Concentration and diffusion of the redox probe as key
parameters for label-free impedimetric immunosensing

J 2023

Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing

LACINA, Karel, Jakub VĚŽNÍK, Jakub SOPOUŠEK, Zdeněk FARKA, Veronika LACINOVÁ et. al.

Basic information

Original name

Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing

Authors

LACINA, Karel (203 Czech Republic, guarantor, belonging to the institution), Jakub VĚŽNÍK (203 Czech Republic, belonging to the institution), Jakub SOPOUŠEK (203 Czech Republic, belonging to the institution), Zdeněk FARKA (203 Czech Republic, belonging to the institution), Veronika LACINOVÁ (203 Czech Republic) and Petr SKLÁDAL (203 Czech Republic, belonging to the institution)

Edition

Bioelectrochemistry, Elsevier, 2023, 1567-5394

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10405 Electrochemistry

Country of publisher

Netherlands

Confidentiality degree

není předmětem státního či obchodního tajemství

References:

URL

Impact factor

Impact factor: 5.000 in 2022

RIV identification code

RIV/00216224:14310/23:00130058

Organization unit

Faculty of Science

DOI

http://dx.doi.org/10.1016/j.bioelechem.2022.108308

UT WoS

000890307800001

Keywords in English

Electrochemical biosensor Electrochemical impedance spectroscopy Immunosensor Diffusion layer Nanopore Polystyrene nanoparticle

Abstract

V originále

Nanoporous surfaces are promising for label-free electrochemical biosensing. We formed nanopores directly on the electrode surface by means of assembling a dense layer of nonconductive nanoparticles. In our model affinity biosensor, covalent attachment of albumin protein on top of 40 nm polystyrene nanoparticles represented a capture of an analyte, resulting in blockage of the nanopores. Different bulk concentrations of the ferro/ferricyanide redox pair were probed by Faradaic electrochemical impedance spectroscopy and fast chronoamperometry. The character of the redox probe permeation towards the electrode surface differed in dependence on its concentration. These data were compared with the theoretical behavior of the free diffusion according to the Cottrell equation. Both the bulk concentration of the redox probe and the timescale of the experiment affected the performance of the electrochemical detection, demonstrating the importance of controlling these parameters in immunosensing applications.

Links

GA21-03156S, research and development project

Name: Foton-upkonverzní značky pro mikrofluidní jednomolekulové imunostanovení proteinových biomarkerů

Investor: Czech Science Foundation

LQ1601, research and development project

Name: CEITEC 2020 (Acronym: CEITEC2020)

Investor: Ministry of Education, Youth and Sports of the CR

NU20-08-00106, research and development project

Name: Elektrochemický imunodetektor pro detekci HMGB1 v séru pacientů s různými formami leukémie: metoda pro rychlé zhodnocení úspěšnosti léčby

Investor: Ministry of Health of the CR, Subprogram 1 - standard

19-16273Y, interní kód MU

Name: ROS-Induced Drug Release Based on Interactions of Ferrocenylboronates

Citovat

LACINA, Karel, Jakub VĚŽNÍK, Jakub SOPOUŠEK, Zdeněk FARKA, Veronika LACINOVÁ and Petr SKLÁDAL. Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing. Bioelectrochemistry. Elsevier, 2023, vol. 149, February 2023, p. 1-6. ISSN 1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2022.108308.

@article{2230161,
   author = {Lacina, Karel and Věžník, Jakub and Sopoušek, Jakub and Farka, Zdeněk and Lacinová, Veronika and Skládal, Petr},
   article_number = {February 2023},
   doi = {http://dx.doi.org/10.1016/j.bioelechem.2022.108308},
   keywords = {Electrochemical biosensor Electrochemical impedance spectroscopy Immunosensor Diffusion layer Nanopore Polystyrene nanoparticle},
   language = {eng},
   issn = {1567-5394},
   journal = {Bioelectrochemistry},
   title = {Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing},
   url = {https://www.sciencedirect.com/science/article/pii/S1567539422002596?via%3Dihub},
   volume = {149},
   year = {2023}
}

TY  - JOUR
ID  - 2230161
AU  - Lacina, Karel - Věžník, Jakub - Sopoušek, Jakub - Farka, Zdeněk - Lacinová, Veronika - Skládal, Petr
PY  - 2023
TI  - Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing
JF  - Bioelectrochemistry
VL  - 149
IS  - February 2023
SP  - 1-6
EP  - 1-6
PB  - Elsevier
SN  - 15675394
KW  - Electrochemical biosensor Electrochemical impedance spectroscopy Immunosensor Diffusion layer Nanopore Polystyrene nanoparticle
UR  - https://www.sciencedirect.com/science/article/pii/S1567539422002596?via%3Dihub
N2  - Nanoporous surfaces are promising for label-free electrochemical biosensing. We formed nanopores directly on the electrode surface by means of assembling a dense layer of nonconductive nanoparticles. In our model affinity biosensor, covalent attachment of albumin protein on top of 40 nm polystyrene nanoparticles represented a capture of an analyte, resulting in blockage of the nanopores. Different bulk concentrations of the ferro/ferricyanide redox pair were probed by Faradaic electrochemical impedance spectroscopy and fast chronoamperometry. The character of the redox probe permeation towards the electrode surface differed in dependence on its concentration. These data were compared with the theoretical behavior of the free diffusion according to the Cottrell equation. Both the bulk concentration of the redox probe and the timescale of the experiment affected the performance of the electrochemical detection, demonstrating the importance of controlling these parameters in immunosensing applications.
ER  -

LACINA, Karel, Jakub VĚŽNÍK, Jakub SOPOUŠEK, Zdeněk FARKA, Veronika LACINOVÁ and Petr SKLÁDAL. Concentration and diffusion of the redox probe as key parameters for label-free impedimetric immunosensing. \textit{Bioelectrochemistry}. Elsevier, 2023, vol.~149, February 2023, p.~1-6. ISSN~1567-5394. Available from: https://dx.doi.org/10.1016/j.bioelechem.2022.108308.

Detailed Information on Publication Record