Lipoprotein Particles Interact with Membranes and Transfer
Their Cargo without Receptors

J 2020

Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors

PLOCHBERGER, B.; T. SYCH; F. WEBER; Jiří NOVÁČEK; M. AXMANN et al.

Základní údaje

Originální název

Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors

Autoři

PLOCHBERGER, B.; T. SYCH; F. WEBER; Jiří NOVÁČEK; M. AXMANN; H. STANGL a E. SEZGIN

Vydání

Biochemistry, Washington, American Chemical Society, 2020, 0006-2960

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.163

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/20:00118331

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

CHOLESTEROL

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:10, Ing. Martina Blahová

Anotace

V originále

Lipid transfer from lipoprotein particles to cells is essential for lipid homeostasis. High-density lipoprotein (HDL) particles are mainly captured by cell membrane-associated scavenger receptor class B type 1 (SR-B1) from the bloodstream, while low-density and very-low-density lipoprotein (LDL and VLDL, respectively) particles are mostly taken up by receptor-mediated endocytosis. However, the role of the target lipid membrane itself in the transfer process has been largely neglected so far. Here, we study how lipoprotein particles (HDL, LDL, and VLDL) interact with synthetic lipid bilayers and cell-derived membranes and transfer their cargo subsequently. Employing cryoelectron microscopy, spectral imaging, and fluorescence (cross correlation spectroscopy allowed us to observe integration of all major types of lipoprotein particles into the membrane and delivery of their cargo in a receptor-independent manner. Importantly, the biophysical properties of the target cell membranes change upon delivery of cargo. The concept of receptor-independent interaction of lipoprotein particles with membranes helps us to better understand lipoprotein particle biology and can be exploited for novel treatments of dyslipidemia diseases.

Návaznosti

90127, velká výzkumná infrastruktura

Název: CIISB II

Citovat

PLOCHBERGER, B.; T. SYCH; F. WEBER; Jiří NOVÁČEK; M. AXMANN; H. STANGL a E. SEZGIN. Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors. Biochemistry. Washington: American Chemical Society, 2020, roč. 59, č. 45, s. 4421-4428. ISSN 0006-2960. Dostupné z: https://doi.org/10.1021/acs.biochem.0c00748.

@article{1749407,
   author = {Plochberger, B. and Sych, T. and Weber, F. and Nováček, Jiří and Axmann, M. and Stangl, H. and Sezgin, E.},
   article_location = {Washington},
   article_number = {45},
   doi = {https://doi.org/10.1021/acs.biochem.0c00748},
   keywords = {CHOLESTEROL},
   language = {eng},
   issn = {0006-2960},
   journal = {Biochemistry},
   title = {Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors},
   url = {https://pubs.acs.org/doi/10.1021/acs.biochem.0c00748},
   volume = {59},
   year = {2020}
}

TY  - JOUR
ID  - 1749407
AU  - Plochberger, B. - Sych, T. - Weber, F. - Nováček, Jiří - Axmann, M. - Stangl, H. - Sezgin, E.
PY  - 2020
TI  - Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors
JF  - Biochemistry
VL  - 59
IS  - 45
SP  - 4421-4428
EP  - 4421-4428
PB  - American Chemical Society
SN  - 00062960
KW  - CHOLESTEROL
UR  - https://pubs.acs.org/doi/10.1021/acs.biochem.0c00748
N2  - Lipid transfer from lipoprotein particles to cells is essential for lipid homeostasis. High-density lipoprotein (HDL) particles are mainly captured by cell membrane-associated scavenger receptor class B type 1 (SR-B1) from the bloodstream, while low-density and very-low-density lipoprotein (LDL and VLDL, respectively) particles are mostly taken up by receptor-mediated endocytosis. However, the role of the target lipid membrane itself in the transfer process has been largely neglected so far. Here, we study how lipoprotein particles (HDL, LDL, and VLDL) interact with synthetic lipid bilayers and cell-derived membranes and transfer their cargo subsequently. Employing cryoelectron microscopy, spectral imaging, and fluorescence (cross correlation spectroscopy allowed us to observe integration of all major types of lipoprotein particles into the membrane and delivery of their cargo in a receptor-independent manner. Importantly, the biophysical properties of the target cell membranes change upon delivery of cargo. The concept of receptor-independent interaction of lipoprotein particles with membranes helps us to better understand lipoprotein particle biology and can be exploited for novel treatments of dyslipidemia diseases.
ER  -

PLOCHBERGER, B.; T. SYCH; F. WEBER; Jiří NOVÁČEK; M. AXMANN; H. STANGL a E. SEZGIN. Lipoprotein Particles Interact with Membranes and Transfer Their Cargo without Receptors. \textit{Biochemistry}. Washington: American Chemical Society, 2020, roč.~59, č.~45, s.~4421-4428. ISSN~0006-2960. Dostupné z: https://doi.org/10.1021/acs.biochem.0c00748.

Přehled o publikaci