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@article{1546294, author = {Vysloužil, Jakub and Vetchý, David and Zeman, Jiří and Farsa, Oldřich and Franc, Aleš and Gajdziok, Jan and Vysloužil, Jan and Ficeriova, Katarina and Kulich, Pavel and Kobliha, Zbynek and Pitschmann, Vladimir}, article_location = {Amsterdam}, article_number = {August}, doi = {http://dx.doi.org/10.1016/j.jpba.2018.08.050}, keywords = {Pellets; Coating; Immobilization; Butyrylcholinesterase; Soman; Detection}, language = {eng}, issn = {0731-7085}, journal = {Journal of Pharmaceutical and Biomedical Analysis}, title = {Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids}, url = {https://www.sciencedirect.com/science/article/pii/S073170851830880X?via%3Dihub}, volume = {161}, year = {2018} }
TY - JOUR ID - 1546294 AU - Vysloužil, Jakub - Vetchý, David - Zeman, Jiří - Farsa, Oldřich - Franc, Aleš - Gajdziok, Jan - Vysloužil, Jan - Ficeriova, Katarina - Kulich, Pavel - Kobliha, Zbynek - Pitschmann, Vladimir PY - 2018 TI - Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids JF - Journal of Pharmaceutical and Biomedical Analysis VL - 161 IS - August SP - 206-213 EP - 206-213 PB - Elsevier Science BV SN - 07317085 KW - Pellets KW - Coating KW - Immobilization KW - Butyrylcholinesterase KW - Soman KW - Detection UR - https://www.sciencedirect.com/science/article/pii/S073170851830880X?via%3Dihub L2 - https://www.sciencedirect.com/science/article/pii/S073170851830880X?via%3Dihub N2 - The main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbamate insecticides in liquids that is observable visually and also spectrophotometrically at 412 nm. The pellet cores were prepared by the extrusion/spheronization method. Consecutively, two different coats were applied on the pellet cores in the coating device using the Wurster column method. To increase the color change intensity, the second semipermeable coat based on Eudragit (R) RL was applied on top of the first coat, which was formed by butyrylcholinesterase immobilized in hydroxypropyl methylcellulose. Prepared samples differing in thickness of the second coat were evaluated for their quality parameters, enzymatic activity and inhibition. The detection mechanism was based on the standard Ellman's colorimetric reaction. It was observed that the semipermeable coat prevented leaching of the enzyme into the solution and led to an increased intensity of color transition from white - yellow to white - deep yellow/orange, thus enabling a more accurate visual detection. This system allows easy, rapid and safe identification of cholinesterase inhibitors in liquids, especially chemical warfare agents. ER -
VYSLOUŽIL, Jakub, David VETCHÝ, Jiří ZEMAN, Oldřich FARSA, Aleš FRANC, Jan GAJDZIOK, Jan VYSLOUŽIL, Katarina FICERIOVA, Pavel KULICH, Zbynek KOBLIHA a Vladimir PITSCHMANN. Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids. \textit{Journal of Pharmaceutical and Biomedical Analysis}. Amsterdam: Elsevier Science BV, 2018, roč.~161, August, s.~206-213. ISSN~0731-7085. Dostupné z: https://dx.doi.org/10.1016/j.jpba.2018.08.050.
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