Failure to diverge connected with low rate of molecular
evolution in an African freshwater parasitic flatworm
(Monogenea, Diplectanidae)

k 2018

Failure to diverge connected with low rate of molecular evolution in an African freshwater parasitic flatworm (Monogenea, Diplectanidae)

KMENTOVÁ, Nikol; M. VAN STEENBERGE; S. KOBLMÜLLER; F. MUTEREZI BUKINGA; T. MULIMBWA N SIBULA et al.

Základní údaje

Originální název

Failure to diverge connected with low rate of molecular evolution in an African freshwater parasitic flatworm (Monogenea, Diplectanidae)

Autoři

KMENTOVÁ, Nikol; M. VAN STEENBERGE; S. KOBLMÜLLER; F. MUTEREZI BUKINGA; T. MULIMBWA N SIBULA; P. MASILYA MULUNGULA; Milan GELNAR a Maarten Pieterjan VANHOVE

Vydání

Helminthological Days, 2018

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10613 Zoology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/18:00101203

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Latidae; morphometrics; population structure

Změněno: 25. 9. 2018 23:52, Mgr. Nikol Kmentová, Ph.D.

Anotace

V originále

Universal species identification using specific molecular tags derived from the cytochrome c oxidase subunit 1 gene (COI) of the mitochondrial DNA, has successfully been implemented in many taxonomic groups. Correspondingly, molecular characterisation has revealed cryptic species. However, incongruence between morphological and genetic results in well-studied monogenean species was documented in members of Diplectanidae (Monogenea). In our study, we tested the link between the level of genetic and morphological differentiation for diplectanid parasites infecting latid hosts in geographically isolated areas in Lake Albert and Lake Tanganyika. Specimens from five African latid species (Lates angustifrons, L. mariae, L. microlepis, L. niloticus, L. stappersii) were examined for the presence of monogenean gill parasites from several localities. Monogeneans were characterised morphologically via morphometrics of sclerotised structures and genetically using markers with different rates of molecular evolution from nuclear ribosomal and mitochondrial DNA regions. In total, four out of five latid species were infected by monogenean parasites belonging to Diplectanum. Continuous morphological variation was documented, and the genetic distance between parasites of geographically isolated host species in the COI region did not reach the previously published threshold of 14.5%. Therefore, a single species, D. lacustre, is suggested to infect latids in both lakes. Based on the age of the eastern African Rift Lake system (9 MYA), the substitution rate in the COI region of D. lacustre was estimated to be 0.5%. Despite the persistent geographic barrier, D. lacustre has not reached the threshold of genetic differentiation in COI gene proposed for species delineation of diplectanid monogeneans. Congruence between the slow rate of molecular evolution in the mitochondrial COI region and the level of parasite morphological differentiation is suggested. Universal species identification using specific molecular tags derived from the cytochrome c oxidase subunit 1 gene (COI) of the mitochondrial DNA, has successfully been implemented in many taxonomic groups. Correspondingly, molecular characterisation has revealed cryptic species. However, incongruence between morphological and genetic results in well-studied monogenean species was documented in members of Diplectanidae (Monogenea). In our study, we tested the link between the level of genetic and morphological differentiation for diplectanid parasites infecting latid hosts in geographically isolated areas in Lake Albert and Lake Tanganyika. Specimens from five African latid species (Lates angustifrons, L. mariae, L. microlepis, L. niloticus, L. stappersii) were examined for the presence of monogenean gill parasites from several localities. Monogeneans were characterised morphologically via morphometrics of sclerotised structures and genetically using markers with different rates of molecular evolution from nuclear ribosomal and mitochondrial DNA regions. In total, four out of five latid species were infected by monogenean parasites belonging to Diplectanum. Continuous morphological variation was documented, and the genetic distance between parasites of geographically isolated host species in the COI region did not reach the previously published threshold of 14.5%. Therefore, a single species, D. lacustre, is suggested to infect latids in both lakes. Based on the age of the eastern African Rift Lake system (9 MYA), the substitution rate in the COI region of D. lacustre was estimated to be 0.5%. Despite the persistent geographic barrier, D. lacustre has not reached the threshold of genetic differentiation in COI gene proposed for species delineation of diplectanid monogeneans. Congruence between the slow rate of molecular evolution in the mitochondrial COI region and the level of parasite morphological differentiation is suggested.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

KMENTOVÁ, Nikol; M. VAN STEENBERGE; S. KOBLMÜLLER; F. MUTEREZI BUKINGA; T. MULIMBWA N SIBULA; P. MASILYA MULUNGULA; Milan GELNAR a Maarten Pieterjan VANHOVE. Failure to diverge connected with low rate of molecular evolution in an African freshwater parasitic flatworm (Monogenea, Diplectanidae). In Helminthological Days. 2018.

