IBRAHIM, Emad, Pavel DOBEŠ, Martin KUNC, Pavel HYRŠL a Dalibor KODRÍK. Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster. Journal of Insect Physiology, Kidlington, England: Elsevier, 2018, roč. 107, č. 4, s. 167-174. ISSN 0022-1910. doi:10.1016/j.jinsphys.2018.04.002.
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Základní údaje
Originální název Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
Název česky Adipokinetic hormone and adenosine interfere with nematobacterial infection and locomotion in Drosophila melanogaster
Autoři IBRAHIM, Emad (818 Egypt), Pavel DOBEŠ (203 Česká republika, domácí), Martin KUNC (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, garant, domácí) a Dalibor KODRÍK (203 Česká republika).
Vydání Journal of Insect Physiology, Kidlington, England, Elsevier, 2018, 0022-1910.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor Physiology
Stát vydavatele Spojené království
Utajení není předmětem státního či obchodního tajemství
Impakt faktor Impact factor: 2.733 v roce 2017
Kód RIV RIV/00216224:14310/18:00100913
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.1016/j.jinsphys.2018.04.002
UT WoS 000434751100021
Klíčová slova česky Adipokinetický hormon; adenosin; Drosophila; hlístice; oxidativní stres; lokomoce
Klíčová slova anglicky Adipokinetic hormone; adenosine; Drosophila; nematode; oxidative stress; locomotion
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Lucie Nesvadbová, učo 394502. Změněno: 15. 3. 2019 09:43.
Anotace
This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh1, AdoR1 (adenosine receptor), and Akh1 AdoR1. Mortality decreased in all mutants post-EPN infection compared with the control (w1118). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w1118 and Akh1 larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR1 and Akh1 AdoR1 mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh1. In uninfected larvae mutations decreased antioxidative capacity in Akh1 and increased in AdoR1, however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w1118 larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.
Anotace česky
This study examined how adipokinetic hormone (AKH) and adenosine affect defense responses in Drosophila melanogaster larvae infected with entomopathogenic nematodes (EPN, Steinernema carpocapsae and Heterorhabditis bacteriophora). Three loss-of-function mutant larvae were tested: Akh1, AdoR1 (adenosine receptor), and Akh1 AdoR1. Mortality decreased in all mutants post-EPN infection compared with the control (w1118). Additionally, co-application of external AKH with EPN significantly increased mortality beyond rates observed in EPN-only treatment, while also elevating carbon dioxide production, a measure of metabolism. Furthermore trehalose levels increased in both w1118 and Akh1 larvae post-EPN infection, but the latter group exhibited a lower increase and total trehalose levels. Interestingly, baseline trehalose was relatively high in untreated AdoR1 and Akh1 AdoR1 mutants, with levels remaining unaffected by infection. Infection also elevated haemolymph lipid content overall, but the different mutations did not substantially influence this change. In contrast, haemolymph protein content dropped after EPN infection in all tested groups, but this decline was more intense among Akh1. In uninfected larvae mutations decreased antioxidative capacity in Akh1 and increased in AdoR1, however, its post-infection increases were similar in all mutants, suggesting that antioxidant response in Drosophila involves mechanisms also beyond AKH and adenosine. Furthermore, AKH application in w1118 larvae significantly increased movement distance and percentage of larval activity, but reduced velocity. Mutations of Akh and AdoR did not strongly affect locomotion.
Návaznosti
GA17-03253S, projekt VaVNázev: Hormonální kontrola hmyzího obranného systému
Investor: Grantová agentura ČR, Standardní projekty
VytisknoutZobrazeno: 21. 8. 2019 03:40