VYSLOUŽIL, Jakub, David VETCHÝ, Jiří ZEMAN, Oldřich FARSA, Aleš FRANC, Jan GAJDZIOK, Jan VYSLOUŽIL, Katarina FICERIOVA, Pavel KULICH, Zbynek KOBLIHA a Vladimir PITSCHMANN. Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids. Journal of Pharmaceutical and Biomedical Analysis. Amsterdam: Elsevier Science BV, 2018, roč. 161, August, s. 206-213. ISSN 0731-7085. doi:10.1016/j.jpba.2018.08.050.
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Základní údaje
Originální název Pellet patented technology for fast and distinct visual detection of cholinesterase inhibitors in liquids
Autoři VYSLOUŽIL, Jakub (203 Česká republika), David VETCHÝ (203 Česká republika, garant), Jiří ZEMAN (203 Česká republika), Oldřich FARSA (203 Česká republika), Aleš FRANC (203 Česká republika), Jan GAJDZIOK (203 Česká republika), Jan VYSLOUŽIL (203 Česká republika, domácí), Katarina FICERIOVA (203 Česká republika), Pavel KULICH (203 Česká republika), Zbynek KOBLIHA (203 Česká republika) a Vladimir PITSCHMANN (203 Česká republika).
Vydání Journal of Pharmaceutical and Biomedical Analysis, Amsterdam, Elsevier Science BV, 2018, 0731-7085.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 30104 Pharmacology and pharmacy
Stát vydavatele Nizozemsko
Utajení není předmětem státního či obchodního tajemství
WWW Full Text
Impakt faktor Impact factor: 2.983
Kód RIV RIV/00216224:14310/18:00110215
Organizační jednotka Přírodovědecká fakulta
Doi http://dx.doi.org/10.1016/j.jpba.2018.08.050
UT WoS 000447682300023
Klíčová slova anglicky Pellets; Coating; Immobilization; Butyrylcholinesterase; Soman; Detection
Štítky rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Marie Šípková, DiS., učo 437722. Změněno: 23. 6. 2020 09:27.
Anotace
The main objective of the presented research was to prepare an innovative carrier as a filler for detection tubes in the form of double-coated pellets with a very significant color transition during the detection of cholinesterase inhibitors such as nerve agents, organophosphorus or carbamate insecticides in liquids that is observable visually and also spectrophotometrically at 412 nm. The pellet cores were prepared by the extrusion/spheronization method. Consecutively, two different coats were applied on the pellet cores in the coating device using the Wurster column method. To increase the color change intensity, the second semipermeable coat based on Eudragit (R) RL was applied on top of the first coat, which was formed by butyrylcholinesterase immobilized in hydroxypropyl methylcellulose. Prepared samples differing in thickness of the second coat were evaluated for their quality parameters, enzymatic activity and inhibition. The detection mechanism was based on the standard Ellman's colorimetric reaction. It was observed that the semipermeable coat prevented leaching of the enzyme into the solution and led to an increased intensity of color transition from white - yellow to white - deep yellow/orange, thus enabling a more accurate visual detection. This system allows easy, rapid and safe identification of cholinesterase inhibitors in liquids, especially chemical warfare agents.
VytisknoutZobrazeno: 2. 2. 2023 16:58