Staphylococcus brunensis sp. nov. isolated from human clinical
specimens with a staphylococcal cassette chromosome-related
genomic island outside of the rlmH gene bearing the ccrDE
recombinase gene complex

J 2023

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

KOVAŘOVIC, Vojtěch, Adéla FINSTRLOVÁ, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC et. al.

Základní údaje

Originální název

Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex

Autoři

KOVAŘOVIC, Vojtěch (203 Česká republika, domácí), Adéla FINSTRLOVÁ (203 Česká republika, domácí), Ivo SEDLÁČEK (203 Česká republika, domácí), Petr PETRÁŠ (203 Česká republika), Pavel ŠVEC (203 Česká republika, domácí), Ivana MAŠLAŇOVÁ (203 Česká republika, domácí), Meina NEUMANN-SCHAAL (276 Německo), Ondrej ŠEDO (203 Česká republika, domácí), Tibor BOTKA (203 Česká republika, domácí), Eva STAŇKOVÁ (203 Česká republika, domácí), Jiří DOŠKAŘ (203 Česká republika, domácí) a Roman PANTŮČEK (203 Česká republika, garant, domácí)

Vydání

Microbiology Spectrum, Washington, DC, American Society for Microbiology, 2023, 2165-0497

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10606 Microbiology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.700 v roce 2022

Kód RIV

RIV/00216224:14310/23:00131714

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1128/spectrum.01342-23

UT WoS

001107303900086

Klíčová slova anglicky

coagulase-negative staphylococci; phylogenetic analysis; comparative genomics; mobile genetic elements; genomic islands; cassette chromosome recombinase; polyphasic taxonomy; gram-positive pathogens

Štítky

CF PROT, NIVB_PřF, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 11. 2024 21:19, Ing. Martina Blahová

Anotace

V originále

Novel species of coagulase-negative staphylococci, which could serve as reservoirs of virulence and antimicrobial resistance factors for opportunistic pathogens from the genus Staphylococcus, are recognized in human and animal specimens due to advances in diagnostic techniques. Here, we used whole-genome sequencing, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize five coagulase-negative strains from the Staphylococcus haemolyticus phylogenetic clade obtained from human ear swabs, wounds, and bile. Based on the results of polyphasic taxonomy, we propose the species Staphylococcus brunensis sp. nov. (type strain NRL/St 16/872T = CCM 9024T = LMG 31872T = DSM 111349T). The genomic analysis revealed numerous variable genomic elements, including staphylococcal cassette chromosome (SCC), prophages, plasmids, and a unique 18.8 kb-long genomic island SbCIccrDE integrated into the ribosomal protein L7 serine acetyltransferase gene rimL. SbCIccrDE has a cassette chromosome recombinase (ccr) gene complex with a typical structure found in SCCs. Based on nucleotide and amino acid identity to other known ccr genes and the distinct integration site that differs from the canonical methyltransferase gene rlmH exploited by SCCs, we classified the ccr genes as novel variants, ccrDE. The comparative genomic analysis of SbCIccrDE with related islands shows that they can accumulate virulence and antimicrobial resistance factors creating novel resistance elements, which reflects the evolution of SCC. The spread of these resistance islands into established pathogens such as Staphylococcus aureus would pose a great threat to the healthcare system.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2018140, projekt VaV

Název: e-Infrastruktura CZ (Akronym: e-INFRA CZ)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, e-Infrastruktura CZ

LX22NPO5103, projekt VaV

Název: Národní institut virologie a bakteriologie (Akronym: NIVB)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní institut virologie a bakteriologie, 5.1 EXCELES

MUNI/A/1287/2022, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 11

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 11

NU21J-05-00035, projekt VaV

Název: Synergie lytických bakteriofágů a antibiotik v léčbě povrchových infekcí způsobených Staphylococcus aureus (Akronym: PAS-based therapy of S. aureus infections)

Investor: Ministerstvo zdravotnictví ČR, Synergy of lytic bacteriophages and antibiotics in the therapy of topical infections of Staphylococcus aureus, Podprogram 2 - juniorský - výzkumníci do 35 let

90140, velká výzkumná infrastruktura

Název: e-INFRA CZ

90242, velká výzkumná infrastruktura

Název: CIISB III

Přiložené soubory

spectrum.01342-23.pdf

Vyhledat podobné dokumenty

2312638.pdf

Vyhledat podobné dokumenty

Citovat

KOVAŘOVIC, Vojtěch, Adéla FINSTRLOVÁ, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC, Ivana MAŠLAŇOVÁ, Meina NEUMANN-SCHAAL, Ondrej ŠEDO, Tibor BOTKA, Eva STAŇKOVÁ, Jiří DOŠKAŘ a Roman PANTŮČEK. Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex. Microbiology Spectrum. Washington, DC: American Society for Microbiology, 2023, roč. 11, č. 5, s. 1-22. ISSN 2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.01342-23.

