CdS quantum dots-based immunoassay combined with particle
imprinted polymer technology and laser ablation ICP-MS as a
versatile tool for protein detection

J 2019

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

VANECKOVA, T.; J. BEZDEKOVA; Michaela TVRDOŇOVÁ; M. VLCNOVSKA; V. NOVOTNA et. al.

Základní údaje

Originální název

CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection

Autoři

VANECKOVA, T.; J. BEZDEKOVA; Michaela TVRDOŇOVÁ; M. VLCNOVSKA; V. NOVOTNA; J. NEUMAN; Aneta ŠTOSSOVÁ; Viktor KANICKÝ; V. ADAM; M. VACULOVICOVA a Tomáš VACULOVIČ

Vydání

Scientific reports, LONDON, NATURE PUBLISHING GROUP, 2019, 2045-2322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.998

Kód RIV

RIV/00216224:14310/19:00113292

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.1038/s41598-019-48290-2

UT WoS

000480678100039

EID Scopus

2-s2.0-85070782595

Klíčová slova anglicky

MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 3. 2020 19:50, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

VANECKOVA, T.; J. BEZDEKOVA; Michaela TVRDOŇOVÁ; M. VLCNOVSKA; V. NOVOTNA; J. NEUMAN; Aneta ŠTOSSOVÁ; Viktor KANICKÝ; V. ADAM; M. VACULOVICOVA a Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. Scientific reports. LONDON: NATURE PUBLISHING GROUP, 2019, roč. 9, AUG, s. 1-9. ISSN 2045-2322. Dostupné z: https://doi.org/10.1038/s41598-019-48290-2.

@article{1636928,
   author = {Vaneckova, T. and Bezdekova, J. and Tvrdoňová, Michaela and Vlcnovska, M. and Novotna, V. and Neuman, J. and Štossová, Aneta and Kanický, Viktor and Adam, V. and Vaculovicova, M. and Vaculovič, Tomáš},
   article_location = {LONDON},
   article_number = {AUG},
   doi = {https://doi.org/10.1038/s41598-019-48290-2},
   keywords = {MAGNETIC NANOPARTICLES; SENSITIVE DETECTION; SURFACE-CHEMISTRY; RECOGNITION; SENSOR; EXTRACTION; SEPARATION; DNA},
   language = {eng},
   issn = {2045-2322},
   journal = {Scientific reports},
   title = {CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection},
   url = {https://www.nature.com/articles/s41598-019-48290-2#Ack1},
   volume = {9},
   year = {2019}
}

TY  - JOUR
ID  - 1636928
AU  - Vaneckova, T. - Bezdekova, J. - Tvrdoňová, Michaela - Vlcnovska, M. - Novotna, V. - Neuman, J. - Štossová, Aneta - Kanický, Viktor - Adam, V. - Vaculovicova, M. - Vaculovič, Tomáš
PY  - 2019
TI  - CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection
JF  - Scientific reports
VL  - 9
IS  - AUG
SP  - 1-9
EP  - 1-9
PB  - NATURE PUBLISHING GROUP
SN  - 20452322
KW  - MAGNETIC NANOPARTICLES
KW  - SENSITIVE DETECTION
KW  - SURFACE-CHEMISTRY
KW  - RECOGNITION
KW  - SENSOR
KW  - EXTRACTION
KW  - SEPARATION
KW  - DNA
UR  - https://www.nature.com/articles/s41598-019-48290-2#Ack1
L2  - https://www.nature.com/articles/s41598-019-48290-2#Ack1
N2  - For the first time, the combination of molecularly imprinted polymer (MIP) technology with laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) is presented with focus on an optimization of the LA-ICP-MS parameters such as laser beam diameter, laser beam fluence, and scan speed using CdS quantum dots (QDs) as a template and dopamine as a functional monomer. A non-covalent imprinting approach was employed in this study due to the simplicity of preparation. Simple oxidative polymerization of the dopamine that creates the self-assembly monolayer seems to be an ideal choice. The QDs prepared by UV light irradiation synthesis were stabilized by using mercaptosuccinic acid. Formation of a complex of QD-anti body and QD-antibody-antigen was verified by using capillary electrophoresis with laser-induced fluorescence detection. QDs and antibody were connected together via an affinity peptide linker. LA-ICP-MS was employed as a proof-of-concept for detection method of two types of immunoassay: 1) antigen extracted from the sample by MIP and subsequently overlaid/immunoreacted by QD-labelled antibodies, 2) complex of antigen, antibody, and QD formed in the sample and subsequently extracted by MIP. The first approach provided higher sensitivity (MIP/NIP), however, the second demonstrated higher selectivity. A mixture of proteins with size in range 10-250 kDa was used as a model sample to demonstrate the capability of both approaches for detection of IgG in a complex sample.
ER  -

VANECKOVA, T.; J. BEZDEKOVA; Michaela TVRDOŇOVÁ; M. VLCNOVSKA; V. NOVOTNA; J. NEUMAN; Aneta ŠTOSSOVÁ; Viktor KANICKÝ; V. ADAM; M. VACULOVICOVA a Tomáš VACULOVIČ. CdS quantum dots-based immunoassay combined with particle imprinted polymer technology and laser ablation ICP-MS as a versatile tool for protein detection. \textit{Scientific reports}. LONDON: NATURE PUBLISHING GROUP, 2019, roč.~9, AUG, s.~1-9. ISSN~2045-2322. Dostupné z: https://doi.org/10.1038/s41598-019-48290-2.

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