Immunohistochemical localization of laminin-1 in the acellular
nerve grafts is associated with migrating Schwann cells which
display corresponding integrin receptors

J 1999

Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Ilona KLUSÁKOVÁ; Ladislav HOUŠŤAVA et al.

Základní údaje

Originální název

Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors

Název česky

Imunohistochemická analýza lamininu v acelulárním nervovém štěpu

Autoři

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Ilona KLUSÁKOVÁ; Ladislav HOUŠŤAVA a Pavel HANINEC

Vydání

General Physiology and Biophysics, Bratislava, Veda, 1999, 0231-5882

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30000 3. Medical and Health Sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.400

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/99:00001800

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration

Štítky

acellular nerve graft, Integrins, Laminin-1, Schwann cell migration

Změněno: 17. 6. 2009 12:15, prof. RNDr. Petr Dubový, CSc.

Anotace

ORIG CZ

V originále

The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.

Česky

Imunohistochemická detekce lamininu-1, collagen-IV, a6 a b1 řetězce integrinů byla studována v acelulárním segmentu nervu

Citovat

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Ilona KLUSÁKOVÁ; Ladislav HOUŠŤAVA a Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. General Physiology and Biophysics. Bratislava: Veda, 1999, roč. 18, č. 1, s. 63-65. ISSN 0231-5882.

@article{234931,
   author = {Dubový, Petr and Svíženská, Ivana and Jančálek, Radim and Klusáková, Ilona and Houšťava, Ladislav and Haninec, Pavel},
   article_location = {Bratislava},
   article_number = {1},
   keywords = {Acellular nerve graft; Integrins; Laminin-1; Schwann cell migration},
   language = {eng},
   issn = {0231-5882},
   journal = {General Physiology and Biophysics},
   title = {Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors},
   volume = {18},
   year = {1999}
}

TY  - JOUR
ID  - 234931
AU  - Dubový, Petr - Svíženská, Ivana - Jančálek, Radim - Klusáková, Ilona - Houšťava, Ladislav - Haninec, Pavel
PY  - 1999
TI  - Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors
JF  - General Physiology and Biophysics
VL  - 18
IS  - 1
SP  - 63
EP  - 63
PB  - Veda
SN  - 02315882
KW  - Acellular nerve graft
KW  - Integrins
KW  - Laminin-1
KW  - Schwann cell migration
N2  - The presence of laminin-1, collagen-IV, a6 and b1 integrin chains was detected by indirect immunohistochemistry using biotin/streptavidin/HRP or gold-conjugated secondary antibody at the light and electron microscope level, respectively. Cryo-treated segment of the peripheral stump without living Schwann cells (S-100-) did not display immunoreactivity for laminin-1 and integrin's chains, while the migrating Schwann cells in the marginal regions were immunostained for the antigens. Isolated acellular nerve segments protected from migration of Schwann cells (S-100-) exhibited laminin-l-, b1-, and a6- integrin chains immunoreactivities. Position of the basal lamina was verified by collagen-IV+ immunoreactivity. Results indicate that presence of the laminin in the peripheral nerve is related with living Schwann cells.
ER  -

DUBOVÝ, Petr; Ivana SVÍŽENSKÁ; Radim JANČÁLEK; Ilona KLUSÁKOVÁ; Ladislav HOUŠŤAVA a Pavel HANINEC. Immunohistochemical localization of laminin-1 in the acellular nerve grafts is associated with migrating Schwann cells which display corresponding integrin receptors. \textit{General Physiology and Biophysics}. Bratislava: Veda, 1999, roč.~18, č.~1, s.~63-65. ISSN~0231-5882.

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