c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway
in breast cancer cells

J 2021

c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway in breast cancer cells

DÚCKA, Monika; Martina KUČERÍKOVÁ; Filip TRČKA; Jakub ČERVINKA; Elisabetta BIGLIERI et al.

Základní údaje

Originální název

c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway in breast cancer cells

Autoři

DÚCKA, Monika; Martina KUČERÍKOVÁ; Filip TRČKA; Jakub ČERVINKA; Elisabetta BIGLIERI; Jan ŠMARDA; Lubor BORSIG; Petr BENEŠ a Lucia KNOPFOVÁ

Vydání

Neoplasia, New York, Elsevier Inc. 2021, 1476-5586

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10603 Genetics and heredity

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 6.218

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/21:00118896

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Breast cancer; c-Myb; IL1alpha; NF-kappaB; Inflammation; Transactivation

Štítky

14314010, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 4. 2022 09:15, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The transcription factor c-Myb can be involved in the activation of many genes with protumorigenic function; however, its role in breast cancer (BC) development is still under discussion. c-Myb is considered as a tumor-promoting factor in the early phases of BC, on the other hand, its expression in BC patients relates to a good prognosis. Previously, we have shown that c-Myb controls the capacity of BC cells to form spontaneous lung metastasis. Reduced seeding of BC cells to the lungs is linked to high expression of c-Myb and a decline in expression of a specific set of inflammatory genes. Here, we unraveled a c-Myb-IL1alpha-NF-kappaB signaling axis that takes place in tumor cells. We report that an overexpression of c-Myb interfered with the activity of NF-kappaB in several BC cell lines. We identified IL1alpha to be essential for this interference since it was abrogated in the IL1alpha-deficient cells. Overexpression of IL1alpha, as well as addition of recombinant IL1alpha protein, activated NF-kappaB signaling and restored expression of the inflammatory signature genes suppressed by c-Myb. The endogenous levels of c-Myb negatively correlated with IL1alpha on both transcriptional and protein levels across BC cell lines. We concluded that inhibition of IL1alpha expression by c-Myb reduces NF-kappaB activity and disconnects the inflammatory circuit, a potentially targetable mechanism to mimic the antimetastatic effect of c-Myb with therapeutic perspective.

Návaznosti

GJ17-08985Y, projekt VaV

Název: Prozánětlivá signalizace pod kontrolou proteinu c-Myb v bazálních prsních karcinomech

Investor: Grantová agentura ČR, c-Myb-regulated inflammatory circuit in basal-like mammary tumors

NV18-07-00073, projekt VaV

Název: c-Myb a jeho transkripční program ve fyziologických a patologických osteogenních procesech

Investor: Ministerstvo zdravotnictví ČR, c-Myb a jeho transkripční program ve fyziologických a patologických osteogenních procesech

Citovat

DÚCKA, Monika; Martina KUČERÍKOVÁ; Filip TRČKA; Jakub ČERVINKA; Elisabetta BIGLIERI; Jan ŠMARDA; Lubor BORSIG; Petr BENEŠ a Lucia KNOPFOVÁ. c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway in breast cancer cells. Neoplasia. New York: Elsevier Inc., 2021, roč. 23, č. 3, s. 326-336. ISSN 1476-5586. Dostupné z: https://doi.org/10.1016/j.neo.2021.01.002.

@article{1753458,
   author = {Dúcka, Monika and Kučeríková, Martina and Trčka, Filip and Červinka, Jakub and Biglieri, Elisabetta and Šmarda, Jan and Borsig, Lubor and Beneš, Petr and Knopfová, Lucia},
   article_location = {New York},
   article_number = {3},
   doi = {https://doi.org/10.1016/j.neo.2021.01.002},
   keywords = {Breast cancer; c-Myb; IL1alpha; NF-kappaB; Inflammation; Transactivation},
   language = {eng},
   issn = {1476-5586},
   journal = {Neoplasia},
   title = {c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway in breast cancer cells},
   url = {https://doi.org/10.1016/j.neo.2021.01.002},
   volume = {23},
   year = {2021}
}

TY  - JOUR
ID  - 1753458
AU  - Dúcka, Monika - Kučeríková, Martina - Trčka, Filip - Červinka, Jakub - Biglieri, Elisabetta - Šmarda, Jan - Borsig, Lubor - Beneš, Petr - Knopfová, Lucia
PY  - 2021
TI  - c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway in breast cancer cells
JF  - Neoplasia
VL  - 23
IS  - 3
SP  - 326-336
EP  - 326-336
PB  - Elsevier Inc.
SN  - 14765586
KW  - Breast cancer
KW  - c-Myb
KW  - IL1alpha
KW  - NF-kappaB
KW  - Inflammation
KW  - Transactivation
UR  - https://doi.org/10.1016/j.neo.2021.01.002
N2  - The transcription factor c-Myb can be involved in the activation of many genes with protumorigenic function; however, its role in breast cancer (BC) development is still under discussion. c-Myb is considered as a tumor-promoting factor in the early phases of BC, on the other hand, its expression in BC patients relates to a good prognosis. Previously, we have shown that c-Myb controls the capacity of BC cells to form spontaneous lung metastasis. Reduced seeding of BC cells to the lungs is linked to high expression of c-Myb and a decline in expression of a specific set of inflammatory genes. Here, we unraveled a c-Myb-IL1alpha-NF-kappaB signaling axis that takes place in tumor cells. We report that an overexpression of c-Myb interfered with the activity of NF-kappaB in several BC cell lines. We identified IL1alpha to be essential for this interference since it was abrogated in the IL1alpha-deficient cells. Overexpression of IL1alpha, as well as addition of recombinant IL1alpha protein, activated NF-kappaB signaling and restored expression of the inflammatory signature genes suppressed by c-Myb. The endogenous levels of c-Myb negatively correlated with IL1alpha on both transcriptional and protein levels across BC cell lines. We concluded that inhibition of IL1alpha expression by c-Myb reduces NF-kappaB activity and disconnects the inflammatory circuit, a potentially targetable mechanism to mimic the antimetastatic effect of c-Myb with therapeutic perspective.
ER  -

DÚCKA, Monika; Martina KUČERÍKOVÁ; Filip TRČKA; Jakub ČERVINKA; Elisabetta BIGLIERI; Jan ŠMARDA; Lubor BORSIG; Petr BENEŠ a Lucia KNOPFOVÁ. c-Myb interferes with inflammatory IL1alpha-NF-kappaB pathway in breast cancer cells. \textit{Neoplasia}. New York: Elsevier Inc., 2021, roč.~23, č.~3, s.~326-336. ISSN~1476-5586. Dostupné z: https://doi.org/10.1016/j.neo.2021.01.002.

Přehled o publikaci