@article{2312638,
   author = {Kovařovic, Vojtěch and Finstrlová, Adéla and Sedláček, Ivo and Petráš, Petr and Švec, Pavel and Mašlaňová, Ivana and NeumannandSchaal, Meina and Šedo, Ondrej and Botka, Tibor and Staňková, Eva and Doškař, Jiří and Pantůček, Roman},
   article_location = {Washington, DC},
   article_number = {5},
   doi = {http://dx.doi.org/10.1128/spectrum.01342-23},
   keywords = {coagulase-negative staphylococci; phylogenetic analysis; comparative genomics; mobile genetic elements; genomic islands; cassette chromosome recombinase; polyphasic taxonomy; gram-positive pathogens},
   language = {eng},
   issn = {2165-0497},
   journal = {Microbiology Spectrum},
   title = {Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex},
   url = {https://doi.org/10.1128/spectrum.01342-23},
   volume = {11},
   year = {2023}
}

TY  - JOUR
ID  - 2312638
AU  - Kovařovic, Vojtěch - Finstrlová, Adéla - Sedláček, Ivo - Petráš, Petr - Švec, Pavel - Mašlaňová, Ivana - Neumann-Schaal, Meina - Šedo, Ondrej - Botka, Tibor - Staňková, Eva - Doškař, Jiří - Pantůček, Roman
PY  - 2023
TI  - Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex
JF  - Microbiology Spectrum
VL  - 11
IS  - 5
SP  - 1-22
EP  - 1-22
PB  - American Society for Microbiology
SN  - 21650497
KW  - coagulase-negative staphylococci
KW  - phylogenetic analysis
KW  - comparative genomics
KW  - mobile genetic elements
KW  - genomic islands
KW  - cassette chromosome recombinase
KW  - polyphasic taxonomy
KW  - gram-positive pathogens
UR  - https://doi.org/10.1128/spectrum.01342-23
N2  - Novel species of coagulase-negative staphylococci, which could serve as reservoirs of virulence and antimicrobial resistance factors for opportunistic pathogens from the genus Staphylococcus, are recognized in human and animal specimens due to advances in diagnostic techniques. Here, we used whole-genome sequencing, extensive biotyping, MALDI-TOF mass spectrometry, and chemotaxonomy to characterize five coagulase-negative strains from the Staphylococcus haemolyticus phylogenetic clade obtained from human ear swabs, wounds, and bile. Based on the results of polyphasic taxonomy, we propose the species Staphylococcus brunensis sp. nov. (type strain NRL/St 16/872T = CCM 9024T = LMG 31872T = DSM 111349T). The genomic analysis revealed numerous variable genomic elements, including staphylococcal cassette chromosome (SCC), prophages, plasmids, and a unique 18.8 kb-long genomic island SbCIccrDE integrated into the ribosomal protein L7 serine acetyltransferase gene rimL. SbCIccrDE has a cassette chromosome recombinase (ccr) gene complex with a typical structure found in SCCs. Based on nucleotide and amino acid identity to other known ccr genes and the distinct integration site that differs from the canonical methyltransferase gene rlmH exploited by SCCs, we classified the ccr genes as novel variants, ccrDE. The comparative genomic analysis of SbCIccrDE with related islands shows that they can accumulate virulence and antimicrobial resistance factors creating novel resistance elements, which reflects the evolution of SCC. The spread of these resistance islands into established pathogens such as Staphylococcus aureus would pose a great threat to the healthcare system.
ER  -

KOVAŘOVIC, Vojtěch, Adéla FINSTRLOVÁ, Ivo SEDLÁČEK, Petr PETRÁŠ, Pavel ŠVEC, Ivana MAŠLAŇOVÁ, Meina NEUMANN-SCHAAL, Ondrej ŠEDO, Tibor BOTKA, Eva STAŇKOVÁ, Jiří DOŠKAŘ a Roman PANTŮČEK. Staphylococcus brunensis sp. nov. isolated from human clinical specimens with a staphylococcal cassette chromosome-related genomic island outside of the rlmH gene bearing the ccrDE recombinase gene complex. \textit{Microbiology Spectrum}. Washington, DC: American Society for Microbiology, 2023, roč.~11, č.~5, s.~1-22. ISSN~2165-0497. Dostupné z: https://dx.doi.org/10.1128/spectrum.01342-23.

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