MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per
Second

J 2019

MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

BEDNAŘÍK, Antonín; Markéta MACHÁLKOVÁ; Eugene MOSKOVETS; Kateřina COUFALÍKOVÁ; Pavel KRÁSENSKÝ et. al.

Základní údaje

Originální název

MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second

Autoři

BEDNAŘÍK, Antonín (203 Česká republika, domácí); Markéta MACHÁLKOVÁ (203 Česká republika, domácí); Eugene MOSKOVETS (840 Spojené státy); Kateřina COUFALÍKOVÁ (203 Česká republika, domácí); Pavel KRÁSENSKÝ (203 Česká republika, domácí); Pavel HOUŠKA (203 Česká republika); Jiří KROUPA (203 Česká republika); Jarmila NAVRÁTILOVÁ (203 Česká republika, domácí); Jan ŠMARDA (203 Česká republika, domácí) a Jan PREISLER (203 Česká republika, garant, domácí)

Vydání

Journal of the American Society for Mass Spectrometry, New York, Springer, 2019, 1044-0305

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Full Text

Impakt faktor

Impact factor: 3.255

Kód RIV

RIV/00216224:14310/19:00107314

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1007/s13361-018-2078-8

UT WoS

000457210600011

EID Scopus

2-s2.0-85060540749

Klíčová slova anglicky

Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 3. 2020 16:00, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s.

Návaznosti

GA15-05387S, projekt VaV

Název: Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

Investor: Grantová agentura ČR, Laserově desorpční hmotnostní spektrometrie pro analýzu molekul a nanočástic

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

MUNI/G/0974/2016, interní kód MU

Název: Optimalizace cílené terapie kolorektálního karcinomu ovlivňováním účinnosti penetrace a cytotoxicity regulátorů buněčných signalizací

Investor: Masarykova univerzita, Optimalizace cílené terapie kolorektálního karcinomu ovlivňováním účinnosti penetrace a cytotoxicity regulátorů buněčných signalizací, INTERDISCIPLINARY - Mezioborové výzkumné projekty

Citovat

BEDNAŘÍK, Antonín; Markéta MACHÁLKOVÁ; Eugene MOSKOVETS; Kateřina COUFALÍKOVÁ; Pavel KRÁSENSKÝ; Pavel HOUŠKA; Jiří KROUPA; Jarmila NAVRÁTILOVÁ; Jan ŠMARDA a Jan PREISLER. MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second. Journal of the American Society for Mass Spectrometry. New York: Springer, 2019, roč. 30, č. 2, s. 289-298. ISSN 1044-0305. Dostupné z: https://dx.doi.org/10.1007/s13361-018-2078-8.

@article{1504977,
   author = {Bednařík, Antonín and Machálková, Markéta and Moskovets, Eugene and Coufalíková, Kateřina and Krásenský, Pavel and Houška, Pavel and Kroupa, Jiří and Navrátilová, Jarmila and Šmarda, Jan and Preisler, Jan},
   article_location = {New York},
   article_number = {2},
   doi = {http://dx.doi.org/10.1007/s13361-018-2078-8},
   keywords = {Mass spectrometry imaging; Laser beam scanning; MALDI; TOF; MSI; High throughput; Grid ion source; 3D cell aggregates; Spheroids; Colorectal adenocarcinoma},
   language = {eng},
   issn = {1044-0305},
   journal = {Journal of the American Society for Mass Spectrometry},
   title = {MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second},
   url = {https://link.springer.com/article/10.1007/s13361-018-2078-8},
   volume = {30},
   year = {2019}
}

TY  - JOUR
ID  - 1504977
AU  - Bednařík, Antonín - Machálková, Markéta - Moskovets, Eugene - Coufalíková, Kateřina - Krásenský, Pavel - Houška, Pavel - Kroupa, Jiří - Navrátilová, Jarmila - Šmarda, Jan - Preisler, Jan
PY  - 2019
TI  - MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second
JF  - Journal of the American Society for Mass Spectrometry
VL  - 30
IS  - 2
SP  - 289-298
EP  - 289-298
PB  - Springer
SN  - 10440305
KW  - Mass spectrometry imaging
KW  - Laser beam scanning
KW  - MALDI
KW  - TOF
KW  - MSI
KW  - High throughput
KW  - Grid ion source
KW  - 3D cell aggregates
KW  - Spheroids
KW  - Colorectal adenocarcinoma
UR  - https://link.springer.com/article/10.1007/s13361-018-2078-8
L2  - https://link.springer.com/article/10.1007/s13361-018-2078-8
N2  - The practicality of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) applied to molecular imaging of biological tissues is limited by the analysis speed. Typically, a relatively low speed of stop-and-go micromotion of XY stages is considered as a factor substantially reducing the rate with which fresh sample material can be supplied to the laser spot. The sample scan rate in our laboratory-built high-throughput imaging TOF mass spectrometer was significantly improved through the use of a galvanometer-based optical scanner performing fast laser spot repositioning on a target plate. The optical system incorporated into the ion source of our MALDI TOF mass spectrometer allowed focusing the laser beam via a modified grid into a 10-m round spot. This permitted the acquisition of high-resolution MS images with a well-defined pixel size at acquisition rates exceeding 100pixel/s. The influence of selected parameters on the total MS imaging time is discussed. The new scanning technique was employed to display the distribution of an antitumor agent in 3D colorectal adenocarcinoma cell aggregates; a single MS image comprising 100x100pixels with 10-m lateral resolution was recorded in approximately 70s.
ER  -

BEDNAŘÍK, Antonín; Markéta MACHÁLKOVÁ; Eugene MOSKOVETS; Kateřina COUFALÍKOVÁ; Pavel KRÁSENSKÝ; Pavel HOUŠKA; Jiří KROUPA; Jarmila NAVRÁTILOVÁ; Jan ŠMARDA a Jan PREISLER. MALDI MS Imaging at Acquisition Rates Exceeding 100 Pixels per Second. \textit{Journal of the American Society for Mass Spectrometry}. New York: Springer, 2019, roč.~30, č.~2, s.~289-298. ISSN~1044-0305. Dostupné z: https://dx.doi.org/10.1007/s13361-018-2078-8.

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