Effects of gonadotropin-releasing hormone agonist administered
in microparticles on sperm quality and quantity, and plasma sex
steroid levels in northern pike

J 2022

Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike

KNOWLES, J.; S. BORYSHPOLETS; V. KHOLODNYY; D. RAHI; Jakub VYSLOUŽIL et al.

Základní údaje

Originální název

Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike

Autoři

KNOWLES, J.; S. BORYSHPOLETS; V. KHOLODNYY; D. RAHI; Jakub VYSLOUŽIL ; Jan MUSELÍK; V. STEJSKAL; J. KOURIL a P. PODHOREC

Vydání

ANIMAL, Amsterdam, Elsevier, 2022, 1751-7311

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.600

Kód RIV

RIV/00216224:14160/22:00129447

Organizační jednotka

Farmaceutická fakulta

DOI

https://doi.org/10.1016/j.animal.2021.100430

UT WoS

000826439700007

EID Scopus

2-s2.0-85121652691

Klíčová slova anglicky

Fish; Maturation; Sperm motility; Sperm production; Sustained drug release

Štítky

rivok, ÚFT

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 27. 2. 2023 13:23, JUDr. Sabina Gajdzioková

Anotace

V originále

Artificial reproduction of northern pike Esox lucius is impeded by the likelihood of obtaining only a small volume of sperm of inconsistent quality. A controlled-release hormone delivery system has the potential to enhance sperm production while avoiding multiple injections The objective of this study was to investigate the effects of mammalian gonadotropin-releasing hormone agonist (mGnRHa) incorporated into poly(lactic-co-glycolic acid) (PLGA) microparticles on milt production, spermatozoon characteristics, and secretion of 17β-estradiol (E2), 11-keto testosterone (11-KT), and testosterone in northern pike. Fish were divided into four groups and injected with 2 mg/kg BW carp pituitary extract (CPE), 20 µg/kg BW mGnRHa in PLGA microparticles, or 20 µg/kg BW mGnRHa plus 20 mg/kg BW metoclopramide (MET) in PLGA microparticles (PLGA + MET), along with a control group injected with 1 ml/kg 0.9% NaCl. At 48 h postinjection, the volume of milt produced was significantly greater in groups treated with CPE and PLGA + MET than in other groups. At 96 h postinjection, all hormone-treated groups exhibited significantly higher spermatozoon average velocity than recorded in the control group. Spermatozoon motility was significantly increased (P < 0.05) in the CPE and PLGA groups compared to baseline values. All treated groups showed significantly lower levels of 11-KT after the hormone injection compared to baseline values and to controls. Plasma testosterone levels increased in all hormone-treated groups. The use of PLGA microparticles, with or without metoclopramide, is suitable for use as a carrier of hormone treatments to regulate spermiation in mature northern pike.

Návaznosti

QK1810221, projekt VaV

Název: Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Investor: Ministerstvo zemědělství ČR, Využití mikročástic jako nosičů hormonálně aktivních látek v řízené reprodukci ryb.

Citovat

KNOWLES, J.; S. BORYSHPOLETS; V. KHOLODNYY; D. RAHI; Jakub VYSLOUŽIL; Jan MUSELÍK; V. STEJSKAL; J. KOURIL a P. PODHOREC. Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike. ANIMAL. Amsterdam: Elsevier, 2022, roč. 16, č. 1, s. 1-8. ISSN 1751-7311. Dostupné z: https://doi.org/10.1016/j.animal.2021.100430.

@article{2261737,
   author = {Knowles, J. and Boryshpolets, S. and Kholodnyy, V. and Rahi, D. and Vysloužil, Jakub and Muselík, Jan and Stejskal, V. and Kouril, J. and Podhorec, P.},
   article_location = {Amsterdam},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.animal.2021.100430},
   keywords = {Fish; Maturation; Sperm motility; Sperm production; Sustained drug release},
   language = {eng},
   issn = {1751-7311},
   journal = {ANIMAL},
   title = {Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike},
   url = {https://www.sciencedirect.com/science/article/pii/S1751731121002767?via%3Dihub},
   volume = {16},
   year = {2022}
}

TY  - JOUR
ID  - 2261737
AU  - Knowles, J. - Boryshpolets, S. - Kholodnyy, V. - Rahi, D. - Vysloužil, Jakub - Muselík, Jan - Stejskal, V. - Kouril, J. - Podhorec, P.
PY  - 2022
TI  - Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike
JF  - ANIMAL
VL  - 16
IS  - 1
SP  - 1-8
EP  - 1-8
PB  - Elsevier
SN  - 17517311
KW  - Fish
KW  - Maturation
KW  - Sperm motility
KW  - Sperm production
KW  - Sustained drug release
UR  - https://www.sciencedirect.com/science/article/pii/S1751731121002767?via%3Dihub
N2  - Artificial reproduction of northern pike Esox lucius is impeded by the likelihood of obtaining only a small volume of sperm of inconsistent quality. A controlled-release hormone delivery system has the potential to enhance sperm production while avoiding multiple injections The objective of this study was to investigate the effects of mammalian gonadotropin-releasing hormone agonist (mGnRHa) incorporated into poly(lactic-co-glycolic acid) (PLGA) microparticles on milt production, spermatozoon characteristics, and secretion of 17β-estradiol (E2), 11-keto testosterone (11-KT), and testosterone in northern pike. Fish were divided into four groups and injected with 2 mg/kg BW carp pituitary extract (CPE), 20 µg/kg BW mGnRHa in PLGA microparticles, or 20 µg/kg BW mGnRHa plus 20 mg/kg BW metoclopramide (MET) in PLGA microparticles (PLGA + MET), along with a control group injected with 1 ml/kg 0.9% NaCl. At 48 h postinjection, the volume of milt produced was significantly greater in groups treated with CPE and PLGA + MET than in other groups. At 96 h postinjection, all hormone-treated groups exhibited significantly higher spermatozoon average velocity than recorded in the control group. Spermatozoon motility was significantly increased (P < 0.05) in the CPE and PLGA groups compared to baseline values. All treated groups showed significantly lower levels of 11-KT after the hormone injection compared to baseline values and to controls. Plasma testosterone levels increased in all hormone-treated groups. The use of PLGA microparticles, with or without metoclopramide, is suitable for use as a carrier of hormone treatments to regulate spermiation in mature northern pike.
ER  -

KNOWLES, J.; S. BORYSHPOLETS; V. KHOLODNYY; D. RAHI; Jakub VYSLOUŽIL; Jan MUSELÍK; V. STEJSKAL; J. KOURIL a P. PODHOREC. Effects of gonadotropin-releasing hormone agonist administered in microparticles on sperm quality and quantity, and plasma sex steroid levels in northern pike. \textit{ANIMAL}. Amsterdam: Elsevier, 2022, roč.~16, č.~1, s.~1-8. ISSN~1751-7311. Dostupné z: https://doi.org/10.1016/j.animal.2021.100430.

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