RNA G-quadruplex formation in biologically important
transcribed regions: can two-tetrad intramolecular RNA
quadruplexes be formed?

J 2024

RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed?

BASU, Pritha; Iva KEJNOVSKA; Martin GAJARSKÝ; Denis ŠUBERT; Tereza MIKEŠOVÁ et. al.

Základní údaje

Originální název

RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed?

Autoři

BASU, Pritha; Iva KEJNOVSKA; Martin GAJARSKÝ; Denis ŠUBERT; Tereza MIKEŠOVÁ; Daniel RENCIUK; Lukáš TRANTÍREK; Jean-Louis MERGNY a Michaela VORLICKOVA

Vydání

Nucleic Acids Research, Oxford, Oxford University Press, 2024, 0305-1048

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 13.100

Kód RIV

RIV/00216224:14740/24:00138803

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1093/nar/gkae927

UT WoS

001347348900001

EID Scopus

2-s2.0-85211392790

Klíčová slova anglicky

CIRCULAR-DICHROISM; DNA; SEQUENCE; DIVERSITY; STACKING; 5-UTR

Štítky

14313050, 143160, CF CRYO, CF NMR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 30. 7. 2025 10:02, Mgr. Eva Dubská

Anotace

V originále

G-quadruplexes (G4s) formed within RNA are emerging as promising targets for therapeutic intervention in cancer, neurodegenerative disorders and infectious diseases. Sequences containing a succession of short GG blocks, or uneven G-tract lengths unable to form three-tetrad G4s (GG motifs), are overwhelmingly more frequent than canonical motifs involving multiple GGG blocks. We recently showed that DNA is not able to form stable two-tetrad intramolecular parallel G4s. Whether RNA GG motifs can form intramolecular G4s under physiological conditions and play regulatory roles remains a burning question. In this study, we performed a systematic analysis and experimental evaluation of a number of biologically important RNA regions involving RNA GG motifs. We show that most of these motifs do not form stable intramolecular G4s but need to dimerize to form stable G4 structures. The strong tendency of RNA GG motif G4s to associate may participate in RNA-based aggregation under conditions of cellular stress.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

GF22-04242L, projekt VaV

Název: Struktura, biologie a medicínský potenciál ultra-krátké mimobuněčné DNA v krevní plasmě

Investor: Grantová agentura ČR, Struktura, biologie a medicínský potenciál ultra-krátké mimobuněčné DNA v krevní plasmě, Partnerská agentura (Německo)

90242, velká výzkumná infrastruktura

Název: CIISB III

Citovat

BASU, Pritha; Iva KEJNOVSKA; Martin GAJARSKÝ; Denis ŠUBERT; Tereza MIKEŠOVÁ; Daniel RENCIUK; Lukáš TRANTÍREK; Jean-Louis MERGNY a Michaela VORLICKOVA. RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed? Nucleic Acids Research. Oxford: Oxford University Press, 2024, roč. 52, č. 21, s. 13224-13242. ISSN 0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkae927.

@article{2475082,
   author = {Basu, Pritha and Kejnovska, Iva and Gajarský, Martin and Šubert, Denis and Mikešová, Tereza and Renciuk, Daniel and Trantírek, Lukáš and Mergny, JeanandLouis and Vorlickova, Michaela},
   article_location = {Oxford},
   article_number = {21},
   doi = {https://doi.org/10.1093/nar/gkae927},
   keywords = {CIRCULAR-DICHROISM; DNA; SEQUENCE; DIVERSITY; STACKING; 5-UTR},
   language = {eng},
   issn = {0305-1048},
   journal = {Nucleic Acids Research},
   title = {RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed?},
   url = {https://academic.oup.com/nar/article/52/21/13224/7874850},
   volume = {52},
   year = {2024}
}

TY  - JOUR
ID  - 2475082
AU  - Basu, Pritha - Kejnovska, Iva - Gajarský, Martin - Šubert, Denis - Mikešová, Tereza - Renciuk, Daniel - Trantírek, Lukáš - Mergny, Jean-Louis - Vorlickova, Michaela
PY  - 2024
TI  - RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed?
JF  - Nucleic Acids Research
VL  - 52
IS  - 21
SP  - 13224-13242
EP  - 13224-13242
PB  - Oxford University Press
SN  - 03051048
KW  - CIRCULAR-DICHROISM
KW  - DNA
KW  - SEQUENCE
KW  - DIVERSITY
KW  - STACKING
KW  - 5-UTR
UR  - https://academic.oup.com/nar/article/52/21/13224/7874850
N2  - G-quadruplexes (G4s) formed within RNA are emerging as promising targets for therapeutic intervention in cancer, neurodegenerative disorders and infectious diseases. Sequences containing a succession of short GG blocks, or uneven G-tract lengths unable to form three-tetrad G4s (GG motifs), are overwhelmingly more frequent than canonical motifs involving multiple GGG blocks. We recently showed that DNA is not able to form stable two-tetrad intramolecular parallel G4s. Whether RNA GG motifs can form intramolecular G4s under physiological conditions and play regulatory roles remains a burning question. In this study, we performed a systematic analysis and experimental evaluation of a number of biologically important RNA regions involving RNA GG motifs. We show that most of these motifs do not form stable intramolecular G4s but need to dimerize to form stable G4 structures. The strong tendency of RNA GG motif G4s to associate may participate in RNA-based aggregation under conditions of cellular stress.
ER  -

BASU, Pritha; Iva KEJNOVSKA; Martin GAJARSKÝ; Denis ŠUBERT; Tereza MIKEŠOVÁ; Daniel RENCIUK; Lukáš TRANTÍREK; Jean-Louis MERGNY a Michaela VORLICKOVA. RNA G-quadruplex formation in biologically important transcribed regions: can two-tetrad intramolecular RNA quadruplexes be formed? \textit{Nucleic Acids Research}. Oxford: Oxford University Press, 2024, roč.~52, č.~21, s.~13224-13242. ISSN~0305-1048. Dostupné z: https://doi.org/10.1093/nar/gkae927.

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