Ultrastructure of macromolecular assemblies contributing to
bacterial spore resistance revealed by in situ cryo-electron
tomography

J 2024

Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

BAUDA, Elda; Benoit GALLET; Jana MORAVCOVÁ; Gregory EFFANTIN; Helena CHAN et. al.

Základní údaje

Originální název

Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography

Autoři

BAUDA, Elda; Benoit GALLET; Jana MORAVCOVÁ; Gregory EFFANTIN; Helena CHAN; Jiří NOVÁČEK; Pierre-Henri JOUNEAU; Christopher D A RODRIGUES; Guy SCHOEHN; Christine MORISCOT a Cecile MORLOT

Vydání

Nature Communications, Berlin, Nature, 2024, 2041-1723

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30300 3.3 Health sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 15.700

Kód RIV

RIV/00216224:14740/24:00138862

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1038/s41467-024-45770-6

UT WoS

001162717700027

EID Scopus

2-s2.0-85185344033

Klíčová slova anglicky

BACILLUS-SUBTILIS; COAT; DNA; BEAM; TRANSFORMATIONS; VISUALIZATION; MORPHOGENESIS; PROTECTION; SAFA

Štítky

CF CRYO, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 3. 6. 2025 12:29, Mgr. Eva Dubská

Anotace

V originále

Bacterial spores owe their incredible resistance capacities to molecular structures that protect the cell content from external aggressions. Among the determinants of resistance are the quaternary structure of the chromosome and an extracellular shell made of proteinaceous layers (the coat), the assembly of which remains poorly understood. Here, in situ cryo-electron tomography on lamellae generated by cryo-focused ion beam micromachining provides insights into the ultrastructural organization of Bacillus subtilis sporangia. The reconstructed tomograms reveal that early during sporulation, the chromosome in the forespore adopts a toroidal structure harboring 5.5-nm thick fibers. At the same stage, coat proteins at the surface of the forespore form a stack of amorphous or structured layers with distinct electron density, dimensions and organization. By analyzing mutant strains using cryo-electron tomography and transmission electron microscopy on resin sections, we distinguish seven nascent coat regions with different molecular properties, and propose a model for the contribution of coat morphogenetic proteins.

Návaznosti

EF18_046/0015974, projekt VaV

Název: Modernizace České infrastruktury pro integrativní strukturní biologii

LM2023042, projekt VaV

Název: Česká infrastruktura pro integrativní strukturní biologii

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CIISB - Česká infrastruktura pro integrativní strukturní biologii

871037, interní kód MU

Název: iNEXT-Discovery: Infrastructure for transnational access and discovery in integrated structural biology (Akronym: iNEXT- Discovery)

Investor: Evropská unie, iNEXT-Discovery: Infrastructure for transnational access and discovery in integrated structural biology, RI Research Infrastructures (Excellent Science)

Citovat

BAUDA, Elda; Benoit GALLET; Jana MORAVCOVÁ; Gregory EFFANTIN; Helena CHAN; Jiří NOVÁČEK; Pierre-Henri JOUNEAU; Christopher D A RODRIGUES; Guy SCHOEHN; Christine MORISCOT a Cecile MORLOT. Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography. Nature Communications. Berlin: Nature, 2024, roč. 15, č. 1, s. 1-12. ISSN 2041-1723. Dostupné z: https://doi.org/10.1038/s41467-024-45770-6.

@article{2477158,
   author = {Bauda, Elda and Gallet, Benoit and Moravcová, Jana and Effantin, Gregory and Chan, Helena and Nováček, Jiří and Jouneau, PierreandHenri and Rodrigues, Christopher D A and Schoehn, Guy and Moriscot, Christine and Morlot, Cecile},
   article_location = {Berlin},
   article_number = {1},
   doi = {https://doi.org/10.1038/s41467-024-45770-6},
   keywords = {BACILLUS-SUBTILIS; COAT; DNA; BEAM; TRANSFORMATIONS; VISUALIZATION; MORPHOGENESIS; PROTECTION; SAFA},
   language = {eng},
   issn = {2041-1723},
   journal = {Nature Communications},
   title = {Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography},
   url = {https://www.nature.com/articles/s41467-024-45770-6},
   volume = {15},
   year = {2024}
}

TY  - JOUR
ID  - 2477158
AU  - Bauda, Elda - Gallet, Benoit - Moravcová, Jana - Effantin, Gregory - Chan, Helena - Nováček, Jiří - Jouneau, Pierre-Henri - Rodrigues, Christopher D A - Schoehn, Guy - Moriscot, Christine - Morlot, Cecile
PY  - 2024
TI  - Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography
JF  - Nature Communications
VL  - 15
IS  - 1
SP  - 1-12
EP  - 1-12
PB  - Nature
SN  - 20411723
KW  - BACILLUS-SUBTILIS
KW  - COAT
KW  - DNA
KW  - BEAM
KW  - TRANSFORMATIONS
KW  - VISUALIZATION
KW  - MORPHOGENESIS
KW  - PROTECTION
KW  - SAFA
UR  - https://www.nature.com/articles/s41467-024-45770-6
N2  - Bacterial spores owe their incredible resistance capacities to molecular structures that protect the cell content from external aggressions. Among the determinants of resistance are the quaternary structure of the chromosome and an extracellular shell made of proteinaceous layers (the coat), the assembly of which remains poorly understood. Here, in situ cryo-electron tomography on lamellae generated by cryo-focused ion beam micromachining provides insights into the ultrastructural organization of Bacillus subtilis sporangia. The reconstructed tomograms reveal that early during sporulation, the chromosome in the forespore adopts a toroidal structure harboring 5.5-nm thick fibers. At the same stage, coat proteins at the surface of the forespore form a stack of amorphous or structured layers with distinct electron density, dimensions and organization. By analyzing mutant strains using cryo-electron tomography and transmission electron microscopy on resin sections, we distinguish seven nascent coat regions with different molecular properties, and propose a model for the contribution of coat morphogenetic proteins.
ER  -

BAUDA, Elda; Benoit GALLET; Jana MORAVCOVÁ; Gregory EFFANTIN; Helena CHAN; Jiří NOVÁČEK; Pierre-Henri JOUNEAU; Christopher D A RODRIGUES; Guy SCHOEHN; Christine MORISCOT a Cecile MORLOT. Ultrastructure of macromolecular assemblies contributing to bacterial spore resistance revealed by in situ cryo-electron tomography. \textit{Nature Communications}. Berlin: Nature, 2024, roč.~15, č.~1, s.~1-12. ISSN~2041-1723. Dostupné z: https://doi.org/10.1038/s41467-024-45770-6.

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