1999
Chromosomes participating in translocations typical of malignant hemoblastoses are also involved in exchange aberrations induced by fast neutrons
LUKÁŠOVÁ, Emilie; Stanislav KOZUBEK; Michal KOZUBEK; Václav KROHA; Andrea MAREČKOVÁ et al.Základní údaje
Originální název
Chromosomes participating in translocations typical of malignant hemoblastoses are also involved in exchange aberrations induced by fast neutrons
Autoři
LUKÁŠOVÁ, Emilie; Stanislav KOZUBEK; Michal KOZUBEK; Václav KROHA; Andrea MAREČKOVÁ; Magdalena SKALNÍKOVÁ; Eva BÁRTOVÁ a Jana ŠLOTOVÁ
Vydání
Radiation Research, 1999, 0033-7587
Další údaje
Jazyk
angličtina
Typ výsledku
Článek v odborném periodiku
Obor
10610 Biophysics
Stát vydavatele
Spojené státy
Utajení
není předmětem státního či obchodního tajemství
Impakt faktor
Impact factor: 2.807
Označené pro přenos do RIV
Ano
Kód RIV
RIV/00216224:14330/99:00001456
Organizační jednotka
Fakulta informatiky
UT WoS
Klíčová slova anglicky
aberration induction; ionizing radiation; neutrons; nuclear topography; haemoblastoses
Příznaky
Mezinárodní význam, Recenzováno
Změněno: 7. 5. 2010 17:13, prof. RNDr. Michal Kozubek, Ph.D.
Anotace
V originále
Repeated triple-color fluorescence in situ hybridization was used for the detection of exchange aberrations among 10 selected chromosomes of human lymphocytes irradiated with three doses of fast neutrons with a mean energy of 7 MeV. In each hybridization two different pairs of chromosomes were stained. Defined stage positions of metaphases on a slide were stored on a hard disk and an automatic scan of images according to these positions was performed after six successive hybridizations. In this way we obtained six different images of the same metaphase with differently stained pairs of chromosomes and centromeres. The comparison of these images enabled the identification of mutual exchanges between chromosomes 1,2,3,4,8,9,12,14,18 and 22. The frequencies of exchanges were not linearly proportional to the molecular weight of interacting chromosomes. The most significant were exchanges between chromosomes 14/18, 14/8, 18/8, 8/3, 1/14, 1/8, 3/18, 3/14 and 9/22. The results indicate significant interactions between chromosomes involved in translocations in B-cell non-Hodgkin's lymphoma and chronic myeloid leukemia. We propose that the reason for the high frequency of exchanges between these chromosomes is their proximity in the cell nucleus. It may also be one of the reasons for the induction of specific translocations leading to the malignant transformation of cells.
Návaznosti
| GA202/96/1718, projekt VaV |
| ||
| MSM 143300002, záměr |
| ||
| NM15, projekt VaV |
| ||
| VS97031, projekt VaV |
|