Coculture model with CD40L, IL4 and IL21 for study chronic
lymphocytic leukemia proliferation.

a 2023

Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.

HOFERKOVÁ, Eva; Soňa KADÁKOVÁ; Václav ŠEDA; Jan VERNER; Miroslav BOUDNÝ et al.

Základní údaje

Originální název

Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.

Autoři

Vydání

CEITEC PhD Conference 2023, Brno, 2023

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

30204 Oncology

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/23:00132976

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Chronic lymphocytic leukemia; CD40L; IL4; IL21; inhibitors; proliferation

Štítky

rivok

Příznaky

Recenzováno

Změněno: 22. 12. 2025 11:26, Mgr. Petra Trembecká, Ph.D.

Anotace

V originále

Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.

Návaznosti

MUNI/A/1330/2021, interní kód MU

Název: Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX (Akronym: VýDiTeHeMa IX)

Investor: Masarykova univerzita, Nové přístupy ve výzkumu, diagnostice a terapii hematologických malignit IX

NU23-08-00448, projekt VaV

Název: VÝVOJ MODELŮ PRO STUDIUM MIKROPROSTŘEDÍ A TERAPEUTICKÉ REZISTENCE U B BUNĚČNÝCH MALIGNIT

Investor: Ministerstvo zdravotnictví ČR, Vývoj modelů pro studium mikroprostředí a terapeutické rezistence u B buněčných malignit, Podprogram 1 - standardní

802644, interní kód MU

Název: Signaling propensity in the microenvironment of B cell chronic lymphocytic leukemia (Akronym: LeukemiaEnviron)

Investor: Evropská unie, Signaling propensity in the microenvironment of B cell chronic lymphocytic leukemia, ERC (Excellent Science)

Citovat

HOFERKOVÁ, Eva; Soňa KADÁKOVÁ; Václav ŠEDA; Jan VERNER; Miroslav BOUDNÝ; Gabriela MLADONICKÁ PAVLASOVÁ; Tomáš LOJA; Daniel FILIP; Josef VEČEŘA; Květoslava MATULOVÁ; Hana SKUHROVÁ FRANCOVÁ; Sonali SHARMA; Laura ONDRIŠOVÁ; Veronika PALUŠOVÁ; Lucy VOJTOVÁ; Leoš KŘEN; Michael DOUBEK a Marek MRÁZ. Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation. In CEITEC PhD Conference 2023, Brno. 2023.

@proceedings{2360009,
   author = {Hoferková, Eva and Kadáková, Soňa and Šeda, Václav and Verner, Jan and Boudný, Miroslav and Mladonická Pavlasová, Gabriela and Loja, Tomáš and Filip, Daniel and Večeřa, Josef and Matulová, Květoslava and Skuhrová Francová, Hana and Sharma, Sonali and Ondrišová, Laura and Palušová, Veronika and Vojtová, Lucy and Křen, Leoš and Doubek, Michael and Mráz, Marek},
   booktitle = {CEITEC PhD Conference 2023, Brno},
   keywords = {Chronic lymphocytic leukemia; CD40L; IL4; IL21; inhibitors; proliferation},
   language = {eng},
   title = {Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.},
   url = {https://www.ceitec.eu/ceitec-phd-conference-2023/a4489},
   year = {2023}
}

TY  - CONF
ID  - 2360009
AU  - Hoferková, Eva - Kadáková, Soňa - Šeda, Václav - Verner, Jan - Boudný, Miroslav - Mladonická Pavlasová, Gabriela - Loja, Tomáš - Filip, Daniel - Večeřa, Josef - Matulová, Květoslava - Skuhrová Francová, Hana - Sharma, Sonali - Ondrišová, Laura - Palušová, Veronika - Vojtová, Lucy - Křen, Leoš - Doubek, Michael - Mráz, Marek
PY  - 2023
TI  - Coculture model with CD40L, IL4 and IL21 for study chronic lymphocytic leukemia proliferation.
KW  - Chronic lymphocytic leukemia
KW  - CD40L
KW  - IL4
KW  - IL21
KW  - inhibitors
KW  - proliferation
UR  - https://www.ceitec.eu/ceitec-phd-conference-2023/a4489
N2  - Chronic lymphocytic leukemia (CLL) cells exhibit dynamic trafficking between the quiescent and activated states in the lymphoid microenvironment, where they proliferate in response to signals from CD4+ T cells. These signals can be mimicked in vitro with artificial stimulation by CD40L, IL4, and IL21. In this study, we generated a panel of HS5-derived cell lines expressing T cell factors, which induce CLL proliferation and support CLL survival. 30 Using a coculture model, microenvironment-targeting drugs with potential effects on CLL cell proliferation were tested. Specifically, clinically used inhibitors including ibrutinib and idelalisib, and some of them were found to significantly inhibit CLL proliferation, with a reduction of up to 50% in the number of proliferative cells observed. The effect was not influenced by increased apoptosis.
ER  -

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