Lack of Association between Epidermal Growth Factor or Its
Receptor and Reflux Esophagitis, Barrett’s Esophagus, and
Esophageal Adenocarcinoma: A Case-Control Study

J 2022

Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett’s Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study

DEISSOVÁ, Tereza; Michaela CVANOVÁ; Zdeněk KALA; Zuzana JIRASKOVA ZAKOSTELSKA; Jiří DOLINA et al.

Základní údaje

Originální název

Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett’s Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study

Autoři

Vydání

Disease Markers, LONDON, HINDAWI LTD, 2022, 0278-0240

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30101 Human genetics

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.464 v roce 2021

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/22:00126886

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Epidermal Growth Factor; Reflux Esophagitis; Barrett's Esophagus; Esophageal Adenocarcinoma

Štítky

14110130, 14110213, 14110223, 14110227, 14110229, 14110230, 14110513, 14110518, 14119612, 143180, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 6. 2025 11:19, Mgr. Michaela Hylsová, Ph.D.

Anotace

V originále

The epidermal growth factor (EGF) and its receptor (EGFR) gene-gene interactions were shown to increase the susceptibility to esophageal cancer. However, the role of the EGF/EGFR pathway in the development of gastroesophageal reflux disease (GERD) and its complications (reflux esophagitis (RE), Barrett’s esophagus (BE), and esophageal adenocarcinoma (EAC)) remains unclear. This association study is aimed at investigating functional EGF and EGFR gene polymorphisms, their mRNA expression in esophageal tissues, and EGF plasma levels in relation to RE, BE, and EAC development in the Central European population. 301 patients with RE/BE/EAC (cases) as well as 98 patients with nonerosive reflux disease (NERD) and 8 healthy individuals (controls) were genotyped for +61 A>G EGF (rs4444903) and +142285 G>A EGFR (rs2227983) polymorphisms using the TaqMan quantitative polymerase chain reaction (qPCR). In random subgroups, the EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in esophageal tissue with and without endoscopically visible pathological changes; and the EGF plasma levels were determined by enzyme-linked immunosorbent assay. None of the genotyped SNPs nor EGF-EGFR genotype interactions were associated with RE, BE, or EAC development (). Moreover, mRNA expression of neither EGF nor EGFR differed between samples of the esophageal tissue with and without endoscopically visible pathology () nor between samples from patients with different diagnoses, i.e., RE, BE, or EAC (). Nevertheless, the lower EGF mRNA expression in carriers of combined genotypes AA +61 EGF (rs4444903) and GG +142285 EGFR (rs2227983; ) suggests a possible direct/indirect effect of EGF-EGFR gene interactions on EGF gene expression. In conclusion, EGF and EGFR gene variants and their mRNA/protein expression were not associated with RE, BE or EAC development in the Central European population.

Návaznosti

EF17_043/0009632, projekt VaV

Název: CETOCOEN Excellence

NU20-03-00126, projekt VaV

Název: Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu

Investor: Ministerstvo zdravotnictví ČR, Hostitelský mikrobiom ve vztahu k rozvoji Barrettova jícnu a adenokarcinomu jícnu, Podprogram 1 - standardní

857560, interní kód MU
(Kód CEP: EF17_043/0009632)

Název: CETOCOEN Excellence (Akronym: CETOCOEN Excellence)

Investor: Evropská unie, CETOCOEN Excellence, Spreading excellence and widening participation

90121, velká výzkumná infrastruktura

Název: RECETOX RI

Přiložené soubory

2224704.pdf

Citovat

DEISSOVÁ, Tereza; Michaela CVANOVÁ; Zdeněk KALA; Zuzana JIRASKOVA ZAKOSTELSKA; Jiří DOLINA; Lumír KUNOVSKÝ; Radek KROUPA; Zdeněk PAVLOVSKÝ; Břetislav LIPOVÝ; Zdeněk DANĚK; Lydie IZAKOVIČOVÁ HOLLÁ; Ondrej URBAN; Vit NAVRATIL; Robert LISCHKE; Tomas HARUSTIAK; Tomáš GROLICH; Vladimír PROCHÁZKA; Ondřej SLABÝ a Petra BOŘILOVÁ LINHARTOVÁ. Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett’s Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study. Disease Markers. LONDON: HINDAWI LTD, 2022, roč. 2022, August, s. 1-13. ISSN 0278-0240. Dostupné z: https://doi.org/10.1155/2022/8790748.

