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@article{1504904,
author = {Špírek, Mário and Mlčoušková, Jarmila and Beláň, Ondrej and Gyimesi, Máté and Harami, Gábor M. and Molnár, Eszter and Nováček, Jiří and Kovács, Mihály and Krejčí, Lumír},
article_location = {Oxford},
article_number = {8},
doi = {http://dx.doi.org/10.1093/nar/gky111},
keywords = {RAD51},
language = {eng},
issn = {0305-1048},
journal = {Nucleic Acids Research},
title = {Human RAD51 rapidly forms intrinsically dynamic nucleoprotein filaments modulated by nucleotide binding state},
volume = {46},
year = {2018}
}
TY - JOUR
ID - 1504904
AU - Špírek, Mário - Mlčoušková, Jarmila - Beláň, Ondrej - Gyimesi, Máté - Harami, Gábor M. - Molnár, Eszter - Nováček, Jiří - Kovács, Mihály - Krejčí, Lumír
PY - 2018
TI - Human RAD51 rapidly forms intrinsically dynamic nucleoprotein filaments modulated by nucleotide binding state
JF - Nucleic Acids Research
VL - 46
IS - 8
SP - 3967-3980
EP - 3967-3980
PB - Oxford University Press
SN - 03051048
KW - RAD51
N2 - Formation of RAD51 filaments on single-stranded DNA is an essential event during homologous recombination, which is required for homology search, strand exchange and protection of replication forks. Formation of nucleoprotein filaments (NF) is required for development and genomic stability, and its failure is associated with developmental abnormalities and tumorigenesis. Here we describe the structure of the human RAD51 NFs and of its Walker box mutants using electron microscopy. Wild-type RAD51 filaments adopt an 'open' conformation when compared to a 'closed' structure formed by mutants, reflecting alterations in helical pitch. The kinetics of formation/disassembly of RAD51 filaments show rapid and high ssDNA coverage via low cooperativity binding of RAD51 units along the DNA. Subsequently, a series of isomerization or dissociation events mediated by nucleotide binding state creates intrinsically dynamic RAD51 NFs. Our findings highlight important a mechanistic divergence among recombinases from different organisms, in line with the diversity of biological mechanisms of HR initiation and quality control. These data reveal unexpected intrinsic dynamic properties of the RAD51 filament during assembly/disassembly, which may be important for the proper control of homologous recombination.
ER -
ŠPÍREK, Mário, Jarmila MLČOUŠKOVÁ, Ondrej BELÁŇ, Máté GYIMESI, Gábor M. HARAMI, Eszter MOLNÁR, Jiří NOVÁČEK, Mihály KOVÁCS a Lumír KREJČÍ. Human RAD51 rapidly forms intrinsically dynamic nucleoprotein filaments modulated by nucleotide binding state. \textit{Nucleic Acids Research}. Oxford: Oxford University Press, roč.~46, č.~8, s.~3967-3980. ISSN~0305-1048. doi:10.1093/nar/gky111. 2018.
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