@proceedings{1441582,
   author = {Kmentová, Nikol and Van Steenberge, M. and Koblmüller, S. and Muterezi Bukinga, F. and Mulimbwa N sibula, T. and Masilya Mulungula, P. and Gelnar, Milan and Vanhove, Maarten Pieterjan},
   booktitle = {Helminthological Days},
   keywords = {Latidae; morphometrics; population structure},
   language = {eng},
   title = {Failure to diverge connected with low rate of molecular evolution in an African freshwater parasitic flatworm (Monogenea, Diplectanidae)},
   year = {2018}
}

TY  - CONF
ID  - 1441582
AU  - Kmentová, Nikol - Van Steenberge, M. - Koblmüller, S. - Muterezi Bukinga, F. - Mulimbwa N sibula, T. - Masilya Mulungula, P. - Gelnar, Milan - Vanhove, Maarten Pieterjan
PY  - 2018
TI  - Failure to diverge connected with low rate of molecular evolution in an African freshwater parasitic flatworm (Monogenea, Diplectanidae)
KW  - Latidae
KW  - morphometrics
KW  - population structure
N2  - Universal species identification using specific molecular tags derived from the cytochrome c oxidase subunit 1 gene (COI) of the mitochondrial DNA, has successfully been implemented in many taxonomic groups. Correspondingly, molecular characterisation has revealed cryptic species. However, incongruence between morphological and genetic results in well-studied monogenean species was documented in members of Diplectanidae (Monogenea). In our study, we tested the link between the level of genetic and morphological differentiation for diplectanid parasites infecting latid hosts in geographically isolated areas in Lake Albert and Lake Tanganyika. Specimens from five African latid species (Lates angustifrons, L. mariae, L. microlepis, L. niloticus, L. stappersii) were examined for the presence of monogenean gill parasites from several localities. Monogeneans were characterised morphologically via morphometrics of sclerotised structures and genetically using markers with different rates of molecular evolution from nuclear ribosomal and mitochondrial DNA regions. In total, four out of five latid species were infected by monogenean parasites belonging to Diplectanum. Continuous morphological variation was documented, and the genetic distance between parasites of geographically isolated host species in the COI region did not reach the previously published threshold of 14.5%. Therefore, a single species, D. lacustre, is suggested to infect latids in both lakes. Based on the age of the eastern African Rift Lake system (9 MYA), the substitution rate in the COI region of D. lacustre was estimated to be 0.5%. Despite the persistent geographic barrier, D. lacustre has not reached the threshold of genetic differentiation in COI gene proposed for species delineation of diplectanid monogeneans. Congruence between the slow rate of molecular evolution in the mitochondrial COI region and the level of parasite morphological differentiation is suggested. Universal species identification using specific molecular tags derived from the cytochrome c oxidase subunit 1 gene (COI) of the mitochondrial DNA, has successfully been implemented in many taxonomic groups. Correspondingly, molecular characterisation has revealed cryptic species. However, incongruence between morphological and genetic results in well-studied monogenean species was documented in members of Diplectanidae (Monogenea). In our study, we tested the link between the level of genetic and morphological differentiation for diplectanid parasites infecting latid hosts in geographically isolated areas in Lake Albert and Lake Tanganyika. Specimens from five African latid species (Lates angustifrons, L. mariae, L. microlepis, L. niloticus, L. stappersii) were examined for the presence of monogenean gill parasites from several localities. Monogeneans were characterised morphologically via morphometrics of sclerotised structures and genetically using markers with different rates of molecular evolution from nuclear ribosomal and mitochondrial DNA regions. In total, four out of five latid species were infected by monogenean parasites belonging to Diplectanum. Continuous morphological variation was documented, and the genetic distance between parasites of geographically isolated host species in the COI region did not reach the previously published threshold of 14.5%. Therefore, a single species, D. lacustre, is suggested to infect latids in both lakes. Based on the age of the eastern African Rift Lake system (9 MYA), the substitution rate in the COI region of D. lacustre was estimated to be 0.5%. Despite the persistent geographic barrier, D. lacustre has not reached the threshold of genetic differentiation in COI gene proposed for species delineation of diplectanid monogeneans. Congruence between the slow rate of molecular evolution in the mitochondrial COI region and the level of parasite morphological differentiation is suggested.
ER  -

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