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@article{1624157,
author = {Pospíšilová, Veronika and Ešner, Milan and Červenková, Iveta and Fedr, Radek and Tinevez, JeanandYvez and Hampl, Aleš and Anger, Martin},
article_location = {České Budějovice},
article_number = {4},
doi = {http://dx.doi.org/10.32725/jab.2019.018},
keywords = {Embryonic stem (ES) cells; Mitosis length; Multipolar division; Single-cell tracking; Spindle assembly checkpoint (SAC); Time-lapse microscopy},
language = {eng},
issn = {1214-021X},
journal = {Journal of applied biomedicine},
title = {The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells},
url = {http://dx.doi.org/10.32725/jab.2019.018},
volume = {17},
year = {2019}
}
TY - JOUR
ID - 1624157
AU - Pospíšilová, Veronika - Ešner, Milan - Červenková, Iveta - Fedr, Radek - Tinevez, Jean-Yvez - Hampl, Aleš - Anger, Martin
PY - 2019
TI - The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells
JF - Journal of applied biomedicine
VL - 17
IS - 4
SP - 209-217
EP - 209-217
PB - Faculty of Health and Social Care, University of South Bohemia
SN - 1214021X
KW - Embryonic stem (ES) cells
KW - Mitosis length
KW - Multipolar division
KW - Single-cell tracking
KW - Spindle assembly checkpoint (SAC)
KW - Time-lapse microscopy
UR - http://dx.doi.org/10.32725/jab.2019.018
L2 - http://dx.doi.org/10.32725/jab.2019.018
N2 - Embryonic stem (ES) cells are pluripotent cells widely used in cell therapy and tissue engineering. However, the broader clinical applications of ES cells are limited by their genomic instability and karyotypic abnormalities. Thus, understanding the mechanisms underlying ES cell karyotypic abnormalities is critical to optimizing their dinical use. In this study, we focused on proliferating human and mouse ES cells undergoing multipolar divisions. Specifically, we analyzed the frequency and outcomes of such divisions using a combination of time-lapse microscopy and cell tracking. This revealed that cells resulting from multipolar divisions were not only viable, but they also frequently underwent subsequent cell divisions. Our novel data also showed that in human and mouse ES cells, multipolar spindles allowed more robust escape from chromosome segregation control mechanisms than bipolar spindles. Considering the frequency of multipolar divisions in proliferating ES cells, it is conceivable that cell division errors underlie ES cell karyotypic instability.
ER -
POSPÍŠILOVÁ, Veronika, Milan EŠNER, Iveta ČERVENKOVÁ, Radek FEDR, Jean-Yvez TINEVEZ, Aleš HAMPL a Martin ANGER. The frequency and consequences of multipolar mitoses in undifferentiated embryonic stem cells. \textit{Journal of applied biomedicine}. České Budějovice: Faculty of Health and Social Care, University of South Bohemia, roč.~17, č.~4, s.~209-217. ISSN~1214-021X. doi:10.32725/jab.2019.018. 2019.
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