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@proceedings{1415978,
author = {Machálková, Markéta and Navrátilová, Jarmila and Pavlatovská, Barbora and Šmarda, Jan and Preisler, Jan},
booktitle = {ČSHS 2018},
keywords = {perifosine, spheroid, MALDI, mass spectrometry, confocal microscope},
language = {eng},
title = {The study of perifosine in colorectal carcinoma spheroids by MALDI TOF MSI and confocal microscopy},
year = {2018}
}
TY - CONF
ID - 1415978
AU - Machálková, Markéta - Navrátilová, Jarmila - Pavlatovská, Barbora - Šmarda, Jan - Preisler, Jan
PY - 2018
TI - The study of perifosine in colorectal carcinoma spheroids by MALDI TOF MSI and confocal microscopy
KW - perifosine, spheroid, MALDI, mass spectrometry, confocal microscope
N2 - In our study, the spheroids derived from colorectal carcinoma and potential cancerostatic perifosine were studied. The cellular objects were treated by perifosine for selected time interval (2, 8 and 24 hours) and stained by two viability markers one hour before processing for matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI), according to previously published protocol. The analysis of sections under confocal microscope was carried out before matrix application (in our case DHB sublimation). The examination of selected fluorescent molecules provided information about cell viability across the section, which was then related to perifosine distribution detected by MALDI MSI. Interestingly, both perifosine and the live cell marker were found to diffuse from spheroid to gelatine during sample preparation.
ER -
MACHÁLKOVÁ, Markéta, Jarmila NAVRÁTILOVÁ, Barbora PAVLATOVSKÁ, Jan ŠMARDA a Jan PREISLER. The study of perifosine in colorectal carcinoma spheroids by MALDI TOF MSI and confocal microscopy. In \textit{ČSHS 2018}. 2018.
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