In-cell NMR Spectroscopy of Nucleic Acids

J 2020

In-cell NMR Spectroscopy of Nucleic Acids

DŽATKO, Šimon; Radovan FIALA; R. HANSEL-HERTSCH; Silvie TRANTÍRKOVÁ; Lukáš TRANTÍREK et al.

Základní údaje

Originální název

In-cell NMR Spectroscopy of Nucleic Acids

Autoři

DŽATKO, Šimon; Radovan FIALA; R. HANSEL-HERTSCH; Silvie TRANTÍRKOVÁ a Lukáš TRANTÍREK

Vydání

IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY, CAMBRIDGE, ROYAL SOC CHEMISTRY, 2020, 2044-253X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/20:00118650

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

DNA; guanine quadruplex; nucleic acid; water

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2021 19:08, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

The structure, dynamics, and interactions of nucleic acids, in particular DNAs, sensitively respond to a broad range of environmental factors, many of them being natively present in the intracellular space. In-cell NMR is a tailored adaptation of conventional high-resolution nuclear magnetic resonance spectroscopy that permits characterization of stereochemical features of nucleic acids directly in living cells. Here we review recent advances and applications of in-cell NMR to nucleic acids. The limitations of this method as well as the general considerations, comprising of critical factors to be accounted for during the planning of in-cell NMR experiments, as well as during the interpretation of in-cell NMR data, are discussed.

Návaznosti

NV19-08-00450, projekt VaV

Název: Atomárně rozlišená NMR spektroskopie in vivo jako nástroj pro biologické testování terapeuticky významných cílů v genomové ne-kanonické DNA a jejich interakcí s léčivy ve fenotypově diverzifikovaných nádorových buňkách.

Investor: Ministerstvo zdravotnictví ČR, Atomically resolved in vivo NMR spectroscopy as a novel tool for biological testing of therapeutically important genomic non-canonical DNA targets and their interactions with drugs in phenotypically diversified cancer cells.

Citovat

DŽATKO, Šimon; Radovan FIALA; R. HANSEL-HERTSCH; Silvie TRANTÍRKOVÁ a Lukáš TRANTÍREK. In-cell NMR Spectroscopy of Nucleic Acids. IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY. CAMBRIDGE: ROYAL SOC CHEMISTRY, 2020, roč. 21, č. 2020, s. 272-297. ISSN 2044-253X. Dostupné z: https://doi.org/10.1039/9781788013079.

@article{1752481,
   author = {Džatko, Šimon and Fiala, Radovan and HanselandHertsch, R. and Trantírková, Silvie and Trantírek, Lukáš},
   article_location = {CAMBRIDGE},
   article_number = {2020},
   doi = {https://doi.org/10.1039/9781788013079},
   keywords = {DNA; guanine quadruplex; nucleic acid; water},
   language = {eng},
   issn = {2044-253X},
   journal = {IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY},
   title = {In-cell NMR Spectroscopy of Nucleic Acids},
   url = {https://pubs.rsc.org/en/content/chapter/bk9781788012171-00272/978-1-78801-217-1},
   volume = {21},
   year = {2020}
}

TY  - JOUR
ID  - 1752481
AU  - Džatko, Šimon - Fiala, Radovan - Hansel-Hertsch, R. - Trantírková, Silvie - Trantírek, Lukáš
PY  - 2020
TI  - In-cell NMR Spectroscopy of Nucleic Acids
JF  - IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY
VL  - 21
IS  - 2020
SP  - 272-297
EP  - 272-297
PB  - ROYAL SOC CHEMISTRY
SN  - 2044253X
KW  - DNA
KW  - guanine quadruplex
KW  - nucleic acid
KW  - water
UR  - https://pubs.rsc.org/en/content/chapter/bk9781788012171-00272/978-1-78801-217-1
N2  - The structure, dynamics, and interactions of nucleic acids, in particular DNAs, sensitively respond to a broad range of environmental factors, many of them being natively present in the intracellular space. In-cell NMR is a tailored adaptation of conventional high-resolution nuclear magnetic resonance spectroscopy that permits characterization of stereochemical features of nucleic acids directly in living cells. Here we review recent advances and applications of in-cell NMR to nucleic acids. The limitations of this method as well as the general considerations, comprising of critical factors to be accounted for during the planning of in-cell NMR experiments, as well as during the interpretation of in-cell NMR data, are discussed.
ER  -

DŽATKO, Šimon; Radovan FIALA; R. HANSEL-HERTSCH; Silvie TRANTÍRKOVÁ a Lukáš TRANTÍREK. In-cell NMR Spectroscopy of Nucleic Acids. \textit{IN-CELL NMR SPECTROSCOPY: FROM MOLECULAR SCIENCES TO CELL BIOLOGY}. CAMBRIDGE: ROYAL SOC CHEMISTRY, 2020, roč.~21, č.~2020, s.~272-297. ISSN~2044-253X. Dostupné z: https://doi.org/10.1039/9781788013079.

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