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@proceedings{1683377, author = {Břenková, Barbora and Šiborová, Marta and Nováček, Jiří and Bárdy, Pavol and Pantůček, Roman and Plevka, Pavel}, booktitle = {XVI. Discussions in Structural Molecular Biology}, keywords = {S. aureus, connector, X-ray crystallography, cryo electron microscopy}, language = {eng}, title = {Structure of a staphylococcal phage connector protein}, url = {http://cssb.structbio.org/program-xvi-discussions-structural-molecular-biology}, year = {2019} }
TY - CONF ID - 1683377 AU - Břenková, Barbora - Šiborová, Marta - Nováček, Jiří - Bárdy, Pavol - Pantůček, Roman - Plevka, Pavel PY - 2019 TI - Structure of a staphylococcal phage connector protein KW - S. aureus, connector, X-ray crystallography, cryo electron microscopy UR - http://cssb.structbio.org/program-xvi-discussions-structural-molecular-biology N2 - Antibiotic resistance of microorganisms is a major threat accounting for a rising number of deaths. Bacteriophages offer an alternative to conventional antimicrobial treatment. Phage phi812 is a lytic virus from the Myoviridae family infecting up to 95 % of Staphylococcus aureus strains, including MRSA and its structure was previously determined by cryo-electron microscopy. Here we present our work towards resolving the structure of protein gp99 forming the connector region. The protein may play a role in genome release regulation during infection. Gp99 was overexpressed in E. coli and purified by affinity and size exclusion chromatography. A suitable crystallization condition was found and X-ray diffraction data with a resolution of 2.2 Å were collected. To solve the phase problem, a variety of methods had been tried, including molecular replacement, heavy atom soaking, co-crystallization, and seleno-methionine incorporation. The structure of gp99 will help explain the mechanism of bacterial infection by bacteriophage. ER -
BŘENKOVÁ, Barbora, Marta ŠIBOROVÁ, Jiří NOVÁČEK, Pavol BÁRDY, Roman PANTŮČEK a Pavel PLEVKA. Structure of a staphylococcal phage connector protein. In \textit{XVI. Discussions in Structural Molecular Biology}. 2019.
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