Development and Validation of a Rapid and Simple UHPLC-MS/MS
Method for the Determination of Colchicine in Human Plasma

J 2025

Development and Validation of a Rapid and Simple UHPLC-MS/MS Method for the Determination of Colchicine in Human Plasma

ZIDEKOVA, Nela; Kristian PRSO; Marek PRSO; Milos JESENAK; Oldřich FARSA et al.

Základní údaje

Originální název

Development and Validation of a Rapid and Simple UHPLC-MS/MS Method for the Determination of Colchicine in Human Plasma

Autoři

ZIDEKOVA, Nela; Kristian PRSO; Marek PRSO; Milos JESENAK; Oldřich FARSA a Martin KERTYS

Vydání

BIOMEDICAL CHROMATOGRAPHY, HOBOKEN, WILEY, 2025, 0269-3879

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30104 Pharmacology and pharmacy

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

Wiley

Impakt faktor

Impact factor: 1.700 v roce 2024

Označené pro přenos do RIV

Ano

Organizační jednotka

Farmaceutická fakulta

Klíčová slova anglicky

colchicine; familial Mediterranean fever; liquid chromatography-tandem mass spectrometry; phospholipid removal; therapeutic drug monitoring

Štítky

rivok, ÚChL

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 11. 3. 2026 14:57, Mgr. Irena Doubková

Anotace

V originále

Colchicine is a naturally occurring alkaloid primarily derived from plants of the Colchicum genus, which is used to treat gout and serve as a frontline therapy for various inflammatory conditions, including familial Mediterranean fever. Although it is not recommended for routine therapeutic drug monitoring, there are situations where it may be beneficial, such as in dose adjustments. The present study introduces an LC-MS/MS method for quantifying colchicine in human plasma. A one-step extraction procedure employing an Ostro plate was applied, and the extracts were analyzed using gradient elution followed by detection on a mass spectrometer in multiple reaction monitoring mode. Our method offers several advantages, including a low sample volume and a run time of only 3 min. It demonstrates sufficient linearity to quantify low and high concentrations of colchicine in human plasma samples. The method was successfully validated in accordance with the ICH guideline M10 on bioanalytical method validation, covering selectivity, linearity, limit of quantification, accuracy, precision, dilution integrity, carry-over effect, matrix effects, extraction recovery, and stability over a concentration range of 0.05-100 ng/mL. The fully developed and validated method was applied to determine colchicine in plasma samples from patients diagnosed with familial Mediterranean fever.

Citovat

ZIDEKOVA, Nela; Kristian PRSO; Marek PRSO; Milos JESENAK; Oldřich FARSA a Martin KERTYS. Development and Validation of a Rapid and Simple UHPLC-MS/MS Method for the Determination of Colchicine in Human Plasma. BIOMEDICAL CHROMATOGRAPHY. HOBOKEN: WILEY, 2025, roč. 39, č. 11, s. 1-9. ISSN 0269-3879. Dostupné z: https://doi.org/10.1002/bmc.70222.

@article{2554530,
   author = {Zidekova, Nela and Prso, Kristian and Prso, Marek and Jesenak, Milos and Farsa, Oldřich and Kertys, Martin},
   article_location = {HOBOKEN},
   article_number = {11},
   doi = {https://doi.org/10.1002/bmc.70222},
   keywords = {colchicine; familial Mediterranean fever; liquid chromatography-tandem mass spectrometry; phospholipid removal; therapeutic drug monitoring},
   language = {eng},
   issn = {0269-3879},
   journal = {BIOMEDICAL CHROMATOGRAPHY},
   title = {Development and Validation of a Rapid and Simple UHPLC-MS/MS Method for the Determination of Colchicine in Human Plasma},
   url = {https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70222?getft_integrator=clarivate&src=getftr&utm_source=clarivate},
   volume = {39},
   year = {2025}
}

TY  - JOUR
ID  - 2554530
AU  - Zidekova, Nela - Prso, Kristian - Prso, Marek - Jesenak, Milos - Farsa, Oldřich - Kertys, Martin
PY  - 2025
TI  - Development and Validation of a Rapid and Simple UHPLC-MS/MS Method for the Determination of Colchicine in Human Plasma
JF  - BIOMEDICAL CHROMATOGRAPHY
VL  - 39
IS  - 11
SP  - 1-9
EP  - 1-9
PB  - WILEY
SN  - 02693879
KW  - colchicine
KW  - familial Mediterranean fever
KW  - liquid chromatography-tandem mass spectrometry
KW  - phospholipid removal
KW  - therapeutic drug monitoring
UR  - https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/bmc.70222?getft_integrator=clarivate&src=getftr&utm_source=clarivate
N2  - Colchicine is a naturally occurring alkaloid primarily derived from plants of the Colchicum genus, which is used to treat gout and serve as a frontline therapy for various inflammatory conditions, including familial Mediterranean fever. Although it is not recommended for routine therapeutic drug monitoring, there are situations where it may be beneficial, such as in dose adjustments. The present study introduces an LC-MS/MS method for quantifying colchicine in human plasma. A one-step extraction procedure employing an Ostro plate was applied, and the extracts were analyzed using gradient elution followed by detection on a mass spectrometer in multiple reaction monitoring mode. Our method offers several advantages, including a low sample volume and a run time of only 3 min. It demonstrates sufficient linearity to quantify low and high concentrations of colchicine in human plasma samples. The method was successfully validated in accordance with the ICH guideline M10 on bioanalytical method validation, covering selectivity, linearity, limit of quantification, accuracy, precision, dilution integrity, carry-over effect, matrix effects, extraction recovery, and stability over a concentration range of 0.05-100 ng/mL. The fully developed and validated method was applied to determine colchicine in plasma samples from patients diagnosed with familial Mediterranean fever.
ER  -

ZIDEKOVA, Nela; Kristian PRSO; Marek PRSO; Milos JESENAK; Oldřich FARSA a Martin KERTYS. Development and Validation of a Rapid and Simple UHPLC-MS/MS Method for the Determination of Colchicine in Human Plasma. \textit{BIOMEDICAL CHROMATOGRAPHY}. HOBOKEN: WILEY, 2025, roč.~39, č.~11, s.~1-9. ISSN~0269-3879. Dostupné z: https://doi.org/10.1002/bmc.70222.

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