DNA Quadruplex Structure with a Unique Cation Dependency

J 2024

DNA Quadruplex Structure with a Unique Cation Dependency

GAJARSKÝ, Martin; Petr STADLBAUER; Jiri SPONER; Anne Pauline Marie CUCCHIARINI; Michaela DOBROVOLNA et. al.

Základní údaje

Originální název

DNA Quadruplex Structure with a Unique Cation Dependency

Autoři

GAJARSKÝ, Martin; Petr STADLBAUER; Jiri SPONER; Anne Pauline Marie CUCCHIARINI; Michaela DOBROVOLNA; Vaclav BRAZDA; Jean-Louis MERGNY; Lukáš TRANTÍREK a Martina LENARČIČ ŽIVKOVIC

Vydání

Angewandte Chemie International Edition, WEINHEIM (GERMANY), Verlag Chemie, 2024, 1433-7851

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10400 1.4 Chemical sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 16.900

Kód RIV

RIV/00216224:14740/24:00138656

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1002/anie.202313226

UT WoS

001142229900001

EID Scopus

2-s2.0-85182464964

Klíčová slova anglicky

DNA; quadruplex; unique cation dependency; NMR spectroscopy; telomere

Štítky

CF NMR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 13. 3. 2025 10:23, Mgr. Eva Dubská

Anotace

V originále

DNA quadruplex structures provide an additional layer of regulatory control in genome maintenance and gene expression and are widely used in nanotechnology. We report the discovery of an unprecedented tetrastranded structure formed from a native G-rich DNA sequence originating from the telomeric region of Caenorhabditis elegans. The structure is defined by multiple properties that distinguish it from all other known DNA quadruplexes. Most notably, the formation of a stable so-called KNa-quadruplex (KNaQ) requires concurrent coordination of K+ and Na+ ions at two distinct binding sites. This structure provides novel insight into G-rich DNA folding under ionic conditions relevant to eukaryotic cell physiology and the structural evolution of telomeric DNA. It highlights the differences between the structural organization of human and nematode telomeric DNA, which should be considered when using C. elegans as a model in telomere biology, particularly in drug screening applications. Additionally, the absence/presence of KNaQ motifs in the host/parasite introduces an intriguing possibility of exploiting the KNaQ fold as a plausible antiparasitic drug target. The structure's unique shape and ion dependency and the possibility of controlling its folding by using low-molecular-weight ligands can be used for the design or discovery of novel recognition DNA elements and sensors.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

EF18_070/0009846, projekt VaV

Název: MSCAfellow2@MUNI

GX19-25982X, projekt VaV

Název: Analýza replikace enterovirů s využitím elektronové mikroskopie

Investor: Grantová agentura ČR, Structural study of enterovirus replication in situ

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

GAJARSKÝ, Martin; Petr STADLBAUER; Jiri SPONER; Anne Pauline Marie CUCCHIARINI; Michaela DOBROVOLNA; Vaclav BRAZDA; Jean-Louis MERGNY; Lukáš TRANTÍREK a Martina LENARČIČ ŽIVKOVIC. DNA Quadruplex Structure with a Unique Cation Dependency. Angewandte Chemie International Edition. WEINHEIM (GERMANY): Verlag Chemie, 2024, roč. 63, č. 7, s. 1-10. ISSN 1433-7851. Dostupné z: https://doi.org/10.1002/anie.202313226.

@article{2471717,
   author = {Gajarský, Martin and Stadlbauer, Petr and Sponer, Jiri and Cucchiarini, Anne Pauline Marie and Dobrovolna, Michaela and Brazda, Vaclav and Mergny, JeanandLouis and Trantírek, Lukáš and Lenarčič Živkovic, Martina},
   article_location = {WEINHEIM (GERMANY)},
   article_number = {7},
   doi = {https://doi.org/10.1002/anie.202313226},
   keywords = {DNA; quadruplex; unique cation dependency; NMR spectroscopy; telomere},
   language = {eng},
   issn = {1433-7851},
   journal = {Angewandte Chemie International Edition},
   title = {DNA Quadruplex Structure with a Unique Cation Dependency},
   url = {https://onlinelibrary.wiley.com/doi/10.1002/anie.202313226},
   volume = {63},
   year = {2024}
}

TY  - JOUR
ID  - 2471717
AU  - Gajarský, Martin - Stadlbauer, Petr - Sponer, Jiri - Cucchiarini, Anne Pauline Marie - Dobrovolna, Michaela - Brazda, Vaclav - Mergny, Jean-Louis - Trantírek, Lukáš - Lenarčič Živkovic, Martina
PY  - 2024
TI  - DNA Quadruplex Structure with a Unique Cation Dependency
JF  - Angewandte Chemie International Edition
VL  - 63
IS  - 7
SP  - 1-10
EP  - 1-10
PB  - Verlag Chemie
SN  - 14337851
KW  - DNA
KW  - quadruplex
KW  - unique cation dependency
KW  - NMR spectroscopy
KW  - telomere
UR  - https://onlinelibrary.wiley.com/doi/10.1002/anie.202313226
N2  - DNA quadruplex structures provide an additional layer of regulatory control in genome maintenance and gene expression and are widely used in nanotechnology. We report the discovery of an unprecedented tetrastranded structure formed from a native G-rich DNA sequence originating from the telomeric region of Caenorhabditis elegans. The structure is defined by multiple properties that distinguish it from all other known DNA quadruplexes. Most notably, the formation of a stable so-called KNa-quadruplex (KNaQ) requires concurrent coordination of K+ and Na+ ions at two distinct binding sites. This structure provides novel insight into G-rich DNA folding under ionic conditions relevant to eukaryotic cell physiology and the structural evolution of telomeric DNA. It highlights the differences between the structural organization of human and nematode telomeric DNA, which should be considered when using C. elegans as a model in telomere biology, particularly in drug screening applications. Additionally, the absence/presence of KNaQ motifs in the host/parasite introduces an intriguing possibility of exploiting the KNaQ fold as a plausible antiparasitic drug target. The structure's unique shape and ion dependency and the possibility of controlling its folding by using low-molecular-weight ligands can be used for the design or discovery of novel recognition DNA elements and sensors.
ER  -

GAJARSKÝ, Martin; Petr STADLBAUER; Jiri SPONER; Anne Pauline Marie CUCCHIARINI; Michaela DOBROVOLNA; Vaclav BRAZDA; Jean-Louis MERGNY; Lukáš TRANTÍREK a Martina LENARČIČ ŽIVKOVIC. DNA Quadruplex Structure with a Unique Cation Dependency. \textit{Angewandte Chemie International Edition}. WEINHEIM (GERMANY): Verlag Chemie, 2024, roč.~63, č.~7, s.~1-10. ISSN~1433-7851. Dostupné z: https://doi.org/10.1002/anie.202313226.

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