Testing Sonication and Different Transport Media to Enhance
Microbial Detection in Hard-to-Heal Venous Leg Ulcers

J 2025

Testing Sonication and Different Transport Media to Enhance Microbial Detection in Hard-to-Heal Venous Leg Ulcers

HOLUBOVA, Adela; Niels A J CREMERS; Martin KRAUSE; Lada CETLOVA; Jana MATEJKOVA et al.

Základní údaje

Originální název

Testing Sonication and Different Transport Media to Enhance Microbial Detection in Hard-to-Heal Venous Leg Ulcers

Autoři

HOLUBOVA, Adela; Niels A J CREMERS; Martin KRAUSE; Lada CETLOVA; Jana MATEJKOVA a Andrea POKORNÁ

Vydání

International Wound Journal, Hoboken, Wiley-Blackwell, 2025, 1742-4801

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30216 Dermatology and venereal diseases

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.500 v roce 2024

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/25:00142691

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

ceramic wound dressing; hard-to-heal wound; hydroactive dressing; medical grade honey; quality of live; sonicate; swab; wounds

Štítky

14110611, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 25. 11. 2025 13:34, Mgr. Tereza Miškechová

Anotace

V originále

Hard-to-heal wounds are frequently associated with underlying conditions such as diabetes, vascular disease, and biofilm-related infections. Accurate identification of microbial origin is essential, but is often hindered by biofilms. This study evaluated whether sonication of wound dressings, combined with different sample transport methods, improves bacterial detection in venous leg ulcers. In a prospective observational case-control study, six patients with hard-to-heal venous leg ulcers received treatments with medical-grade honey (MGH) (n = 1), ceramic dressings - "Cerdak" (n = 2), or hydroactive dressings (n = 3). Three microbiological samples were collected per patient: (1) conventional wound swab (Levin's technique) in Amies medium; (2) sonicate fluid from used dressings transported in sterile tubes without medium; and (3) sonicate fluid in haemoculture tubes (BACT/ALERT). Total pathogen count and diversity were compared across sampling methods. Patient quality of life (QoL) was assessed using the Wound-QoL instrument. Sonication revealed additional pathogens not detected by conventional swabs. The highest number of pathogens was found in sonicate fluid transported in haemoculture tubes (n = 43), followed by swabs (n = 39) and sterile tube transport (n = 30). Adequate treatment significantly improved patients' QoL during the healing process. Dressing sonication, especially with haemoculture tube transport, enhances microbial identification and may improve diagnosis and management of hard-to-heal wounds.

Citovat

HOLUBOVA, Adela; Niels A J CREMERS; Martin KRAUSE; Lada CETLOVA; Jana MATEJKOVA a Andrea POKORNÁ. Testing Sonication and Different Transport Media to Enhance Microbial Detection in Hard-to-Heal Venous Leg Ulcers. International Wound Journal. Hoboken: Wiley-Blackwell, 2025, roč. 22, č. 11, s. 1-19. ISSN 1742-4801. Dostupné z: https://doi.org/10.1111/iwj.70778.

@article{2535506,
   author = {Holubova, Adela and Cremers, Niels A J and Krause, Martin and Cetlova, Lada and Matejkova, Jana and POKORNÁ, Andrea},
   article_location = {Hoboken},
   article_number = {11},
   doi = {https://doi.org/10.1111/iwj.70778},
   keywords = {ceramic wound dressing; hard-to-heal wound; hydroactive dressing; medical grade honey; quality of live; sonicate; swab; wounds},
   language = {eng},
   issn = {1742-4801},
   journal = {International Wound Journal},
   title = {Testing Sonication and Different Transport Media to Enhance Microbial Detection in Hard-to-Heal Venous Leg Ulcers},
   url = {https://onlinelibrary.wiley.com/doi/10.1111/iwj.70778},
   volume = {22},
   year = {2025}
}

TY  - JOUR
ID  - 2535506
AU  - Holubova, Adela - Cremers, Niels A J - Krause, Martin - Cetlova, Lada - Matejkova, Jana - POKORNÁ, Andrea
PY  - 2025
TI  - Testing Sonication and Different Transport Media to Enhance Microbial Detection in Hard-to-Heal Venous Leg Ulcers
JF  - International Wound Journal
VL  - 22
IS  - 11
SP  - 1-19
EP  - 1-19
PB  - Wiley-Blackwell
SN  - 17424801
KW  - ceramic wound dressing
KW  - hard-to-heal wound
KW  - hydroactive dressing
KW  - medical grade honey
KW  - quality of live
KW  - sonicate
KW  - swab
KW  - wounds
UR  - https://onlinelibrary.wiley.com/doi/10.1111/iwj.70778
N2  - Hard-to-heal wounds are frequently associated with underlying conditions such as diabetes, vascular disease, and biofilm-related infections. Accurate identification of microbial origin is essential, but is often hindered by biofilms. This study evaluated whether sonication of wound dressings, combined with different sample transport methods, improves bacterial detection in venous leg ulcers. In a prospective observational case-control study, six patients with hard-to-heal venous leg ulcers received treatments with medical-grade honey (MGH) (n = 1), ceramic dressings - "Cerdak" (n = 2), or hydroactive dressings (n = 3). Three microbiological samples were collected per patient: (1) conventional wound swab (Levin's technique) in Amies medium; (2) sonicate fluid from used dressings transported in sterile tubes without medium; and (3) sonicate fluid in haemoculture tubes (BACT/ALERT). Total pathogen count and diversity were compared across sampling methods. Patient quality of life (QoL) was assessed using the Wound-QoL instrument. Sonication revealed additional pathogens not detected by conventional swabs. The highest number of pathogens was found in sonicate fluid transported in haemoculture tubes (n = 43), followed by swabs (n = 39) and sterile tube transport (n = 30). Adequate treatment significantly improved patients' QoL during the healing process. Dressing sonication, especially with haemoculture tube transport, enhances microbial identification and may improve diagnosis and management of hard-to-heal wounds.
ER  -

HOLUBOVA, Adela; Niels A J CREMERS; Martin KRAUSE; Lada CETLOVA; Jana MATEJKOVA a Andrea POKORNÁ. Testing Sonication and Different Transport Media to Enhance Microbial Detection in Hard-to-Heal Venous Leg Ulcers. \textit{International Wound Journal}. Hoboken: Wiley-Blackwell, 2025, roč.~22, č.~11, s.~1-19. ISSN~1742-4801. Dostupné z: https://doi.org/10.1111/iwj.70778.

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