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@article{1637359,
author = {Kasari, V. and Pochopien, A.A. and Margus, T. and Murina, V. and Turnbull, K. and Zhou, Y. and Nissan, T. and Graf, M. and Nováček, Jiří and Atkinson, G.C. and Johansson, M.J.O. and Wilson, D.N. and Hauryliuk, V.},
article_location = {Oxford},
article_number = {16},
doi = {http://dx.doi.org/10.1093/nar/gkz600},
keywords = {MESSENGER-RNA DECAY; INITIATION-FACTOR; GENE ONTOLOGY; YEAST; ELONGATION; COMPLEX; VISUALIZATION; PURIFICATION; BIOGENESIS; EXPRESSION},
language = {eng},
issn = {0305-1048},
journal = {Nucleic acids research},
title = {A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling},
url = {http://sro.sussex.ac.uk/id/eprint/84897/1/gkz600.pdf},
volume = {47},
year = {2019}
}
TY - JOUR
ID - 1637359
AU - Kasari, V. - Pochopien, A.A. - Margus, T. - Murina, V. - Turnbull, K. - Zhou, Y. - Nissan, T. - Graf, M. - Nováček, Jiří - Atkinson, G.C. - Johansson, M.J.O. - Wilson, D.N. - Hauryliuk, V.
PY - 2019
TI - A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling
JF - Nucleic acids research
VL - 47
IS - 16
SP - 8807-8820
EP - 8807-8820
PB - Oxford University Press
SN - 03051048
KW - MESSENGER-RNA DECAY
KW - INITIATION-FACTOR
KW - GENE ONTOLOGY
KW - YEAST
KW - ELONGATION
KW - COMPLEX
KW - VISUALIZATION
KW - PURIFICATION
KW - BIOGENESIS
KW - EXPRESSION
UR - http://sro.sussex.ac.uk/id/eprint/84897/1/gkz600.pdf
L2 - http://sro.sussex.ac.uk/id/eprint/84897/1/gkz600.pdf
N2 - Translation is controlled by numerous accessory proteins and translation factors. In the yeast Saccharomyces cerevisiae, translation elongation requires an essential elongation factor, the ABCF ATPase eEF3. A closely related protein, New1, is encoded by a non-essential gene with cold sensitivity and ribosome assembly defect knock-out phenotypes. Since the exact molecular function of New1 is unknown, it is unclear if the ribosome assembly defect is direct, i.e. New1 is a bona fide assembly factor, or indirect, for instance due to a defect in protein synthesis. To investigate this, we employed yeast genetics, cryo-electron microscopy (cryo-EM) and ribosome profiling (Ribo-Seq) to interrogate the molecular function of New1. Overexpression of New1 rescues the inviability of a yeast strain lacking the otherwise strictly essential translation factor eEF3. The structure of the ATPase-deficient (EQ(2)) New1 mutant locked on the 80S ribosome reveals that New1 binds analogously to the ribosome as eEF3. Finally, Ribo-Seq analysis revealed that loss of New1 leads to ribosome queuing upstream of 3'-terminal lysine and arginine codons, including those genes encoding proteins of the cytoplasmic translational machinery. Our results suggest that New1 is a translation factor that fine-tunes the efficiency of translation termination or ribosome recycling.
ER -
KASARI, V., A.A. POCHOPIEN, T. MARGUS, V. MURINA, K. TURNBULL, Y. ZHOU, T. NISSAN, M. GRAF, Jiří NOVÁČEK, G.C. ATKINSON, M.J.O. JOHANSSON, D.N. WILSON a V. HAURYLIUK. A role for the Saccharomyces cerevisiae ABCF protein New1 in translation termination/recycling. \textit{Nucleic acids research}. Oxford: Oxford University Press, 2019, roč.~47, č.~16, s.~8807-8820. ISSN~0305-1048. Dostupné z: https://dx.doi.org/10.1093/nar/gkz600.
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