Tail proteins of phage SU10 reorganize into the nozzle for
genome delivery

J 2022

Tail proteins of phage SU10 reorganize into the nozzle for genome delivery

ŠIBOROVÁ, Marta; Tibor FÜZIK; Michaela PROCHÁZKOVÁ; Jiří NOVÁČEK; Martin BENEŠÍK et al.

Základní údaje

Originální název

Tail proteins of phage SU10 reorganize into the nozzle for genome delivery

Autoři

ŠIBOROVÁ, Marta; Tibor FÜZIK ; Michaela PROCHÁZKOVÁ ; Jiří NOVÁČEK; Martin BENEŠÍK; Anders S NILSSON a Pavel PLEVKA

Vydání

Nature Communications, London, Nature Publishing Group, 2022, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10607 Virology

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.600

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/22:00127053

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Bacteriophages; DNA; Viral; Genome; Phosmet; Podoviridae

Štítky

CF CELLIM, CF CRYO, CF PROT, NIVB_CEITEC, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 10. 2024 14:26, Ing. Martina Blahová

Anotace

V originále

E. coli phage SU10 has a short non-contractile tail. Here, the authors show that after cell binding, nozzle proteins and tail fibers of SU10 change conformation to form a nozzle that enables the delivery of the phage DNA into the bacterial cytoplasm. Escherichia coli phage SU10 belongs to the genus Kuravirus from the class Caudoviricetes of phages with short non-contractile tails. In contrast to other short-tailed phages, the tails of Kuraviruses elongate upon cell attachment. Here we show that the virion of SU10 has a prolate head, containing genome and ejection proteins, and a tail, which is formed of portal, adaptor, nozzle, and tail needle proteins and decorated with long and short fibers. The binding of the long tail fibers to the receptors in the outer bacterial membrane induces the straightening of nozzle proteins and rotation of short tail fibers. After the re-arrangement, the nozzle proteins and short tail fibers alternate to form a nozzle that extends the tail by 28 nm. Subsequently, the tail needle detaches from the nozzle proteins and five types of ejection proteins are released from the SU10 head. The nozzle with the putative extension formed by the ejection proteins enables the delivery of the SU10 genome into the bacterial cytoplasm. It is likely that this mechanism of genome delivery, involving the formation of the tail nozzle, is employed by all Kuraviruses.

Návaznosti

GA18-17810S, projekt VaV

Název: Strukturní analýza replikace fága v buňkách zlatého stafylokoka

Investor: Grantová agentura ČR, In vivo strukturní analýza replikace fága infikujícího zlatého stafylokoka

LL1906, projekt VaV

Název: Replikace fágů v bakteriálním biofilmu

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Replikace fágů v bakteriálním biofilmu

LM2010005, projekt VaV

Název: Velká infrastruktura CESNET (Akronym: VI CESNET)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Velká infrastruktura CESNET

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

90127, velká výzkumná infrastruktura

Název: CIISB II

90129, velká výzkumná infrastruktura

Název: Czech-BioImaging II

Přiložené soubory

s41467-022-33305-w.pdf Verze souboru

Citovat

ŠIBOROVÁ, Marta; Tibor FÜZIK; Michaela PROCHÁZKOVÁ; Jiří NOVÁČEK; Martin BENEŠÍK; Anders S NILSSON a Pavel PLEVKA. Tail proteins of phage SU10 reorganize into the nozzle for genome delivery. Nature Communications. London: Nature Publishing Group, 2022, roč. 13, č. 1, s. 5622-5634. ISSN 2041-1723. Dostupné z: https://doi.org/10.1038/s41467-022-33305-w.

@article{2228337,
   author = {Šiborová, Marta and Füzik, Tibor and Procházková, Michaela and Nováček, Jiří and Benešík, Martin and Nilsson, Anders S and Plevka, Pavel},
   article_location = {London},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41467-022-33305-w},
   keywords = {Bacteriophages; DNA; Viral; Genome; Phosmet; Podoviridae},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Tail proteins of phage SU10 reorganize into the nozzle for genome delivery},
   url = {https://www.nature.com/articles/s41467-022-33305-w},
   volume = {13},
   year = {2022}
}

TY  - JOUR
ID  - 2228337
AU  - Šiborová, Marta - Füzik, Tibor - Procházková, Michaela - Nováček, Jiří - Benešík, Martin - Nilsson, Anders S - Plevka, Pavel
PY  - 2022
TI  - Tail proteins of phage SU10 reorganize into the nozzle for genome delivery
JF  - Nature Communications
VL  - 13
IS  - 1
SP  - 5622
EP  - 5622
PB  - Nature Publishing Group
SN  - 20411723
KW  - Bacteriophages
KW  - DNA
KW  - Viral
KW  - Genome
KW  - Phosmet
KW  - Podoviridae
UR  - https://www.nature.com/articles/s41467-022-33305-w
N2  - E. coli phage SU10 has a short non-contractile tail. Here, the authors show that after cell binding, nozzle proteins and tail fibers of SU10 change conformation to form a nozzle that enables the delivery of the phage DNA into the bacterial cytoplasm. Escherichia coli phage SU10 belongs to the genus Kuravirus from the class Caudoviricetes of phages with short non-contractile tails. In contrast to other short-tailed phages, the tails of Kuraviruses elongate upon cell attachment. Here we show that the virion of SU10 has a prolate head, containing genome and ejection proteins, and a tail, which is formed of portal, adaptor, nozzle, and tail needle proteins and decorated with long and short fibers. The binding of the long tail fibers to the receptors in the outer bacterial membrane induces the straightening of nozzle proteins and rotation of short tail fibers. After the re-arrangement, the nozzle proteins and short tail fibers alternate to form a nozzle that extends the tail by 28 nm. Subsequently, the tail needle detaches from the nozzle proteins and five types of ejection proteins are released from the SU10 head. The nozzle with the putative extension formed by the ejection proteins enables the delivery of the SU10 genome into the bacterial cytoplasm. It is likely that this mechanism of genome delivery, involving the formation of the tail nozzle, is employed by all Kuraviruses.
ER  -

ŠIBOROVÁ, Marta; Tibor FÜZIK; Michaela PROCHÁZKOVÁ; Jiří NOVÁČEK; Martin BENEŠÍK; Anders S NILSSON a Pavel PLEVKA. Tail proteins of phage SU10 reorganize into the nozzle for genome delivery. \textit{Nature Communications}. London: Nature Publishing Group, 2022, roč.~13, č.~1, s.~5622-5634. ISSN~2041-1723. Dostupné z: https://doi.org/10.1038/s41467-022-33305-w.

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