@article{2224704,
   author = {Deissová, Tereza and Cvanová, Michaela and Kala, Zdeněk and Jiraskova Zakostelska, Zuzana and Dolina, Jiří and Kunovský, Lumír and Kroupa, Radek and Pavlovský, Zdeněk and Lipový, Břetislav and Daněk, Zdeněk and Izakovičová Hollá, Lydie and Urban, Ondrej and Navratil, Vit and Lischke, Robert and Harustiak, Tomas and Grolich, Tomáš and Procházka, Vladimír and Slabý, Ondřej and Bořilová Linhartová, Petra},
   article_location = {LONDON},
   article_number = {August},
   doi = {https://doi.org/10.1155/2022/8790748},
   keywords = {Epidermal Growth Factor; Reflux Esophagitis; Barrett's Esophagus; Esophageal Adenocarcinoma},
   language = {eng},
   issn = {0278-0240},
   journal = {Disease Markers},
   title = {Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett’s Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study},
   url = {https://www.hindawi.com/journals/dm/2022/8790748/},
   volume = {2022},
   year = {2022}
}

TY  - JOUR
ID  - 2224704
AU  - Deissová, Tereza - Cvanová, Michaela - Kala, Zdeněk - Jiraskova Zakostelska, Zuzana - Dolina, Jiří - Kunovský, Lumír - Kroupa, Radek - Pavlovský, Zdeněk - Lipový, Břetislav - Daněk, Zdeněk - Izakovičová Hollá, Lydie - Urban, Ondrej - Navratil, Vit - Lischke, Robert - Harustiak, Tomas - Grolich, Tomáš - Procházka, Vladimír - Slabý, Ondřej - Bořilová Linhartová, Petra
PY  - 2022
TI  - Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett’s Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study
JF  - Disease Markers
VL  - 2022
IS  - August
SP  - 1-13
EP  - 1-13
PB  - HINDAWI LTD
SN  - 02780240
KW  - Epidermal Growth Factor
KW  - Reflux Esophagitis
KW  - Barrett's Esophagus
KW  - Esophageal Adenocarcinoma
UR  - https://www.hindawi.com/journals/dm/2022/8790748/
N2  - The epidermal growth factor (EGF) and its receptor (EGFR) gene-gene interactions were shown to increase the susceptibility to esophageal cancer. However, the role of the EGF/EGFR pathway in the development of gastroesophageal reflux disease (GERD) and its complications (reflux esophagitis (RE), Barrett’s esophagus (BE), and esophageal adenocarcinoma (EAC)) remains unclear. This association study is aimed at investigating functional EGF and EGFR gene polymorphisms, their mRNA expression in esophageal tissues, and EGF plasma levels in relation to RE, BE, and EAC development in the Central European population. 301 patients with RE/BE/EAC (cases) as well as 98 patients with nonerosive reflux disease (NERD) and 8 healthy individuals (controls) were genotyped for +61 A>G EGF (rs4444903) and +142285 G>A EGFR (rs2227983) polymorphisms using the TaqMan quantitative polymerase chain reaction (qPCR). In random subgroups, the EGF and EGFR mRNA expressions were analyzed by reverse transcription qPCR in esophageal tissue with and without endoscopically visible pathological changes; and the EGF plasma levels were determined by enzyme-linked immunosorbent assay. None of the genotyped SNPs nor EGF-EGFR genotype interactions were associated with RE, BE, or EAC development (). Moreover, mRNA expression of neither EGF nor EGFR differed between samples of the esophageal tissue with and without endoscopically visible pathology () nor between samples from patients with different diagnoses, i.e., RE, BE, or EAC (). Nevertheless, the lower EGF mRNA expression in carriers of combined genotypes AA +61 EGF (rs4444903) and GG +142285 EGFR (rs2227983; ) suggests a possible direct/indirect effect of EGF-EGFR gene interactions on EGF gene expression. In conclusion, EGF and EGFR gene variants and their mRNA/protein expression were not associated with RE, BE or EAC development in the Central European population.
ER  -

DEISSOVÁ, Tereza; Michaela CVANOVÁ; Zdeněk KALA; Zuzana JIRASKOVA ZAKOSTELSKA; Jiří DOLINA; Lumír KUNOVSKÝ; Radek KROUPA; Zdeněk PAVLOVSKÝ; Břetislav LIPOVÝ; Zdeněk DANĚK; Lydie IZAKOVIČOVÁ HOLLÁ; Ondrej URBAN; Vit NAVRATIL; Robert LISCHKE; Tomas HARUSTIAK; Tomáš GROLICH; Vladimír PROCHÁZKA; Ondřej SLABÝ a Petra BOŘILOVÁ LINHARTOVÁ. Lack of Association between Epidermal Growth Factor or Its Receptor and Reflux Esophagitis, Barrett’s Esophagus, and Esophageal Adenocarcinoma: A Case-Control Study. \textit{Disease Markers}. LONDON: HINDAWI LTD, 2022, roč.~2022, August, s.~1-13. ISSN~0278-0240. Dostupné z: https://doi.org/10.1155/2022/8790748.

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