J 1995

Characterization of Matrix-Assisted Laser Desorption Based on Absorption and Acoustic Monitoring

PREISLER, J. a E. S. YEUNG

Základní údaje

Originální název

Characterization of Matrix-Assisted Laser Desorption Based on Absorption and Acoustic Monitoring

Autoři

PREISLER, J. (203 Česká republika, garant) a E. S. YEUNG (840 Spojené státy)

Vydání

Applied Spectroscopy, USA, Society Appl. Spectroscopy, 1995, 0003-7028

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Organizační jednotka

Přírodovědecká fakulta

UT WoS

A1995TM58600021

Klíčová slova anglicky

MALDI; desorption; laser; acoustic; photoacoustic; deflect

Štítky

Změněno: 28. 6. 2009 00:05, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

Conventional methods for studying matrix-assisted desorption/ionization rely on mass spectroscopy. In this research, a 488-nm argon-ion laser beam is deflected by two acoustooptic deflectors to image plumes desorbed at atmospheric pressure via absorption. All species, including neutral molecules, are monitored. Interesting features, e.g. differences between the initial plume and subsequent plumes desorbed from the same spot, or the formation of two plumes from one laser shot are observed. Total plume absorbance can be correlated with the acoustic signal generated by the desorption event. A model equation for the plume velocity as a function of time is proposed. Optical probing also enables accurate determination of plume velocities at reduced pressures. These results define the optimal conditions for desorbing analytes from matrices, as opposed to achieving a compromise between efficient desorption and efficient ionization as is practiced in mass spectrometry.