| HENDRYCH, Michal, Kamila ŘÍHOVÁ, Barbora ADAMOVÁ, Vojtěch HRADIL, Marek STIBOREK, Petr VLČEK, Markéta HERMANOVÁ, Jana VAŠÍČKOVÁ, Petr BENEŠ, Jan ŠMARDA, Viktor KANICKÝ, Jan PREISLER a Jarmila NAVRÁTILOVÁ. Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma. BIOMEDICINE & PHARMACOTHERAPY. Paris: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2022, roč. 153, September 2022, s. 1-15. ISSN 0753-3322. Dostupné z: https://dx.doi.org/10.1016/j.biopha.2022.113465. |
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@article{2240067,
author = {Hendrych, Michal and Říhová, Kamila and Adamová, Barbora and Hradil, Vojtěch and Stiborek, Marek and Vlček, Petr and Hermanová, Markéta and Vašíčková, Jana and Beneš, Petr and Šmarda, Jan and Kanický, Viktor and Preisler, Jan and Navrátilová, Jarmila},
article_location = {Paris},
article_number = {September 2022},
doi = {http://dx.doi.org/10.1016/j.biopha.2022.113465},
keywords = {Disulfiram/copper; 5-fluorouracil; Synergism; Organotypic culture; Spheroid; Colorectal carcinoma},
language = {eng},
issn = {0753-3322},
journal = {BIOMEDICINE & PHARMACOTHERAPY},
title = {Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma},
url = {https://www.sciencedirect.com/science/article/pii/S075333222200854X?via%3Dihub},
volume = {153},
year = {2022}
}
TY - JOUR
ID - 2240067
AU - Hendrych, Michal - Říhová, Kamila - Adamová, Barbora - Hradil, Vojtěch - Stiborek, Marek - Vlček, Petr - Hermanová, Markéta - Vašíčková, Jana - Beneš, Petr - Šmarda, Jan - Kanický, Viktor - Preisler, Jan - Navrátilová, Jarmila
PY - 2022
TI - Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma
JF - BIOMEDICINE & PHARMACOTHERAPY
VL - 153
IS - September 2022
SP - 1-15
EP - 1-15
PB - ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
SN - 07533322
KW - Disulfiram/copper
KW - 5-fluorouracil
KW - Synergism
KW - Organotypic culture
KW - Spheroid
KW - Colorectal carcinoma
UR - https://www.sciencedirect.com/science/article/pii/S075333222200854X?via%3Dihub
N2 - Drug efficacy determined in preclinical research is difficult to transfer to clinical practice. This is mainly due to the use of oversimplified models omitting the effect of the tumor microenvironment and the presence of various cell types participating in the formation of tumors in vivo. In this study, we used robust three-dimensional models including spheroids grown from colon cancer cell lines and organotypic cultures prepared from the colorectal carcinoma tissue to test novel therapeutic strategies. We developed a multi-modal approach combining bright-field and fluorescence microscopy for evaluating drug effects on organotypic cultures. Combined treatment with 5-fluorouracil and disulfiram/copper efficiently eliminated cancer cells in these 3D models. Moreover, disulfiram/copper down-regulated the expression of markers associated with 5-fluorouracil resistance, such as thymidylate synthase and CD133/CD44. Thus, we propose combined therapy of 5-fluorouracil and disulfiram/copper for further testing as a treatment for colorectal carcinoma. In addition, we show that organotypic cultures are suitable models for anti-cancer drug testing.
ER -
HENDRYCH, Michal, Kamila ŘÍHOVÁ, Barbora ADAMOVÁ, Vojtěch HRADIL, Marek STIBOREK, Petr VLČEK, Markéta HERMANOVÁ, Jana VAŠÍČKOVÁ, Petr BENEŠ, Jan ŠMARDA, Viktor KANICKÝ, Jan PREISLER a Jarmila NAVRÁTILOVÁ. Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma. \textit{BIOMEDICINE \&{} PHARMACOTHERAPY}. Paris: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2022, roč.~153, September 2022, s.~1-15. ISSN~0753-3322. Dostupné z: https://dx.doi.org/10.1016/j.biopha.2022.113465.
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