Association of NOD-like receptor (NLRP3) gene variability with
chronic periodontitis and type 2 diabetes mellitus

a 2018

Association of NOD-like receptor (NLRP3) gene variability with chronic periodontitis and type 2 diabetes mellitus

BOŘILOVÁ LINHARTOVÁ, Petra; Lucie MASOPUSTOVÁ; Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ

Základní údaje

Originální název

Association of NOD-like receptor (NLRP3) gene variability with chronic periodontitis and type 2 diabetes mellitus

Autoři

BOŘILOVÁ LINHARTOVÁ, Petra; Lucie MASOPUSTOVÁ; Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ

Vydání

EuroPerio9, Amsterdam, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30101 Human genetics

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/18:00101050

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

chronic periodontitis; diabetes mellitus; gene polymorphism; nod-like receptor 3; case-control study

Změněno: 15. 1. 2019 12:09, doc. RNDr. Petra Bořilová Linhartová, Ph.D., MBA

Anotace

V originále

Background and Aim: Chronic periodontitis (CP) and type 2 diabetes mellitus (T2DM) are diseases characterized by an inflammation; the relationship between them is bidirectional. The NOD like receptor (NLRP3) is a part of inflammasome which is responsible for the maturation of proinflammatory cytokines and it also regulates Th2 differentiation. The aim of our study was to investigate three NLRP3 single nucleotide polymorphisms (SNPs) in diabetic patients with CP and systematically healthy subjects with/without CP in the Czech population. Methods: In total, 486 individuals were included in this case-control study: 85 patients with T2DM+CP, 210 patients with CP and 191 non-periodontitis controls. The subjects were examined by a periodontist and several parameters such as plaque index, gingival index, clinical attachment loss and the presence of selected bacteria were recorded. NLRP3 SNPs (rs4612666, rs10754558, rs3806265) were determined by the method based on qPCR using 5 nuclease TaqMan® assays. Results: Although distribution of allele and genotype frequencies of two NLRP3 SNPs (rs10754558, rs3806265) between patients and controls were similar, carriers of TT genotype vs. carriers of CT+CC genotypes NLRP3 (rs4612666) had a higher risk for development of CP in systemically healthy patients and in T2DM patients (P<0.01 and P<0.05, respectively). This finding was confirmed by haplotype analysis, TGC haplotype NLRP3 (rs4612666/rs10754558/rs3806265) was found as a risk factor for CP (OR=2.46, 95%CI=1.17-5.16, P=0.01). Conclusions: It is possible that lower NLRP3 expression in TT rs4612666 carriers has an impact on CD4+ Th1/Th2 balance. We suggest that variability in the NLRP3 gene may play an important role in the pathogenic pathways of CP in the Czech population. Acknowledgements: The study was supported by grant GACR GB14-37368G, funds provided by the Faculty of Medicine MU to junior researcher Petra Borilova Linhartova, and project MUNI/A/0948/2016.

Návaznosti

GB14-37368G, projekt VaV

Název: Centrum orofaciálního vývoje a regenerace

Investor: Grantová agentura ČR, Centrum orofaciálního vývoje a regenerace

MUNI/A/0948/2016, interní kód MU

Název: Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba

Investor: Masarykova univerzita, Nemoci dutiny ústní – etiopatogeneze, klinické projevy, diagnostika a léčba, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

ROZV/25/LF/2017, interní kód MU

Název: LF - Příspěvek na IP 2017

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek na IP 2017, Interní rozvojové projekty

Citovat

BOŘILOVÁ LINHARTOVÁ, Petra; Lucie MASOPUSTOVÁ; Hana POSKEROVÁ a Lydie IZAKOVIČOVÁ HOLLÁ. Association of NOD-like receptor (NLRP3) gene variability with chronic periodontitis and type 2 diabetes mellitus. In EuroPerio9, Amsterdam. 2018.

@proceedings{1423235,
   author = {Bořilová Linhartová, Petra and Masopustová, Lucie and Poskerová, Hana and Izakovičová Hollá, Lydie},
   booktitle = {EuroPerio9, Amsterdam},
   keywords = {chronic periodontitis; diabetes mellitus; gene polymorphism; nod-like receptor 3; case-control study},
   language = {eng},
   title = {Association of NOD-like receptor (NLRP3) gene variability with chronic periodontitis and type 2 diabetes mellitus},
   year = {2018}
}

TY  - CONF
ID  - 1423235
AU  - Bořilová Linhartová, Petra - Masopustová, Lucie - Poskerová, Hana - Izakovičová Hollá, Lydie
PY  - 2018
TI  - Association of NOD-like receptor (NLRP3) gene variability with chronic periodontitis and type 2 diabetes mellitus
KW  - chronic periodontitis
KW  - diabetes mellitus
KW  - gene polymorphism
KW  - nod-like receptor 3
KW  - case-control study
N2  - Background and Aim: Chronic periodontitis (CP) and type 2 diabetes mellitus (T2DM) are diseases characterized by an inflammation; the relationship between them is bidirectional. The NOD like receptor (NLRP3) is a part of inflammasome which is responsible for the maturation of proinflammatory cytokines and it also regulates Th2 differentiation. The aim of our study was to investigate three NLRP3 single nucleotide polymorphisms (SNPs) in diabetic patients with CP and systematically healthy subjects with/without CP in the Czech population. Methods: In total, 486 individuals were included in this case-control study: 85 patients with T2DM+CP, 210 patients with CP and 191 non-periodontitis controls. The subjects were examined by a periodontist and several parameters such as plaque index, gingival index, clinical attachment loss and the presence of selected bacteria were recorded. NLRP3 SNPs (rs4612666, rs10754558, rs3806265) were determined by the method based on qPCR using 5 nuclease TaqMan® assays. Results: Although distribution of allele and genotype frequencies of two NLRP3 SNPs (rs10754558, rs3806265) between patients and controls were similar, carriers of TT genotype vs. carriers of CT+CC genotypes NLRP3 (rs4612666) had a higher risk for development of CP in systemically healthy patients and in T2DM patients (P<0.01 and P<0.05, respectively). This finding was confirmed by haplotype analysis, TGC haplotype NLRP3 (rs4612666/rs10754558/rs3806265) was found as a risk factor for CP (OR=2.46, 95%CI=1.17-5.16, P=0.01). Conclusions: It is possible that lower NLRP3 expression in TT rs4612666 carriers has an impact on CD4+ Th1/Th2 balance. We suggest that variability in the NLRP3 gene may play an important role in the pathogenic pathways of CP in the Czech population. Acknowledgements: The study was supported by grant GACR GB14-37368G, funds provided by the Faculty of Medicine MU to junior researcher Petra Borilova Linhartova, and project MUNI/A/0948/2016.
ER  -

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