gain-of-function effect in patients T2DM and/or CP in Czech population. Subjects and methods: Totally, 473 unrelated subjects were included in this case-control study. Genomic DNA of 208 patients with CP, 83 patients with T2DM+CP and 182 systemically healthy non-periodontitis controls were genotyped using the qPCR TaqMan method for His155Tyr (rs208294, C/T) and Ala348Thr (rs1718119, A/G) polymorphisms in the P2RX7 gene. Results: No significant differences in allele and/or genotype frequencies of P2RX7 His155Tyr between cases and controls were found. However, the G allele and GG genotype of P2RX7 Ala348Thr variant were marginally associated with CP (P=0.065 and P=0.079, respectively). In addition, the GG genotype, encoding Ala/Ala in amino acid sequence, was negatively correlated with levels of glycated hemoglobin (HbA1c) in T2DM patients (P<0.01); patients with Thr/Thr genotype that is associated with gain-of-function had the highest levels of HbA1c. Conclusions: Although only marginal association of polymorphism P2RX7 Ala348Thr with susceptibility to CP in the Czech population was found, purinergic signaling via P2RX7 gene variability might influence glucose regulation. Acknowledgements: The study was supported by funds provided by the Faculty of Medicine MU to junior researcher Petra Borilova Linhartova, grant GACR GB14-37368G and project MUNI/A/0948/2016.
ER  -

JÁNOŠ, Július, Petra BOŘILOVÁ LINHARTOVÁ, Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ. Purinergic receptor (P2RX7) gene variability in Czech patients with diabetes mellitus type 2 and/or chronic periodontitis. In \textit{XVIII. Setkání biochemiků a molekulárních biologů, Brno}. 2017. ISBN~978-80-210-8765-1.

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