VÍŠKOVÁ, Pavlína, Daniel KRAFČÍK, Lukáš TRANTÍREK a Silvie TRANTÍRKOVÁ. In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells. Current Protocols in Nucleic Acid Chemistry. United States: Wiley-Liss Inc, 2019, roč. 76, č. 1, s. 1-14. ISSN 1934-9270. Dostupné z: https://dx.doi.org/10.1002/cpnc.71. |
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@article{1643298, author = {Víšková, Pavlína and Krafčík, Daniel and Trantírek, Lukáš and Trantírková, Silvie}, article_location = {United States}, article_number = {1}, doi = {http://dx.doi.org/10.1002/cpnc.71}, keywords = {DNA; eukaryotic cellin-cell NMR spectroscopy; mammalian cell}, language = {eng}, issn = {1934-9270}, journal = {Current Protocols in Nucleic Acid Chemistry}, title = {In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells}, url = {https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpnc.71}, volume = {76}, year = {2019} }
TY - JOUR ID - 1643298 AU - Víšková, Pavlína - Krafčík, Daniel - Trantírek, Lukáš - Trantírková, Silvie PY - 2019 TI - In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells JF - Current Protocols in Nucleic Acid Chemistry VL - 76 IS - 1 SP - 1-14 EP - 1-14 PB - Wiley-Liss Inc SN - 19349270 KW - DNA KW - eukaryotic cellin-cell NMR spectroscopy KW - mammalian cell UR - https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpnc.71 L2 - https://currentprotocols.onlinelibrary.wiley.com/doi/pdf/10.1002/cpnc.71 N2 - In-cell NMR spectroscopy is a unique tool that enables the study of the structure and dynamics of biomolecules as well as their interactions in the complex environment of living cells at near-to-atomic resolution. In this article, detailed instructions are described for setting up an in-cell NMR experiment for monitoring structures of DNA oligonucleotides introduced into nuclei of living human cells via tailored electroporation. Detailed step-by-step protocols for both the preparation of an in-cell NMR sample as well as protocols for conducting essential control experiments including flow cytometry and confocal microscopy are described. The strengths and limitations of in-cell NMR experiments are discussed. ER -
VÍŠKOVÁ, Pavlína, Daniel KRAFČÍK, Lukáš TRANTÍREK a Silvie TRANTÍRKOVÁ. In-Cell NMR Spectroscopy of Nucleic Acids in Human Cells. \textit{Current Protocols in Nucleic Acid Chemistry}. United States: Wiley-Liss Inc, 2019, roč.~76, č.~1, s.~1-14. ISSN~1934-9270. Dostupné z: https://dx.doi.org/10.1002/cpnc.71.
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