Disulfiram increases the efficacy of 5-fluorouracil in
organotypic cultures of colorectal carcinoma

J 2022

Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma

HENDRYCH, Michal; Kamila ŘÍHOVÁ; Barbora ADAMOVÁ; Vojtěch HRADIL; Marek STIBOREK et al.

Základní údaje

Originální název

Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma

Autoři

Vydání

BIOMEDICINE & PHARMACOTHERAPY, Paris, ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2022, 0753-3322

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Francie

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 7.500

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/22:00127468

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Disulfiram/copper; 5-fluorouracil; Synergism; Organotypic culture; Spheroid; Colorectal carcinoma

Štítky

14110112, 14110120, 14313010, 14314010, podil, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 2. 2024 09:31, Mgr. Tereza Miškechová

Anotace

V originále

Drug efficacy determined in preclinical research is difficult to transfer to clinical practice. This is mainly due to the use of oversimplified models omitting the effect of the tumor microenvironment and the presence of various cell types participating in the formation of tumors in vivo. In this study, we used robust three-dimensional models including spheroids grown from colon cancer cell lines and organotypic cultures prepared from the colorectal carcinoma tissue to test novel therapeutic strategies. We developed a multi-modal approach combining bright-field and fluorescence microscopy for evaluating drug effects on organotypic cultures. Combined treatment with 5-fluorouracil and disulfiram/copper efficiently eliminated cancer cells in these 3D models. Moreover, disulfiram/copper down-regulated the expression of markers associated with 5-fluorouracil resistance, such as thymidylate synthase and CD133/CD44. Thus, we propose combined therapy of 5-fluorouracil and disulfiram/copper for further testing as a treatment for colorectal carcinoma. In addition, we show that organotypic cultures are suitable models for anti-cancer drug testing.

Návaznosti

GA18-16583S, projekt VaV

Název: Zobrazovací hmotnostní spektrometrie jednotlivých nanočástic

Investor: Grantová agentura ČR, Zobrazovací hmotnostní spektrometrie jednotlivých nanočástic

LX22NPO5102, projekt VaV

Název: Národní ústav pro výzkum rakoviny (Akronym: NÚVR)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní ústav pro výzkum rakoviny, 5.1 EXCELES

MUNI/A/1325/2021, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10 (Akronym: MBG10)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 10

MUNI/A/1408/2021, interní kód MU

Název: Organotypické kultury glioblastomu – personalizované testování protinádorové léčby

Investor: Masarykova univerzita, Organotypické kultury glioblastomu – personalizované testování protinádorové léčby

Přiložené soubory

Disulfiram_increases_the_efficacy_of_5-fluorouracil_in_organotypic_cultures_of_colorectal_carcinoma_ebirqswr.pdf

Citovat

HENDRYCH, Michal; Kamila ŘÍHOVÁ; Barbora ADAMOVÁ; Vojtěch HRADIL; Marek STIBOREK; Petr VLČEK; Markéta HERMANOVÁ; Jana VAŠÍČKOVÁ; Petr BENEŠ; Jan ŠMARDA; Viktor KANICKÝ; Jan PREISLER a Jarmila NAVRÁTILOVÁ. Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma. BIOMEDICINE & PHARMACOTHERAPY. Paris: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2022, roč. 153, September 2022, s. 1-15. ISSN 0753-3322. Dostupné z: https://doi.org/10.1016/j.biopha.2022.113465.

@article{2240067,
   author = {Hendrych, Michal and Říhová, Kamila and Adamová, Barbora and Hradil, Vojtěch and Stiborek, Marek and Vlček, Petr and Hermanová, Markéta and Vašíčková, Jana and Beneš, Petr and Šmarda, Jan and Kanický, Viktor and Preisler, Jan and Navrátilová, Jarmila},
   article_location = {Paris},
   article_number = {September 2022},
   doi = {https://doi.org/10.1016/j.biopha.2022.113465},
   keywords = {Disulfiram/copper; 5-fluorouracil; Synergism; Organotypic culture; Spheroid; Colorectal carcinoma},
   language = {eng},
   issn = {0753-3322},
   journal = {BIOMEDICINE & PHARMACOTHERAPY},
   title = {Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma},
   url = {https://www.sciencedirect.com/science/article/pii/S075333222200854X?via%3Dihub},
   volume = {153},
   year = {2022}
}

TY  - JOUR
ID  - 2240067
AU  - Hendrych, Michal - Říhová, Kamila - Adamová, Barbora - Hradil, Vojtěch - Stiborek, Marek - Vlček, Petr - Hermanová, Markéta - Vašíčková, Jana - Beneš, Petr - Šmarda, Jan - Kanický, Viktor - Preisler, Jan - Navrátilová, Jarmila
PY  - 2022
TI  - Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma
JF  - BIOMEDICINE & PHARMACOTHERAPY
VL  - 153
IS  - September 2022
SP  - 1-15
EP  - 1-15
PB  - ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
SN  - 07533322
KW  - Disulfiram/copper
KW  - 5-fluorouracil
KW  - Synergism
KW  - Organotypic culture
KW  - Spheroid
KW  - Colorectal carcinoma
UR  - https://www.sciencedirect.com/science/article/pii/S075333222200854X?via%3Dihub
N2  - Drug efficacy determined in preclinical research is difficult to transfer to clinical practice. This is mainly due to the use of oversimplified models omitting the effect of the tumor microenvironment and the presence of various cell types participating in the formation of tumors in vivo. In this study, we used robust three-dimensional models including spheroids grown from colon cancer cell lines and organotypic cultures prepared from the colorectal carcinoma tissue to test novel therapeutic strategies. We developed a multi-modal approach combining bright-field and fluorescence microscopy for evaluating drug effects on organotypic cultures. Combined treatment with 5-fluorouracil and disulfiram/copper efficiently eliminated cancer cells in these 3D models. Moreover, disulfiram/copper down-regulated the expression of markers associated with 5-fluorouracil resistance, such as thymidylate synthase and CD133/CD44. Thus, we propose combined therapy of 5-fluorouracil and disulfiram/copper for further testing as a treatment for colorectal carcinoma. In addition, we show that organotypic cultures are suitable models for anti-cancer drug testing.
ER  -

HENDRYCH, Michal; Kamila ŘÍHOVÁ; Barbora ADAMOVÁ; Vojtěch HRADIL; Marek STIBOREK; Petr VLČEK; Markéta HERMANOVÁ; Jana VAŠÍČKOVÁ; Petr BENEŠ; Jan ŠMARDA; Viktor KANICKÝ; Jan PREISLER a Jarmila NAVRÁTILOVÁ. Disulfiram increases the efficacy of 5-fluorouracil in organotypic cultures of colorectal carcinoma. \textit{BIOMEDICINE \&{} PHARMACOTHERAPY}. Paris: ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER, 2022, roč.~153, September 2022, s.~1-15. ISSN~0753-3322. Dostupné z: https://doi.org/10.1016/j.biopha.2022.113465.

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