N-Glycan profiling of lung adenocarcinoma in patients at
different stages of disease

J 2020

N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease

LATTOVÁ, Erika; Jana SKŘIČKOVÁ; Jitka HAUSNEROVÁ; Lukáš FROLA; Leoš KŘEN et al.

Základní údaje

Originální název

N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease

Autoři

LATTOVÁ, Erika; Jana SKŘIČKOVÁ; Jitka HAUSNEROVÁ; Lukáš FROLA; Leoš KŘEN; Ivana IHNATOVÁ; Zbyněk ZDRÁHAL; J. BRYANT a M. POPOVIC

Vydání

MODERN PATHOLOGY, NEW YORK, NATURE PUBLISHING GROUP, 2020, 0893-3952

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30109 Pathology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.842

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/20:00115918

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

GLYCOSYLATION; FUCOSYLATION; GLYCOME; PROTEIN; TISSUE

Štítky

14110215, 14110230, CF PROT, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 20:27, Ing. Martina Blahová

Anotace

V originále

Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal(2)GlcNAc(2)Man(3)GlcNAc(2)Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.

Návaznosti

EF17_050/0008496, projekt VaV

Název: MSCAfellow@MUNI

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

90043, velká výzkumná infrastruktura

Název: CIISB

Přiložené soubory

s41379-019-0441-3.pdf Verze souboru

Citovat

LATTOVÁ, Erika; Jana SKŘIČKOVÁ; Jitka HAUSNEROVÁ; Lukáš FROLA; Leoš KŘEN; Ivana IHNATOVÁ; Zbyněk ZDRÁHAL; J. BRYANT a M. POPOVIC. N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease. MODERN PATHOLOGY. NEW YORK: NATURE PUBLISHING GROUP, 2020, roč. 33, č. 6, s. 1146-1156. ISSN 0893-3952. Dostupné z: https://doi.org/10.1038/s41379-019-0441-3.

@article{1669311,
   author = {Lattová, Erika and Skřičková, Jana and Hausnerová, Jitka and Frola, Lukáš and Křen, Leoš and Ihnatová, Ivana and Zdráhal, Zbyněk and Bryant, J. and Popovic, M.},
   article_location = {NEW YORK},
   article_number = {6},
   doi = {https://doi.org/10.1038/s41379-019-0441-3},
   keywords = {GLYCOSYLATION; FUCOSYLATION; GLYCOME; PROTEIN; TISSUE},
   language = {eng},
   issn = {0893-3952},
   journal = {MODERN PATHOLOGY},
   title = {N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease},
   url = {https://www.nature.com/articles/s41379-019-0441-3.pdf},
   volume = {33},
   year = {2020}
}

TY  - JOUR
ID  - 1669311
AU  - Lattová, Erika - Skřičková, Jana - Hausnerová, Jitka - Frola, Lukáš - Křen, Leoš - Ihnatová, Ivana - Zdráhal, Zbyněk - Bryant, J. - Popovic, M.
PY  - 2020
TI  - N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease
JF  - MODERN PATHOLOGY
VL  - 33
IS  - 6
SP  - 1146-1156
EP  - 1146-1156
PB  - NATURE PUBLISHING GROUP
SN  - 08933952
KW  - GLYCOSYLATION
KW  - FUCOSYLATION
KW  - GLYCOME
KW  - PROTEIN
KW  - TISSUE
UR  - https://www.nature.com/articles/s41379-019-0441-3.pdf
L2  - https://www.nature.com/articles/s41379-019-0441-3.pdf
N2  - Lung adenocarcinoma (LAC) is the most common form of lung cancer that increases in non-smokers at younger age. Altered protein glycosylation is one of the hallmarks of malignancy, its role in cancer progression is still poorly understood. In this study, we report mass spectrometric (MS) analysis of N-glycans released from fresh or defrosted tissue specimens from 24 patients with LAC. Comparison of cancerous versus adjacent healthy tissues revealed substantial differences in N-glycan profiles associated with disease. The significant increase in paucimannose and high-mannose glycans with 6-9 mannose residues and decline in the sialylated complex biantenary core fucosylated glycan with composition NeuAcGal(2)GlcNAc(2)Man(3)GlcNAc(2)Fuc were general features of tumors. In addition, 42 new N-glycan compositions were detected in cancerous tissues. The prominent changes in advanced disease stages were mostly observed in core fucosylated N-glycans with additional fucose (Fuc) residue/s and enhanced branching with non-galactosylated N-acetyl-glucosamine (GlcNAc) units. Both of these monosaccharide types were linked preferably on the 6-antenna. Importantly, as compared with noncancerous tissues, a number of these significant changes were clearly detectable early on in stage I. Application of N-glycan data obtained from tissues was next assessed and validated for evaluation of small sized biopsies obtained via bronchoscopy. In summary, observed alterations and data of newly detected N-glycans expand knowledge about the glycosylation in LAC and may contribute to research in more tailored therapies. Moreover, the results demonstrate effectiveness of the presented approach for utility in rapid discrimination of cancerous from healthy lung tissues.
ER  -

LATTOVÁ, Erika; Jana SKŘIČKOVÁ; Jitka HAUSNEROVÁ; Lukáš FROLA; Leoš KŘEN; Ivana IHNATOVÁ; Zbyněk ZDRÁHAL; J. BRYANT a M. POPOVIC. N-Glycan profiling of lung adenocarcinoma in patients at different stages of disease. \textit{MODERN PATHOLOGY}. NEW YORK: NATURE PUBLISHING GROUP, 2020, roč.~33, č.~6, s.~1146-1156. ISSN~0893-3952. Dostupné z: https://doi.org/10.1038/s41379-019-0441-3.

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